PTF1A_RAT
ID PTF1A_RAT Reviewed; 326 AA.
AC Q64305;
DT 02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Pancreas transcription factor 1 subunit alpha;
DE AltName: Full=Pancreas-specific transcription factor 1a;
DE AltName: Full=bHLH transcription factor p48;
DE AltName: Full=p48 DNA-binding subunit of transcription factor PTF1;
DE Short=PTF1-p48;
GN Name=Ptf1a; Synonyms=Ptf1p48;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-18 AND 176-194, FUNCTION,
RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RC TISSUE=Pancreas;
RX PubMed=8861960; DOI=10.1002/j.1460-2075.1996.tb00806.x;
RA Krapp A., Knoefler M., Frutiger S., Hughes G.J., Hagenbuechle O.,
RA Wellauer P.K.;
RT "The p48 DNA-binding subunit of transcription factor PTF1 is a new exocrine
RT pancreas-specific basic helix-loop-helix protein.";
RL EMBO J. 15:4317-4329(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=Sprague-Dawley;
RX PubMed=8703005; DOI=10.1074/jbc.271.36.21993;
RA Knoefler M., Krapp A., Hagenbuechle O., Wellauer P.K.;
RT "Constitutive expression of the gene for the cell-specific p48 DNA-binding
RT subunit of pancreas transcription factor 1 in cultured cells is under
RT control of binding sites for transcription factors SP1 and alpha CBF.";
RL J. Biol. Chem. 271:21993-22002(1996).
RN [3]
RP FUNCTION IN PANCREAS DEVELOPMENT, AND TISSUE SPECIFICITY.
RX PubMed=10768861;
RA Adell T., Gomez-Cuadrado A., Skoudy A., Pettengill O.S., Longnecker D.S.,
RA Real F.X.;
RT "Role of the basic helix-loop-helix transcription factor p48 in the
RT differentiation phenotype of exocrine pancreas cancer cells.";
RL Cell Growth Differ. 11:137-147(2000).
CC -!- FUNCTION: Transcription factor implicated in the cell fate
CC determination in various organs. Binds to the E-box consensus sequence
CC 5'-CANNTG-3'. Plays a role in early and late pancreas development and
CC differentiation. Important for determining whether cells allocated to
CC the pancreatic buds continue towards pancreatic organogenesis or revert
CC back to duodenal fates. May be involved in the maintenance of exocrine
CC pancreas-specific gene expression including ELA1 and amylase. Required
CC for the formation of pancreatic acinar and ductal cells. Plays an
CC important role in cerebellar development. Directly regulated by FOXN4
CC and RORC during retinal development, FOXN4-PTF1A pathway plays a
CC central role in directing the differentiation of retinal progenitors
CC towards horizontal and amacrine fates. {ECO:0000269|PubMed:10768861,
CC ECO:0000269|PubMed:8703005, ECO:0000269|PubMed:8861960}.
CC -!- SUBUNIT: Component of the pancreas transcription factor 1 complex
CC (PTF1) which is composed of TCF3/p75, TCF12/p64 and PTF1A/p48. TCF3 is
CC responsible for the nuclear import of the p48/p64 complex. Interacts
CC with TCF3 and RBPSUH/RBP-Jkappa (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981,
CC ECO:0000269|PubMed:8861960}. Cytoplasm {ECO:0000269|PubMed:8861960}.
CC Note=In the cytoplasm loses its ability to form the PTF1 complex.
CC -!- TISSUE SPECIFICITY: Exocrine pancreas-specific. Expressed in azaserine-
CC induced pancreatic tumors (at protein level). Expressed in AR42J cells
CC but not in ARIP, DSL6A, or DSL6B cells. Down-regulation is associated
CC with the change of an azaserine-induced acinar cell carcinoma to a
CC ductal phenotype. {ECO:0000269|PubMed:10768861}.
CC -!- DEVELOPMENTAL STAGE: Expressed from day 12 of gestation in pancreatic
CC structures. {ECO:0000269|PubMed:8861960}.
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DR EMBL; X98170; CAA66851.1; -; mRNA.
DR EMBL; X98446; CAA67076.1; -; Genomic_DNA.
DR PIR; S71755; S71755.
DR RefSeq; NP_446416.1; NM_053964.1.
DR AlphaFoldDB; Q64305; -.
DR SMR; Q64305; -.
DR BioGRID; 250633; 1.
DR CORUM; Q64305; -.
DR STRING; 10116.ENSRNOP00000022727; -.
DR PaxDb; Q64305; -.
DR Ensembl; ENSRNOT00000022727; ENSRNOP00000022727; ENSRNOG00000016902.
DR GeneID; 117034; -.
DR KEGG; rno:117034; -.
DR UCSC; RGD:621709; rat.
DR CTD; 256297; -.
DR RGD; 621709; Ptf1a.
DR eggNOG; KOG4029; Eukaryota.
DR GeneTree; ENSGT00940000161000; -.
DR HOGENOM; CLU_053709_0_0_1; -.
DR InParanoid; Q64305; -.
DR OMA; CYRDGAC; -.
DR OrthoDB; 1269550at2759; -.
DR PhylomeDB; Q64305; -.
DR TreeFam; TF315153; -.
DR PRO; PR:Q64305; -.
DR Proteomes; UP000002494; Chromosome 17.
DR Bgee; ENSRNOG00000016902; Expressed in pancreas and 1 other tissue.
DR Genevisible; Q64305; RN.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; IDA:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; ISO:RGD.
DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:RGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0070888; F:E-box binding; ISO:RGD.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:MGI.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:RGD.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:RGD.
DR GO; GO:0035881; P:amacrine cell differentiation; ISS:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; ISO:RGD.
DR GO; GO:0045165; P:cell fate commitment; ISO:RGD.
DR GO; GO:0021549; P:cerebellum development; ISS:UniProtKB.
DR GO; GO:0032502; P:developmental process; IBA:GO_Central.
DR GO; GO:0031017; P:exocrine pancreas development; IDA:UniProtKB.
DR GO; GO:0048699; P:generation of neurons; ISS:HGNC.
DR GO; GO:0030902; P:hindbrain development; ISS:HGNC.
DR GO; GO:0048663; P:neuron fate commitment; ISO:RGD.
DR GO; GO:0031016; P:pancreas development; ISO:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISS:HGNC.
DR GO; GO:0061074; P:regulation of neural retina development; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0010842; P:retina layer formation; ISS:UniProtKB.
DR GO; GO:0060042; P:retina morphogenesis in camera-type eye; ISO:RGD.
DR GO; GO:0048384; P:retinoic acid receptor signaling pathway; ISS:HGNC.
DR GO; GO:0009888; P:tissue development; ISS:UniProtKB.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IEA:Ensembl.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Differentiation;
KW Direct protein sequencing; DNA-binding; Neurogenesis; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..326
FT /note="Pancreas transcription factor 1 subunit alpha"
FT /id="PRO_0000233145"
FT DOMAIN 162..214
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 229..249
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 304..326
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 326 AA; 35305 MW; E06D0132390E429F CRC64;
MDAVLLEHFP GALDTFPSSY FDEEDFFTDQ SSRDPLEDGD ELLGDEQAEV EFLSHQLHEY
CYRDGACLLL QPAPSAAPHA LAPPPLGDPG EPEDSGSYCC DAGAPLGAFP YSPGSPPSCL
AYPCTAVLSP GTRLRGLNGA AAAAAAAARR RRRVRSEAEL QQLRQAANVR ERRRMQSIND
AFEGLRSHIP TLPYEKRLSK VDTLRLAIGY INFLSELVQA DLPLRGSGTG GCGGPGGSRH
LGGDSPGNQA QKVIICHRGT RSPSPSDPDY GLPPLAGHSL SWADEKQLKE QNIIRTAKVW
TPEDPRKLNS KSFDNIENEP PFEFVS
