ID   PTF1A_DANRE             Reviewed;         265 AA.
AC   Q7ZSX3;
DT   02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Pancreas transcription factor 1 subunit alpha;
DE   AltName: Full=Pancreas-specific transcription factor 1a;
DE   AltName: Full=bHLH transcription factor p48;
GN   Name=ptf1a; ORFNames=si:zc142h2.2, zgc:112216;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RX   PubMed=15183727; DOI=10.1016/j.ydbio.2004.02.023;
RA   Lin J.W., Biankin A.V., Horb M.E., Ghosh B., Prasad N.B., Yee N.S.,
RA   Pack M.A., Leach S.D.;
RT   "Differential requirement for ptf1a in endocrine and exocrine lineages of
RT   developing zebrafish pancreas.";
RL   Dev. Biol. 270:474-486(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Larva;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transcription factor implicated in the cell fate
CC       determination in various organs. Binds to the E-box consensus sequence
CC       5'-CANNTG-3'. Required for exocrine pancreatic development. Plays a
CC       central role in directing the differentiation of retinal progenitors
CC       towards horizontal and amacrine fates. {ECO:0000269|PubMed:15183727}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC   -!- DEVELOPMENTAL STAGE: Expression detected 24 hpf (hours post
CC       fertilization), in developing hindbrain, most abundant in rhombomeres 2
CC       and 3. Neural expression peaks at 48 hpf, at which point transcripts
CC       are found in the hindbrain, rhombic lip, and optic precursors. The
CC       exocrine pancreas represents a population of cells encircling the
CC       embryonic islet. At 72 hpf, expressed throughout the endodermal region
CC       corresponding to the exocrine pancreas. Expression is restricted to the
CC       exocrine pancreas following an early period of transient neural
CC       expression. Positive exocrine precursors and insulin-positive endocrine
CC       elements are both temporally and spatially segregated during
CC       embryogenesis. {ECO:0000269|PubMed:15183727}.
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DR   EMBL; AY245546; AAO92259.1; -; mRNA.
DR   EMBL; AL845362; CAD60845.1; -; Genomic_DNA.
DR   EMBL; BC093269; AAH93269.1; -; mRNA.
DR   RefSeq; NP_997524.1; NM_207641.2.
DR   AlphaFoldDB; Q7ZSX3; -.
DR   SMR; Q7ZSX3; -.
DR   STRING; 7955.ENSDARP00000011265; -.
DR   PaxDb; Q7ZSX3; -.
DR   Ensembl; ENSDART00000021987; ENSDARP00000011265; ENSDARG00000014479.
DR   GeneID; 368662; -.
DR   KEGG; dre:368662; -.
DR   CTD; 256297; -.
DR   ZFIN; ZDB-GENE-030616-579; ptf1a.
DR   eggNOG; KOG4029; Eukaryota.
DR   GeneTree; ENSGT00940000161000; -.
DR   HOGENOM; CLU_053709_0_0_1; -.
DR   InParanoid; Q7ZSX3; -.
DR   OMA; CYRDGAC; -.
DR   OrthoDB; 1269550at2759; -.
DR   PhylomeDB; Q7ZSX3; -.
DR   TreeFam; TF315153; -.
DR   PRO; PR:Q7ZSX3; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 2.
DR   Bgee; ENSDARG00000014479; Expressed in pancreatic system and 21 other tissues.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0005667; C:transcription regulator complex; ISS:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IGI:ZFIN.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IPI:ZFIN.
DR   GO; GO:0035881; P:amacrine cell differentiation; IMP:ZFIN.
DR   GO; GO:0009887; P:animal organ morphogenesis; IMP:ZFIN.
DR   GO; GO:0030154; P:cell differentiation; IMP:ZFIN.
DR   GO; GO:0032502; P:developmental process; IBA:GO_Central.
DR   GO; GO:0031017; P:exocrine pancreas development; IMP:ZFIN.
DR   GO; GO:0048699; P:generation of neurons; ISS:UniProtKB.
DR   GO; GO:0030902; P:hindbrain development; ISS:UniProtKB.
DR   GO; GO:0031016; P:pancreas development; IMP:ZFIN.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IGI:ZFIN.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; ISS:UniProtKB.
DR   GO; GO:0048384; P:retinoic acid receptor signaling pathway; ISS:UniProtKB.
DR   GO; GO:0009888; P:tissue development; ISS:UniProtKB.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..265
FT                   /note="Pancreas transcription factor 1 subunit alpha"
FT                   /id="PRO_0000233146"
FT   DOMAIN          115..167
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
SQ   SEQUENCE   265 AA;  30447 MW;  FBC91669CFBBC5D9 CRC64;
     MDTVLDPFTG LDSFSSSYFD DDDFFTDHSS RDHLDTDDFL DEDVDFLTNQ IQEYYKDSRI
     SQDGDYCDVG NFSFSSSSST FSYGCADSTS ELSPHRDGGL LKRRRRMRSE VEMQQLRQAA
     NVRERRRMQS INDAFEGLRS HIPTLPYEKR LSKVDTLRLA IGYINFLAEL VQSDMPIRNP
     HSDALNQPKK VIICHRGTRS PSPNDPDYGL PPLAGHSLSW TDEKQLKDQN IIRTAKVWTP
     EDPRKLHLKS SINNIENEPP FNFIS