ID   PTEC_GEOSE              Reviewed;         451 AA.
AC   Q45400;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=PTS system cellobiose-specific EIIC component {ECO:0000303|PubMed:8407820};
DE   AltName: Full=Cellobiose permease IIC component {ECO:0000303|PubMed:8407820};
DE   AltName: Full=EIIC-Cel {ECO:0000303|PubMed:8407820};
GN   Name=celB {ECO:0000303|PubMed:8407820};
OS   Geobacillus stearothermophilus (Bacillus stearothermophilus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus.
OX   NCBI_TaxID=1422;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=XL-65-6;
RX   PubMed=8407820; DOI=10.1128/jb.175.20.6441-6450.1993;
RA   Lai X., Ingram L.O.;
RT   "Cloning and sequencing of a cellobiose phosphotransferase system operon
RT   from Bacillus stearothermophilus XL-65-6 and functional expression in
RT   Escherichia coli.";
RL   J. Bacteriol. 175:6441-6450(1993).
CC   -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC       system (sugar PTS), a major carbohydrate active transport system,
CC       catalyzes the phosphorylation of incoming sugar substrates
CC       concomitantly with their translocation across the cell membrane. The
CC       enzyme II CelABD PTS system is involved in cellobiose transport.
CC       {ECO:0000305|PubMed:8407820}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00428}; Multi-pass membrane protein {ECO:0000255|PROSITE-
CC       ProRule:PRU00428}.
CC   -!- DOMAIN: The EIIC type-3 domain forms the PTS system translocation
CC       channel and contains the specific substrate-binding site.
CC       {ECO:0000255|PROSITE-ProRule:PRU00428}.
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DR   EMBL; U07818; AAA17390.1; -; Unassigned_DNA.
DR   PIR; C49898; C49898.
DR   AlphaFoldDB; Q45400; -.
DR   SMR; Q45400; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR   InterPro; IPR003352; PTS_EIIC.
DR   InterPro; IPR004501; PTS_EIIC_3.
DR   InterPro; IPR004796; PTS_IIC_cello.
DR   Pfam; PF02378; PTS_EIIC; 1.
DR   PIRSF; PIRSF006351; PTS_EIIC-Cellobiose; 1.
DR   TIGRFAMs; TIGR00359; cello_pts_IIC; 1.
DR   TIGRFAMs; TIGR00410; lacE; 1.
DR   PROSITE; PS51105; PTS_EIIC_TYPE_3; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Phosphotransferase system; Sugar transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..451
FT                   /note="PTS system cellobiose-specific EIIC component"
FT                   /id="PRO_0000186483"
FT   TRANSMEM        31..51
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT   TRANSMEM        72..92
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT   TRANSMEM        104..124
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT   TRANSMEM        138..158
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT   TRANSMEM        187..207
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT   TRANSMEM        227..247
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT   TRANSMEM        250..270
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT   TRANSMEM        293..313
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT   TRANSMEM        347..367
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT   TRANSMEM        407..427
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
FT   DOMAIN          8..423
FT                   /note="PTS EIIC type-3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00428"
SQ   SEQUENCE   451 AA;  48805 MW;  18C3EBC0840D75AA CRC64;
     MDRFIRMLED RVMPVAGKIA EQRHLQAIRD GIILSMPLLI IGSLFLIVGF LPIPGYNEWM
     AKWFGEHWLD KLLYPVGATF DIMALVVSFG VAYRLAEKYK VDALSAGAIS LAAFLLATPY
     QVPFTPEGAK ETIMVSGGIP VQWVGSKGLF VAMILAIVST EIYRKIIQKN IVIKLPDGVP
     PAVARSFVAL IPGAAVLVVV WVARLILEMT PFESFHNIVS VLLNKPLSVL GGSVFGAIVA
     VLLVQLLWST GLHGAAIVGG VMGPIWLSLM DENRMVFQQN PNAELPNVIT QQFFDLWIYI
     GGSGATLALA LTMMFRARSR QLKSLGRLAI APGIFNINEP ITFGMPIVMN PLLIIPFILV
     PVVLVVVSYA AMATGLVAKP SGVAVPWTTP IVISGYLATG GKISGSILQI VNFFIAFAIY
     YPFFSIWDKQ KAAEEQADPT ISSGAGATHS L