PTCRA_MOUSE
ID PTCRA_MOUSE Reviewed; 199 AA.
AC P0C6B2;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 1.
DT 03-AUG-2022, entry version 83.
DE RecName: Full=Pre T-cell antigen receptor alpha;
DE Short=pT-alpha;
DE Short=pTa;
DE AltName: Full=gp33;
DE AltName: Full=pT-alpha-TCR;
DE Flags: Precursor;
GN Name=Ptcra;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBUNIT, AND TISSUE SPECIFICITY.
RX PubMed=7973703; DOI=10.1126/science.7973703;
RA Saint-Ruf C., Ungewiss K., Groettrup M., Bruno L., Fehling H.J.,
RA von Boehmer H.;
RT "Analysis and expression of a cloned pre-T cell receptor gene.";
RL Science 266:1208-1212(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
RC STRAIN=129/Ola, and 129/Sv; TISSUE=Liver;
RX PubMed=7672822; DOI=10.1007/bf00176445;
RA Fehling H.J., Laplace C., Mattei M.-G., Saint-Ruf C., von Boehmer H.;
RT "Genomic structure and chromosomal location of the mouse pre-T-cell
RT receptor alpha gene.";
RL Immunogenetics 42:275-281(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, AND TISSUE
RP SPECIFICITY.
RX PubMed=9647201;
RA Barber D.F., Passoni L., Wen L., Geng L., Hayday A.C.;
RT "The expression in vivo of a second isoform of pT alpha: implications for
RT the mechanism of pT alpha action.";
RL J. Immunol. 161:11-16(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: The pre-T-cell receptor complex (composed of PTCRA, TCRB and
CC the CD3 complex) regulates early T-cell development. Isoform 1 acts to
CC retain most TCRB intracellularly, while isoform 2 permits higher levels
CC of cell surface TCRB expression and facilitates signaling from the CD3-
CC TCRB complex. {ECO:0000269|PubMed:9647201}.
CC -!- SUBUNIT: Interacts with RHBDD1 (By similarity). Heterodimer with TCRB;
CC disulfide linked. This heterodimer assembles with CD3 proteins into a
CC signaling-competent pre-T-cell receptor complex. {ECO:0000250,
CC ECO:0000269|PubMed:7973703}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=pTalpha-a;
CC IsoId=P0C6B2-1; Sequence=Displayed;
CC Name=2; Synonyms=pTalpha-b;
CC IsoId=P0C6B2-2; Sequence=VSP_031447;
CC -!- TISSUE SPECIFICITY: Isoform 1 is expressed at higher levels than
CC isoform 2 in the thymus while only isoform 2 is expressed in polyclonal
CC beta-only cells. Isoform 1 shows a predominant expression in immature
CC thymocytes. {ECO:0000269|PubMed:7973703, ECO:0000269|PubMed:9647201}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA58963.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAI19063.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAI20747.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; U16958; AAA58963.1; ALT_INIT; mRNA.
DR EMBL; U27268; AAA84398.1; -; Genomic_DNA.
DR EMBL; BC119062; AAI19063.1; ALT_INIT; mRNA.
DR EMBL; BC120746; AAI20747.1; ALT_INIT; mRNA.
DR RefSeq; NP_035325.1; NM_011195.2.
DR AlphaFoldDB; P0C6B2; -.
DR SMR; P0C6B2; -.
DR STRING; 10090.ENSMUSP00000035683; -.
DR GlyGen; P0C6B2; 2 sites.
DR PhosphoSitePlus; P0C6B2; -.
DR PRIDE; P0C6B2; -.
DR ProteomicsDB; 291616; -. [P0C6B2-1]
DR ProteomicsDB; 291617; -. [P0C6B2-2]
DR GeneID; 19208; -.
DR KEGG; mmu:19208; -.
DR UCSC; uc008cuh.1; mouse. [P0C6B2-1]
DR CTD; 171558; -.
DR MGI; MGI:104857; Ptcra.
DR eggNOG; ENOG502SAGI; Eukaryota.
DR InParanoid; P0C6B2; -.
DR OrthoDB; 1389406at2759; -.
DR TreeFam; TF337868; -.
DR BioGRID-ORCS; 19208; 2 hits in 65 CRISPR screens.
DR PRO; PR:P0C6B2; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; P0C6B2; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0070244; P:negative regulation of thymocyte apoptotic process; IMP:MGI.
DR GO; GO:0070242; P:thymocyte apoptotic process; IMP:MGI.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR027834; PTCRA.
DR PANTHER; PTHR37866; PTHR37866; 1.
DR Pfam; PF15028; PTCRA; 1.
DR SUPFAM; SSF48726; SSF48726; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..16
FT /evidence="ECO:0000255"
FT CHAIN 17..199
FT /note="Pre T-cell antigen receptor alpha"
FT /id="PRO_0000319109"
FT TOPO_DOM 17..146
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 168..199
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 67
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 117
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 47..107
FT /evidence="ECO:0000250"
FT DISULFID 135
FT /note="Interchain (with TCRB)"
FT /evidence="ECO:0000250"
FT VAR_SEQ 20..126
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:9647201"
FT /id="VSP_031447"
SQ SEQUENCE 199 AA; 21491 MW; 5141E9361BF494BA CRC64;
MARTWLLLLL GVRCQALPSG IAGTPFPSLA PPITLLVDGR QHMLVVCLVL DAAPPGLDNP
VWFSAGNGSA LDAFTYGPSL APDGTWTSLA QLSLPSEELE AWEPLVCHTR PGAGGQNRST
HPLQLSGESS TARSCFPEPL GGTQRQVLWL SLLRLLLFKL LLLDVLLTCS HLRLHVLAGQ
HLQPPPSRKS LPPTHRIWT
