ID   PTASE_MBVLF             Reviewed;         657 AA.
AC   Q9YPD6;
DT   30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=Putative serine protease;
DE            EC=3.4.21.-;
GN   ORFNames=ORF2;
OS   Mushroom bacilliform virus (isolate Australia/AUS LF-1) (MBV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Sobelivirales; Barnaviridae; Barnavirus.
OX   NCBI_TaxID=650482;
OH   NCBI_TaxID=5341; Agaricus bisporus (White button mushroom).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=8030251; DOI=10.1006/viro.1994.1412;
RA   Revill P.A., Davidson A.D., Wright P.J.;
RT   "The nucleotide sequence and genome organization of mushroom bacilliform
RT   virus: a single-stranded RNA virus of Agaricus bisporus (Lange) Imbach.";
RL   Virology 202:904-911(1994).
CC   -!- FUNCTION: Putative serine protease. {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Host membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the peptidase S39B family. {ECO:0000305}.
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DR   EMBL; U07551; AAA53089.1; -; Genomic_RNA.
DR   RefSeq; NP_042509.1; NC_001633.1.
DR   SMR; Q9YPD6; -.
DR   GeneID; 1497108; -.
DR   KEGG; vg:1497108; -.
DR   Proteomes; UP000006824; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   GO; GO:0016032; P:viral process; IEA:InterPro.
DR   Gene3D; 2.40.10.10; -; 2.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR000382; Peptidase_S39B_luteovirus.
DR   Pfam; PF02122; Peptidase_S39; 1.
DR   SUPFAM; SSF50494; SSF50494; 1.
DR   PROSITE; PS51868; PEPTIDASE_S39; 1.
PE   3: Inferred from homology;
KW   Host membrane; Hydrolase; Membrane; Protease; Reference proteome;
KW   Serine protease; Transmembrane; Transmembrane helix.
FT   CHAIN           1..657
FT                   /note="Putative serine protease"
FT                   /id="PRO_0000402445"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        46..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        109..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          239..434
FT                   /note="Peptidase S39"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01216"
FT   REGION          513..605
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        546..560
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        566..584
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        585..599
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        284
FT                   /note="For protease activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01216"
FT   ACT_SITE        318
FT                   /note="For protease activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01216"
FT   ACT_SITE        386
FT                   /note="For protease activity"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01216"
SQ   SEQUENCE   657 AA;  73415 MW;  A55C331F24675888 CRC64;
     MSKYLATSVR LCLMVCIVGW LLMPSYKELD GWCSSLSSLE RDKSNWLLTG LSTWFCIVPS
     GTDQSSLVSY FSPLEKLSKF VQDLDLDFVK LWWLETITLI NTLNTTEKLL SGVTFSVVLW
     YPRILVTVLM LVWKLWFPVR FLVVASSLLC LRILVWPFEV IADVILETCA WFTRKYHKLM
     DVIEDLMMIP QRVMEWCSGN TAKMVVPTVA SCVSESIESK LDRILMALGR KGTVLEAAQP
     GSDFVECEQW PNGLVAIRRH DGRIVGMGFL VVLNGKWRLV TAAHVARECK RGIMLSAGID
     SKTVTFQDLD VVLQTQVDAC IMNVPAGTAA SLGVRKVVIN RTPSESKVVR TYGYNSGKFC
     MSEGLVGTTS ANMGFRHGCS TLRGWSGTPI YRDNKVVGIH SRCNGIYENF GLSLDLLVGR
     LESEETDRYA RTMEEFNTED RPVTPPMEFS WEFEEKFERV RSTRKSFARI ESEVATFTAT
     KLSGFDWTDD APMDFDELPV FESTMVSVFQ ERPLGGLPIS NGNKAEEKKI TSEALEPSKS
     STPEAAKHTR RRRRNKKKSK NSETGHGPEE QSQQQSRPSS PIPDDSAPVS SPPVSPPSTG
     SVPKSWTQAY TQKLVLLLGS MDGQSKEKVD LAILEAKSFA SALFPPSKPK SSEESEK