PTFD_BACSU
ID PTFD_BACSU Reviewed; 275 AA.
AC P26382;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Fructose permease IID component;
DE AltName: Full=EIID-Fru;
DE AltName: Full=PTS system fructose-specific EIID component;
DE AltName: Full=p30;
GN Name=levG; OrderedLocusNames=BSU27040;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=2117666; DOI=10.1016/0022-2836(90)90284-s;
RA Martin-Verstraete I., Debarbouille M., Klier A., Rapoport G.;
RT "Levanase operon of Bacillus subtilis includes a fructose-specific
RT phosphotransferase system regulating the expression of the operon.";
RL J. Mol. Biol. 214:657-671(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9141695; DOI=10.1099/00221287-143-4-1321;
RA Parro V., San Roman M., Galindo I., Purnelle B., Bolotin A., Sorokin A.,
RA Mellado R.P.;
RT "A 23911 bp region of the Bacillus subtilis genome comprising genes located
RT upstream and downstream of the lev operon.";
RL Microbiology 143:1321-1326(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC system (PTS), a major carbohydrate active -transport system, catalyzes
CC the phosphorylation of incoming sugar substrates concomitant with their
CC translocation across the cell membrane. This system is involved in
CC fructose transport.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DOMAIN: The EIID domain, with its homologous EIIC domain, forms the PTS
CC system translocation channel and contains part of its specific
CC substrate-binding site.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X56098; CAA39580.1; -; Genomic_DNA.
DR EMBL; X92868; CAA63464.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14646.1; -; Genomic_DNA.
DR PIR; S11401; S11401.
DR RefSeq; NP_390582.1; NC_000964.3.
DR RefSeq; WP_003229834.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; P26382; -.
DR SMR; P26382; -.
DR STRING; 224308.BSU27040; -.
DR TCDB; 4.A.6.1.2; the pts mannose-fructose-sorbose (man) family.
DR PaxDb; P26382; -.
DR PRIDE; P26382; -.
DR EnsemblBacteria; CAB14646; CAB14646; BSU_27040.
DR GeneID; 937595; -.
DR KEGG; bsu:BSU27040; -.
DR PATRIC; fig|224308.179.peg.2937; -.
DR eggNOG; COG3716; Bacteria.
DR InParanoid; P26382; -.
DR OMA; GDSFFWH; -.
DR PhylomeDB; P26382; -.
DR BioCyc; BSUB:BSU27040-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IBA:GO_Central.
DR InterPro; IPR004704; PTS_IID_man.
DR Pfam; PF03613; EIID-AGA; 1.
DR TIGRFAMs; TIGR00828; EIID-AGA; 1.
DR PROSITE; PS51108; PTS_EIID; 1.
PE 4: Predicted;
KW Cell membrane; Membrane; Phosphotransferase system; Reference proteome;
KW Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..275
FT /note="Fructose permease IID component"
FT /id="PRO_0000186522"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00431"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00431"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00431"
FT TRANSMEM 227..247
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00431"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00431"
FT DOMAIN 5..274
FT /note="PTS EIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00431"
SQ SEQUENCE 275 AA; 30087 MW; E974231F61A7D703 CRC64;
MEKEKRLTKK EIFSMFIRSN FLLGSFNFER VQAMGYCYVM IPAIKKLYGP GAKRNEALQR
HLEWFNTHPW LTAPIFGVTA AMEEEMANNK GIDGKAISGM KIGLMGPIAG VGDPIFWGTI
RPVLAALGAS LALGGNIAGP LLFFFLLNAI RLSTKYYGLK YGYVKGMEIL QDLAGNRIQK
LTEGASILGL FVMGALVSKW TTINIPIVVS RIKDESGKVD VQTVQNVLDS IMPGALPLGL
TLLVAWMLRK GVNPLLIICG IFVIGILGYW AGFLA
