PTFA_HALVD
ID PTFA_HALVD Reviewed; 154 AA.
AC D4GYE4;
DT 14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 1.
DT 03-AUG-2022, entry version 54.
DE RecName: Full=PTS system fructose-specific EIIA component {ECO:0000303|PubMed:22493022};
DE AltName: Full=EIIA-Fru {ECO:0000303|PubMed:22493022};
DE AltName: Full=Fructose-specific phosphotransferase enzyme IIA component {ECO:0000303|PubMed:22493022};
GN Name=ptfA {ECO:0000303|PubMed:20333302}; Synonyms=fruA2;
GN OrderedLocusNames=HVO_1498; ORFNames=C498_11241;
OS Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Haloferacaceae; Haloferax.
OX NCBI_TaxID=309800;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RX PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT "The complete genome sequence of Haloferax volcanii DS2, a model
RT archaeon.";
RL PLoS ONE 5:E9605-E9605(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RX PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT strategies for static and dynamic osmo-response.";
RL PLoS Genet. 10:E1004784-E1004784(2014).
RN [3]
RP IDENTIFICATION, FUNCTION, AND INDUCTION.
RC STRAIN=DS2 / DS70;
RX PubMed=22493022; DOI=10.1128/jb.00200-12;
RA Pickl A., Johnsen U., Schoenheit P.;
RT "Fructose degradation in the haloarchaeon Haloferax volcanii involves a
RT bacterial type phosphoenolpyruvate-dependent phosphotransferase system,
RT fructose-1-phosphate kinase, and class II fructose-1,6-bisphosphate
RT aldolase.";
RL J. Bacteriol. 194:3088-3097(2012).
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC system (sugar PTS), a major carbohydrate active transport system,
CC catalyzes the phosphorylation of incoming sugar substrates
CC concomitantly with their translocation across the cell membrane. The
CC enzyme II PtfABC PTS system is involved in fructose transport.
CC {ECO:0000269|PubMed:22493022}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Expression is highly up-regulated in presence of fructose.
CC {ECO:0000269|PubMed:22493022}.
CC -!- DOMAIN: The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on
CC a histidyl residue. Then, it transfers the phosphoryl group to the PTS
CC EIIB type-2 domain. {ECO:0000255|PROSITE-ProRule:PRU00417}.
CC -!- MISCELLANEOUS: PTS-type transport systems are very rare in archaea.
CC {ECO:0000305}.
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DR EMBL; CP001956; ADE03077.1; -; Genomic_DNA.
DR EMBL; AOHU01000090; ELY28264.1; -; Genomic_DNA.
DR RefSeq; WP_004043437.1; NZ_AOHU01000090.1.
DR AlphaFoldDB; D4GYE4; -.
DR SMR; D4GYE4; -.
DR STRING; 309800.C498_11241; -.
DR TCDB; 4.A.2.1.15; the pts fructose-mannitol (fru) family.
DR EnsemblBacteria; ADE03077; ADE03077; HVO_1498.
DR EnsemblBacteria; ELY28264; ELY28264; C498_11241.
DR GeneID; 8925300; -.
DR KEGG; hvo:HVO_1498; -.
DR PATRIC; fig|309800.29.peg.2142; -.
DR eggNOG; arCOG10195; Archaea.
DR HOGENOM; CLU_072531_5_0_2; -.
DR OMA; FDAPDEQ; -.
DR OrthoDB; 91552at2157; -.
DR Proteomes; UP000008243; Chromosome.
DR Proteomes; UP000011532; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:InterPro.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd00211; PTS_IIA_fru; 1.
DR Gene3D; 3.40.930.10; -; 1.
DR InterPro; IPR016152; PTrfase/Anion_transptr.
DR InterPro; IPR002178; PTS_EIIA_type-2_dom.
DR InterPro; IPR004715; PTS_IIA_fruc.
DR Pfam; PF00359; PTS_EIIA_2; 1.
DR SUPFAM; SSF55804; SSF55804; 1.
DR TIGRFAMs; TIGR00848; fruA; 1.
DR PROSITE; PS51094; PTS_EIIA_TYPE_2; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Kinase; Phosphoprotein; Phosphotransferase system;
KW Reference proteome; Sugar transport; Transferase; Transport.
FT CHAIN 1..154
FT /note="PTS system fructose-specific EIIA component"
FT /id="PRO_0000428982"
FT DOMAIN 8..152
FT /note="PTS EIIA type-2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00417"
FT ACT_SITE 70
FT /note="Tele-phosphohistidine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00417"
FT MOD_RES 70
FT /note="Phosphohistidine; by HPr"
FT /evidence="ECO:0000305"
SQ SEQUENCE 154 AA; 16453 MW; 99184BF8743BD211 CRC64;
MDVTDISTIT PLELISLEEP PATKEGAIEF LLDLAVDAGR VDDRDAALDA LLEREGEATT
GVGFGIGIPH AKTDAVSKPT VAFARSAEGI DFDAMDDKPA KLLFMILVPA AGGEDHLQIL
SALSRSLMHE DVREKLLEAE SKQTVQDVLA EVVE
