PTFLA_ECOLI
ID PTFLA_ECOLI Reviewed; 148 AA.
AC P32155; P76776; Q2M8J7;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=PTS system fructose-like EIIA component {ECO:0000305|PubMed:8019415};
DE AltName: Full=Fructose-like phosphotransferase enzyme IIA component {ECO:0000305|PubMed:8019415};
GN Name=frvA {ECO:0000303|PubMed:8019415}; Synonyms=yiiK;
GN OrderedLocusNames=b3900, JW3871;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8396120; DOI=10.1128/jb.175.17.5585-5594.1993;
RA Moralejo P., Egan S.M., Hidalgo E.F., Aguilar J.;
RT "Sequencing and characterization of a gene cluster encoding the enzymes for
RT L-rhamnose metabolism in Escherichia coli.";
RL J. Bacteriol. 175:5585-5594(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8346018; DOI=10.1093/nar/21.15.3391;
RA Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome. III. DNA sequence of the region
RT from 87.2 to 89.2 minutes.";
RL Nucleic Acids Res. 21:3391-3398(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO 81
RP AND 104-108.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP FUNCTION, AND DISCUSSION OF SEQUENCE.
RX PubMed=8019415; DOI=10.1002/pro.5560030309;
RA Reizer J., Michotey V., Reizer A., Saier M.H. Jr.;
RT "Novel phosphotransferase system genes revealed by bacterial genome
RT analysis: unique, putative fructose- and glucoside-specific systems.";
RL Protein Sci. 3:440-450(1994).
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC system (sugar PTS), a major carbohydrate active transport system,
CC catalyzes the phosphorylation of incoming sugar substrates
CC concomitantly with their translocation across the cell membrane. The
CC enzyme II FrvAB PTS system is involved in fructose transport.
CC {ECO:0000305|PubMed:8019415}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- DOMAIN: The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on
CC a histidyl residue. Then, it transfers the phosphoryl group to the PTS
CC EIIB type-2 domain. {ECO:0000255|PROSITE-ProRule:PRU00417}.
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DR EMBL; X60472; CAA43004.1; -; Genomic_DNA.
DR EMBL; L19201; AAB03033.2; -; Genomic_DNA.
DR EMBL; U00096; AAC76882.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77409.1; -; Genomic_DNA.
DR PIR; D48649; D48649.
DR RefSeq; NP_418336.1; NC_000913.3.
DR RefSeq; WP_000729595.1; NZ_SSZK01000026.1.
DR AlphaFoldDB; P32155; -.
DR SMR; P32155; -.
DR BioGRID; 4261599; 7.
DR ComplexPortal; CPX-5979; Frv fructose-like enzyme II complex.
DR IntAct; P32155; 1.
DR STRING; 511145.b3900; -.
DR TCDB; 4.A.2.1.25; the pts fructose-mannitol (fru) family.
DR PaxDb; P32155; -.
DR EnsemblBacteria; AAC76882; AAC76882; b3900.
DR EnsemblBacteria; BAE77409; BAE77409; BAE77409.
DR GeneID; 66672191; -.
DR GeneID; 948391; -.
DR KEGG; ecj:JW3871; -.
DR KEGG; eco:b3900; -.
DR PATRIC; fig|1411691.4.peg.2807; -.
DR EchoBASE; EB1810; -.
DR eggNOG; COG1762; Bacteria.
DR HOGENOM; CLU_072531_5_1_6; -.
DR OMA; PIECSFI; -.
DR PhylomeDB; P32155; -.
DR BioCyc; EcoCyc:FRVA-MON; -.
DR BioCyc; MetaCyc:FRVA-MON; -.
DR PRO; PR:P32155; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:1902495; C:transmembrane transporter complex; IC:ComplexPortal.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; ISA:EcoCyc.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd00211; PTS_IIA_fru; 1.
DR Gene3D; 3.40.930.10; -; 1.
DR InterPro; IPR016152; PTrfase/Anion_transptr.
DR InterPro; IPR002178; PTS_EIIA_type-2_dom.
DR InterPro; IPR004715; PTS_IIA_fruc.
DR Pfam; PF00359; PTS_EIIA_2; 1.
DR SUPFAM; SSF55804; SSF55804; 1.
DR TIGRFAMs; TIGR00848; fruA; 1.
DR PROSITE; PS51094; PTS_EIIA_TYPE_2; 1.
DR PROSITE; PS00372; PTS_EIIA_TYPE_2_HIS; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Kinase; Phosphoprotein; Phosphotransferase system;
KW Reference proteome; Sugar transport; Transferase; Transport.
FT CHAIN 1..148
FT /note="PTS system fructose-like EIIA component"
FT /id="PRO_0000186500"
FT DOMAIN 2..145
FT /note="PTS EIIA type-2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00417"
FT ACT_SITE 64
FT /note="Tele-phosphohistidine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00417"
FT MOD_RES 64
FT /note="Phosphohistidine; by HPr"
FT /evidence="ECO:0000305"
FT CONFLICT 81
FT /note="A -> G (in Ref. 2; AAB03033)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 148 AA; 16094 MW; 04AE87B9084BA1C6 CRC64;
MAALTASCID LNIQGNGAYS VLKQLATIAL QNGFITDSHQ FLQTLLLREK MHSTGFGSGV
AVPHGKSACV KQPFVLFARK AQAIDWKASD GEDVNCWICL GVPQSGEEDQ VKIIGTLCRK
IIHKEFIHQL QQGDTDQVLA LLNQTLSS
