PTF2_ARATH
ID PTF2_ARATH Reviewed; 527 AA.
AC O81787;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Plant-specific TFIIB-related protein PTF2 {ECO:0000305};
DE AltName: Full=Protein POLLEN-EXPRESSED TRANSCRIPTION FACTOR 2 {ECO:0000303|PubMed:23713077};
DE AltName: Full=TFIIB-related protein PTF2 {ECO:0000305};
GN Name=PTF2 {ECO:0000303|PubMed:23713077};
GN OrderedLocusNames=At4g35540 {ECO:0000312|Araport:AT4G35540};
GN ORFNames=F8D20.50 {ECO:0000312|EMBL:CAA20024.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, SUBUNIT, INTERACTION WITH TBP2, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=23713077; DOI=10.1093/mp/sst083;
RA Niu Q.K., Liang Y., Zhou J.J., Dou X.Y., Gao S.C., Chen L.Q., Zhang X.Q.,
RA Ye D.;
RT "Pollen-expressed transcription factor 2 encodes a novel plant-specific
RT TFIIB-related protein that is required for pollen germination and
RT embryogenesis in Arabidopsis.";
RL Mol. Plant 6:1091-1108(2013).
CC -!- FUNCTION: Plant-specific TFIIB-related protein that plays important
CC roles in pollen germination and embryogenesis, possibly by regulating
CC gene expression through interaction with TBP2 and the subunits of RNA
CC polymerases. Binds double-stranded DNA in vitro.
CC {ECO:0000269|PubMed:23713077}.
CC -!- SUBUNIT: Can form homodimer (PubMed:23713077). Interacts with TBP2
CC (PubMed:23713077). {ECO:0000269|PubMed:23713077}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23713077}.
CC -!- TISSUE SPECIFICITY: Expressed in shoot apical meristems, root tips,
CC primordia of lateral roots, inflorescences, developing pollen grains
CC and embryos. {ECO:0000269|PubMed:23713077}.
CC -!- DEVELOPMENTAL STAGE: In developing pollen grains, expressed in the
CC vegetative nuclei at the early binucleate stage and during the second
CC pollen mitosis. Expression significantly decreases at the late
CC trinucleate developmental stage. Not expressed in released mature
CC pollen grains, germinating pollen grains and pollen tubes.
CC {ECO:0000269|PubMed:23713077}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality and aborted seed when
CC homozygous. {ECO:0000269|PubMed:23713077}.
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DR EMBL; AL031135; CAA20024.1; -; Genomic_DNA.
DR EMBL; AL161587; CAB80270.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE86528.1; -; Genomic_DNA.
DR PIR; T04659; T04659.
DR RefSeq; NP_195279.1; NM_119719.2.
DR AlphaFoldDB; O81787; -.
DR SMR; O81787; -.
DR STRING; 3702.AT4G35540.1; -.
DR PaxDb; O81787; -.
DR PRIDE; O81787; -.
DR ProteomicsDB; 248697; -.
DR EnsemblPlants; AT4G35540.1; AT4G35540.1; AT4G35540.
DR GeneID; 829706; -.
DR Gramene; AT4G35540.1; AT4G35540.1; AT4G35540.
DR KEGG; ath:AT4G35540; -.
DR Araport; AT4G35540; -.
DR TAIR; locus:2127953; AT4G35540.
DR eggNOG; KOG1598; Eukaryota.
DR HOGENOM; CLU_039109_0_0_1; -.
DR InParanoid; O81787; -.
DR OMA; GWTKDMV; -.
DR OrthoDB; 967764at2759; -.
DR PhylomeDB; O81787; -.
DR PRO; PR:O81787; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O81787; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0097550; C:transcription preinitiation complex; IBA:GO_Central.
DR GO; GO:0003690; F:double-stranded DNA binding; IDA:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0042803; F:protein homodimerization activity; IPI:UniProtKB.
DR GO; GO:0070063; F:RNA polymerase binding; IDA:TAIR.
DR GO; GO:0017025; F:TBP-class protein binding; IBA:GO_Central.
DR GO; GO:0001093; F:TFIIB-class transcription factor binding; IDA:TAIR.
DR GO; GO:0006352; P:DNA-templated transcription, initiation; IBA:GO_Central.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0009846; P:pollen germination; IMP:TAIR.
DR GO; GO:0070897; P:transcription preinitiation complex assembly; IEA:InterPro.
DR InterPro; IPR036915; Cyclin-like_sf.
DR InterPro; IPR000812; TFIIB.
DR InterPro; IPR013137; Znf_TFIIB.
DR PANTHER; PTHR11618; PTHR11618; 1.
DR Pfam; PF08271; TF_Zn_Ribbon; 1.
DR SUPFAM; SSF47954; SSF47954; 1.
PE 1: Evidence at protein level;
KW Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..527
FT /note="Plant-specific TFIIB-related protein PTF2"
FT /id="PRO_0000436816"
FT ZN_FING 1..30
FT /note="TFIIB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00469"
SQ SEQUENCE 527 AA; 59591 MW; 587D1A16E123098E CRC64;
MRCKRCNGSN FERDEDTGNS YCGGCGTLRE YDNYEAQLGG IRGPQGTYIR VGTIGRGSVL
DYKDKKIYEA NNLIEETTER LNLGNKTEVI KSMISKLTDG EFGQGEWFPI LIGACCYAVV
REEGKGVLSM EEVAYEVGCD LHQLGPMIKR VVDHLDLELR EFDLVGLFTK TVTNSPRLTD
VDRDKKEKII KQGTFLMNCA LKWFLSTGRR PMPLVVAVLA FVVQVNGVKV KIDDLAKDAS
VSLTTCKTRY KELSEKLVKV AEEVGLPWAK DVTVKNVLKH SGTLFALMEA KSMKKRKQGT
GKELVRTDGF CVEDLVMDCL SKESMYCYDD DARQDTMSRY FDVEGERQLS LCNYDDNISE
NQLSTKYNEF EDRVCGGTLA KRSQGSSQSM WQRRSVFGMV STENWWKGKS ELSKRLLLKD
LLEKDVGLEA LPPSYIKGCV AVERRREKIK AAKLRINAIQ HPSDNVSEGA LSLELEHSKK
KRKKGSEIDW EDLVIQTLVL HNVNEEEIEK GHYKTLLDLH VFNSGEV
