PTCB_LACLA
ID PTCB_LACLA Reviewed; 108 AA.
AC Q9CIF0;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=PTS system cellobiose-specific EIIB component {ECO:0000305};
DE AltName: Full=Cellobiose-specific phosphotransferase enzyme IIB component {ECO:0000305};
DE EC=2.7.1.205 {ECO:0000305|PubMed:21262549};
GN Name=ptcB {ECO:0000303|PubMed:17909747};
GN ORFNames=L18872 {ECO:0000312|EMBL:AAK04511.1};
OS Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC Lactococcus.
OX NCBI_TaxID=272623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IL1403;
RX PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA Ehrlich S.D., Sorokin A.;
RT "The complete genome sequence of the lactic acid bacterium Lactococcus
RT lactis ssp. lactis IL1403.";
RL Genome Res. 11:731-753(2001).
RN [2]
RP FUNCTION, AND INDUCTION.
RC STRAIN=IL1403;
RX PubMed=17909747; DOI=10.1007/s00203-007-0308-8;
RA Kowalczyk M., Cocaign-Bousquet M., Loubiere P., Bardowski J.;
RT "Identification and functional characterisation of cellobiose and lactose
RT transport systems in Lactococcus lactis IL1403.";
RL Arch. Microbiol. 189:187-196(2008).
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=IL1403;
RX PubMed=21262549; DOI=10.1016/j.ijfoodmicro.2010.12.011;
RA Aleksandrzak-Piekarczyk T., Polak J., Jezierska B., Renault P.,
RA Bardowski J.;
RT "Genetic characterization of the CcpA-dependent, cellobiose-specific PTS
RT system comprising CelB, PtcB and PtcA that transports lactose in
RT Lactococcus lactis IL1403.";
RL Int. J. Food Microbiol. 145:186-194(2011).
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC system (sugar PTS), a major carbohydrate active transport system,
CC catalyzes the phosphorylation of incoming sugar substrates
CC concomitantly with their translocation across the cell membrane (By
CC similarity). Involved in cellobiose transport with PtcA and CelB. This
CC system can also transport lactose (PubMed:17909747, PubMed:21262549).
CC {ECO:0000250|UniProtKB:P69795, ECO:0000269|PubMed:17909747,
CC ECO:0000269|PubMed:21262549}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-cellobiose(out) + N(pros)-phospho-L-histidyl-[protein] = 6-
CC phospho-beta-D-glucosyl-(1->4)-D-glucose(in) + L-histidyl-[protein];
CC Xref=Rhea:RHEA:49292, Rhea:RHEA-COMP:9745, Rhea:RHEA-COMP:9746,
CC ChEBI:CHEBI:17057, ChEBI:CHEBI:29979, ChEBI:CHEBI:58312,
CC ChEBI:CHEBI:64837; EC=2.7.1.205;
CC Evidence={ECO:0000305|PubMed:21262549};
CC -!- INDUCTION: Induced by growth on cellobiose. Negativelly controled by
CC the CcpA regulator (PubMed:17909747, PubMed:21262549). Also induced in
CC response to galactose (PubMed:21262549). {ECO:0000269|PubMed:17909747,
CC ECO:0000269|PubMed:21262549}.
CC -!- DOMAIN: The PTS EIIB type-3 domain is phosphorylated by phospho-EIIA on
CC a cysteinyl residue. Then, it transfers the phosphoryl group to the
CC sugar substrate concomitantly with the sugar uptake processed by the
CC PTS EIIC type-3 domain. {ECO:0000255|PROSITE-ProRule:PRU00423}.
CC -!- DISRUPTION PHENOTYPE: Disruption of the gene abolishes growth on
CC lactose and severely impairs growth on cellobiose.
CC {ECO:0000269|PubMed:21262549}.
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DR EMBL; AE005176; AAK04511.1; -; Genomic_DNA.
DR PIR; E86676; E86676.
DR RefSeq; NP_266569.1; NC_002662.1.
DR RefSeq; WP_003131553.1; NC_002662.1.
DR AlphaFoldDB; Q9CIF0; -.
DR SMR; Q9CIF0; -.
DR STRING; 272623.L18872; -.
DR TCDB; 4.A.3.2.4; the pts lactose-n,n'-diacetylchitobiose-Beta-glucoside (lac) family.
DR PaxDb; Q9CIF0; -.
DR EnsemblBacteria; AAK04511; AAK04511; L18872.
DR GeneID; 60356853; -.
DR GeneID; 66441384; -.
DR KEGG; lla:L18872; -.
DR PATRIC; fig|272623.7.peg.448; -.
DR eggNOG; COG1440; Bacteria.
DR HOGENOM; CLU_147323_1_1_9; -.
DR OMA; YAIPEQN; -.
DR Proteomes; UP000002196; Chromosome.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR InterPro; IPR036095; PTS_EIIB-like_sf.
DR InterPro; IPR003501; PTS_EIIB_2/3.
DR InterPro; IPR013012; PTS_EIIB_3.
DR Pfam; PF02302; PTS_IIB; 1.
DR SUPFAM; SSF52794; SSF52794; 1.
DR PROSITE; PS51100; PTS_EIIB_TYPE_3; 1.
PE 1: Evidence at protein level;
KW Kinase; Phosphoprotein; Phosphotransferase system; Reference proteome;
KW Sugar transport; Transferase; Transport.
FT CHAIN 1..108
FT /note="PTS system cellobiose-specific EIIB component"
FT /id="PRO_5004324902"
FT DOMAIN 3..108
FT /note="PTS EIIB type-3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00423"
FT ACT_SITE 10
FT /note="Phosphocysteine intermediate"
FT /evidence="ECO:0000250|UniProtKB:P69795"
FT MOD_RES 10
FT /note="Phosphocysteine; by EIIA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00423"
SQ SEQUENCE 108 AA; 11310 MW; BD43C78C15D4FDEC CRC64;
MADKVIALAC AAGMSTSLLV SKMQKAAADN GKDYEIFAKS TADIDNMLAG TGSPKPDVLL
LGPQVAFMKG EVAKKAEIAG VPMDVIKMQD YGMMRGDKVL AAAENLMN
