PTC1_CAEEL
ID PTC1_CAEEL Reviewed; 1408 AA.
AC Q09614;
DT 24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 02-SEP-2008, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Protein patched homolog 1;
GN Name=ptc-1; ORFNames=ZK675.1;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=10921907;
RA Kuwabara P.E., Lee M.-H., Schedl T., Jefferis G.S.X.E.;
RT "A C. elegans patched gene, ptc-1, functions in germ-line cytokinesis.";
RL Genes Dev. 14:1933-1944(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-599; ASN-1026 AND ASN-1036, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=Bristol N2;
RX PubMed=17761667; DOI=10.1074/mcp.m600392-mcp200;
RA Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T.,
RA Taoka M., Takahashi N., Isobe T.;
RT "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis
RT elegans and suggests an atypical translocation mechanism for integral
RT membrane proteins.";
RL Mol. Cell. Proteomics 6:2100-2109(2007).
CC -!- FUNCTION: Required but not essential for cytokinesis of mitotically
CC proliferating germ cells. {ECO:0000269|PubMed:10921907}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:10921907}; Multi-
CC pass membrane protein {ECO:0000269|PubMed:10921907}. Note=Highly
CC concentrated in a punctate pattern at the apices and internal membranes
CC adjacent to the rachis. Expression is more intense in the distal
CC proliferative region and in oocytes of the gonad arm.
CC -!- TISSUE SPECIFICITY: Germ line and its progenitors.
CC {ECO:0000269|PubMed:10921907}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC {ECO:0000269|PubMed:10921907}.
CC -!- MISCELLANEOUS: Membrane proteins Patch and Smoothened form a receptor
CC complex that binds Hedgehog morphogens. Despite the lack of Hh and Smo
CC homologs, ptc-1 is still found to be functional. Absence of ptc-1 leads
CC to the formation of multinucleate germ cells and sterility.
CC {ECO:0000269|PubMed:10921907}.
CC -!- SIMILARITY: Belongs to the patched family. {ECO:0000305}.
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DR EMBL; Z46812; CAA86843.2; -; Genomic_DNA.
DR PIR; T27969; T27969.
DR RefSeq; NP_495662.2; NM_063261.4.
DR AlphaFoldDB; Q09614; -.
DR SMR; Q09614; -.
DR BioGRID; 39607; 1.
DR STRING; 6239.ZK675.1.1; -.
DR iPTMnet; Q09614; -.
DR EPD; Q09614; -.
DR PaxDb; Q09614; -.
DR PeptideAtlas; Q09614; -.
DR EnsemblMetazoa; ZK675.1a.1; ZK675.1a.1; WBGene00004208.
DR GeneID; 174274; -.
DR KEGG; cel:CELE_ZK675.1; -.
DR UCSC; ZK675.1.1; c. elegans.
DR CTD; 174274; -.
DR WormBase; ZK675.1a; CE42497; WBGene00004208; ptc-1.
DR eggNOG; KOG1935; Eukaryota.
DR GeneTree; ENSGT00940000167988; -.
DR HOGENOM; CLU_002506_1_0_1; -.
DR InParanoid; Q09614; -.
DR OMA; YGWHEYE; -.
DR OrthoDB; 1190129at2759; -.
DR PhylomeDB; Q09614; -.
DR PRO; PR:Q09614; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00004208; Expressed in adult organism and 4 other tissues.
DR ExpressionAtlas; Q09614; baseline and differential.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:WormBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0097108; F:hedgehog family protein binding; IBA:GO_Central.
DR GO; GO:0008158; F:hedgehog receptor activity; IBA:GO_Central.
DR GO; GO:0005119; F:smoothened binding; IBA:GO_Central.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0018996; P:molting cycle, collagen and cuticulin-based cuticle; IMP:WormBase.
DR GO; GO:0007224; P:smoothened signaling pathway; IBA:GO_Central.
DR InterPro; IPR003392; Ptc/Disp.
DR InterPro; IPR000731; SSD.
DR Pfam; PF02460; Patched; 1.
DR PROSITE; PS50156; SSD; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Glycoprotein; Membrane; Mitosis;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..1408
FT /note="Protein patched homolog 1"
FT /id="PRO_0000205969"
FT TOPO_DOM 1..136
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 158..649
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 650..670
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 671..686
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 687..707
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 708..709
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 710..730
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 731..765
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 766..786
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 787..795
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 796..816
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 817..901
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 902..922
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 923..1175
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1176..1196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1197..1217
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1218..1238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1239..1259
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1260..1276
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1277..1297
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1298..1305
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1306..1326
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1327..1408
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT DOMAIN 654..816
FT /note="SSD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00199"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 455..479
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1342..1408
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1342..1362
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1381..1401
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 599
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17761667"
FT CARBOHYD 1026
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17761667"
FT CARBOHYD 1036
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17761667"
SQ SEQUENCE 1408 AA; 155818 MW; E7C2EBF4BFC5C389 CRC64;
MLTLLEPPGA KRSPTVGNYN NRSAQHLLNL APEEDDLYET YEETEDYAEN EPTGNILTRS
FQFLDQYLVG QSSSADYNDR WKREFRARPS WCDADLCLQQ MNRGKATGNR YALYSRSLIQ
KLLFALGNTV HRNAWSIILA VSMIFAVCCY GLQYVHIETD IVKLWVAQGG RLDEELNFLP
NIKEAMRNVT GDSGPELPRE NGLGGGYQVL IQTPEYEGQD ALAAGPLLKH VEIMKHIASF
NVSVHGVDWS LSDICFKPAP PSVAADSAAS SLGDVIDKIV PCIWITPIDC FWEGSKALGP
HPSLPKSSLG PLGMLLSSLS DGDMIRWSDF DPIAVIDEIH RSFNLGSHYT FFERAGVSHG
YMDRPCIDPL DPECPPMAKN YFDVCPHIDR VREIAKKYGT ELEEEKKKDS GYSFFDFLGR
KKREAGDQPK MIHPAQPADS IPTIEDAVPA QVPVSTAPIP TTTTLSPEEA RAAEEKEKKQ
KARELKDYCK SYRKSAFEWL KKNKDKWPEV MSENMYPQNV DYAAEMTGGC SGFASNVLNW
PEDMILGNPR RAKKGGKLSG ADALQSVFLV ASPADVFLRF KQKPGRNSMK TGLDMDAWNE
TAAEQVLQAW QRNFTKSLYN HKANVDEDGN ERRTLHPLAS TSIADMLEEF CQFNYTIILA
GYALMLAYAI VTQARFDNCL PATESSMGLA LAGVLVVTFA SVAGLGLATW FGIEFNAATT
QIVPFLTLGI GVDNMFMLLH NYRDVVKLAG GHAEMAILMR ETGMSILCTS INNILSFLTG
TLLPIPALRS FCAQSSILLT FNFIAILTIY PAIISIDLRR KKAQRRDLVC CLYGDTREES
YSMISKPKIQ SKRIIGAPSE ASIMQQFDGI TQAQMASSDD PAPWSLHSFI RYYYIPFISK
PASKVAIIVG CCALLGASFI GMRQSTLGLE LGDVLPEHTA PAQFLRARDK YFSFYPMFAV
IKGPNIDYAH QQRQIDNYRQ SIGSSKYVIK NKNEEPSEKY WLGLMRDWLI SIQRGFDEEV
AKGSFNLTSG TVIGSNVSED ARLAHALMCS HGSLFGCAGR VGKIRLVDAS GIINSDGFYN
YLTAWFNVDH MMYYVSQASF FPTPPKWELS KNHTENFIPA AEPLAYSQIP FYLTGLTDTA
VIVDAIKDIR SVCERFTDQG LPNFPQGIAF TFWEQYLFLT GNLMQAISII TISVFCVISV
LLFNPWAALM VVCILGIMTC ELAGFMGLVG IKLNPVSAVT LITAVGIGVE FTVHVVVSFL
TALGTRSQRT SSAVDRVFVP VIHGSFSTLL GILMLGFSEF EFVVKYFFIV MTALICIGII
NGLILLPVLL SWFGPRREIS PTGGKTTLTL PPPLPKNANS SRSGGDDSDE DDEPSGLVMY
SRQAPPTTRT SGGNRGTVGN NTRRLPAV
