PTCA_LACLA
ID PTCA_LACLA Reviewed; 116 AA.
AC Q9CIE9;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=PTS system cellobiose-specific EIIA component {ECO:0000305};
GN Name=ptcA {ECO:0000303|PubMed:17909747};
GN ORFNames=L19292 {ECO:0000312|EMBL:AAK04512.1};
OS Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
OX NCBI_TaxID=272623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IL1403;
RX PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA Ehrlich S.D., Sorokin A.;
RT "The complete genome sequence of the lactic acid bacterium Lactococcus
RT lactis ssp. lactis IL1403.";
RL Genome Res. 11:731-753(2001).
RN [2]
RP FUNCTION, AND INDUCTION.
RC STRAIN=IL1403;
RX PubMed=17909747; DOI=10.1007/s00203-007-0308-8;
RA Kowalczyk M., Cocaign-Bousquet M., Loubiere P., Bardowski J.;
RT "Identification and functional characterisation of cellobiose and lactose
RT transport systems in Lactococcus lactis IL1403.";
RL Arch. Microbiol. 189:187-196(2008).
RN [3]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=IL1403;
RX PubMed=21262549; DOI=10.1016/j.ijfoodmicro.2010.12.011;
RA Aleksandrzak-Piekarczyk T., Polak J., Jezierska B., Renault P.,
RA Bardowski J.;
RT "Genetic characterization of the CcpA-dependent, cellobiose-specific PTS
RT system comprising CelB, PtcB and PtcA that transports lactose in
RT Lactococcus lactis IL1403.";
RL Int. J. Food Microbiol. 145:186-194(2011).
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC system (sugar PTS), a major carbohydrate active transport system,
CC catalyzes the phosphorylation of incoming sugar substrates
CC concomitantly with their translocation across the cell membrane (By
CC similarity). Involved in cellobiose transport with PtcB and CelB. This
CC system can also transport lactose (PubMed:17909747, PubMed:21262549).
CC {ECO:0000250|UniProtKB:P69791, ECO:0000269|PubMed:17909747,
CC ECO:0000269|PubMed:21262549}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:P23532};
CC Note=Binds 1 Mg(2+) ion per trimer. {ECO:0000250|UniProtKB:P23532};
CC -!- SUBUNIT: Homotrimer. {ECO:0000250|UniProtKB:P23532}.
CC -!- INDUCTION: Induced by growth on cellobiose. Negativelly controled by
CC the CcpA regulator (PubMed:17909747, PubMed:21262549). Also induced in
CC response to galactose (PubMed:21262549). {ECO:0000269|PubMed:17909747,
CC ECO:0000269|PubMed:21262549}.
CC -!- DOMAIN: The PTS EIIA type-3 domain is phosphorylated by phospho-HPr on
CC a histidyl residue. Then, it transfers the phosphoryl group to the PTS
CC EIIB type-3 domain. {ECO:0000255|PROSITE-ProRule:PRU00418}.
CC -!- DISRUPTION PHENOTYPE: Disruption of the gene abolishes growth on
CC cellobiose and lactose. {ECO:0000269|PubMed:21262549}.
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DR EMBL; AE005176; AAK04512.1; -; Genomic_DNA.
DR PIR; F86676; F86676.
DR RefSeq; NP_266570.1; NC_002662.1.
DR RefSeq; WP_003131552.1; NC_002662.1.
DR AlphaFoldDB; Q9CIE9; -.
DR SMR; Q9CIE9; -.
DR STRING; 272623.L19292; -.
DR TCDB; 4.A.3.2.4; the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family.
DR PaxDb; Q9CIE9; -.
DR EnsemblBacteria; AAK04512; AAK04512; L19292.
DR GeneID; 60356736; -.
DR GeneID; 66441385; -.
DR KEGG; lla:L19292; -.
DR PATRIC; fig|272623.7.peg.449; -.
DR eggNOG; COG1447; Bacteria.
DR HOGENOM; CLU_152490_0_0_9; -.
DR OMA; MEQSRMA; -.
DR Proteomes; UP000002196; Chromosome.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR CDD; cd00215; PTS_IIA_lac; 1.
DR InterPro; IPR003188; PTS_IIA_lac/cel.
DR InterPro; IPR036542; PTS_IIA_lac/cel_sf.
DR PANTHER; PTHR34382; PTHR34382; 1.
DR Pfam; PF02255; PTS_IIA; 1.
DR PIRSF; PIRSF000699; PTS_IILac_III; 1.
DR SUPFAM; SSF46973; SSF46973; 1.
DR PROSITE; PS51095; PTS_EIIA_TYPE_3; 1.
PE 2: Evidence at transcript level;
KW Magnesium; Metal-binding; Phosphoprotein; Phosphotransferase system;
KW Reference proteome; Sugar transport; Transferase; Transport.
FT CHAIN 1..116
FT /note="PTS system cellobiose-specific EIIA component"
FT /id="PRO_0000446883"
FT DOMAIN 11..109
FT /note="PTS EIIA type-3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00418"
FT ACT_SITE 85
FT /note="Tele-phosphohistidine intermediate"
FT /evidence="ECO:0000250|UniProtKB:P23532"
FT BINDING 88
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_note="ligand shared between all trimeric partners"
FT /evidence="ECO:0000250|UniProtKB:P23532"
FT MOD_RES 85
FT /note="Phosphohistidine; by HPr"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00418"
SQ SEQUENCE 116 AA; 12814 MW; 995F034417DA3DA0 CRC64;
MTDKYENPTS DDYMGVVMGI IMSGGNAKGL AFQAIQQAKA GEFAEAESSL NEASEQLREA
HDVQTDLLTR LAQGEKIGWN LYMVHAQDHL MNAITFKDLA VEVVGQEQRL QALENK
