PTH1R_MOUSE
ID PTH1R_MOUSE Reviewed; 591 AA.
AC P41593; Q62119; Q80WU8;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 178.
DE RecName: Full=Parathyroid hormone/parathyroid hormone-related peptide receptor;
DE AltName: Full=PTH/PTHrP type I receptor;
DE Short=PTH/PTHr receptor;
DE AltName: Full=Parathyroid hormone 1 receptor;
DE Short=PTH1 receptor;
DE Flags: Precursor;
GN Name=Pth1r; Synonyms=Pthr, Pthr1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=C3H/HEHA;
RX PubMed=7524627; DOI=10.1016/0925-4773(94)90093-0;
RA Karperien M., van Dijk T.B., Hoeijmakers T., Cremers F., Abou-Samra A.-B.,
RA Boonstra J., de Laat S.W., Defize L.H.K.;
RT "Expression pattern of parathyroid hormone/parathyroid hormone related
RT peptide receptor mRNA in mouse postimplantation embryos indicates
RT involvement in multiple developmental processes.";
RL Mech. Dev. 47:29-42(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=BALB/cJ;
RX PubMed=8197183; DOI=10.1073/pnas.91.11.5051;
RA McCuaig K.A., Clarke J.C., White J.H.;
RT "Molecular cloning of the gene encoding the mouse parathyroid
RT hormone/parathyroid hormone-related peptide receptor.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:5051-5055(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Receptor for parathyroid hormone and for parathyroid hormone-
CC related peptide (PubMed:8197183). The activity of this receptor is
CC mediated by G proteins which activate adenylyl cyclase and also a
CC phosphatidylinositol-calcium second messenger system.
CC {ECO:0000250|UniProtKB:Q03431, ECO:0000269|PubMed:8197183}.
CC -!- SUBUNIT: Interacts (via N-terminal extracellular domain) with PTHLH and
CC PTH (PubMed:8197183). Homodimer in the absence of bound ligand. Peptide
CC hormone binding leads to dissociation of the homodimer.
CC {ECO:0000250|UniProtKB:Q03431, ECO:0000305|PubMed:8197183}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:8197183};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Detected in kidney. {ECO:0000269|PubMed:8197183}.
CC -!- PTM: N-glycosylated. {ECO:0000250|UniProtKB:Q03431}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 2 family.
CC {ECO:0000305}.
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DR EMBL; X78936; CAA55536.1; -; mRNA.
DR EMBL; L34611; AAA40011.1; -; Genomic_DNA.
DR EMBL; L34608; AAA40011.1; JOINED; Genomic_DNA.
DR EMBL; L34607; AAA40011.1; JOINED; Genomic_DNA.
DR EMBL; L34609; AAA40011.1; JOINED; Genomic_DNA.
DR EMBL; L34610; AAA40011.1; JOINED; Genomic_DNA.
DR EMBL; BC051981; AAH51981.1; -; mRNA.
DR CCDS; CCDS52940.1; -.
DR PIR; I59297; I59297.
DR PIR; S44203; S44203.
DR RefSeq; NP_001077404.1; NM_001083935.1.
DR RefSeq; NP_001077405.1; NM_001083936.1.
DR RefSeq; NP_035329.2; NM_011199.2.
DR AlphaFoldDB; P41593; -.
DR SMR; P41593; -.
DR BioGRID; 202466; 1.
DR IntAct; P41593; 1.
DR STRING; 10090.ENSMUSP00000006005; -.
DR ChEMBL; CHEMBL1075300; -.
DR GlyGen; P41593; 4 sites.
DR iPTMnet; P41593; -.
DR PhosphoSitePlus; P41593; -.
DR MaxQB; P41593; -.
DR PaxDb; P41593; -.
DR PRIDE; P41593; -.
DR ProteomicsDB; 302008; -.
DR Antibodypedia; 1960; 424 antibodies from 39 providers.
DR DNASU; 19228; -.
DR Ensembl; ENSMUST00000006005; ENSMUSP00000006005; ENSMUSG00000032492.
DR Ensembl; ENSMUST00000166716; ENSMUSP00000132064; ENSMUSG00000032492.
DR Ensembl; ENSMUST00000198865; ENSMUSP00000143298; ENSMUSG00000032492.
DR GeneID; 19228; -.
DR KEGG; mmu:19228; -.
DR UCSC; uc009ruo.1; mouse.
DR CTD; 5745; -.
DR MGI; MGI:97801; Pth1r.
DR VEuPathDB; HostDB:ENSMUSG00000032492; -.
DR eggNOG; KOG4564; Eukaryota.
DR GeneTree; ENSGT00940000158574; -.
DR InParanoid; P41593; -.
DR OMA; ESDKGWM; -.
DR OrthoDB; 651627at2759; -.
DR PhylomeDB; P41593; -.
DR TreeFam; TF315710; -.
DR Reactome; R-MMU-373080; Class B/2 (Secretin family receptors).
DR Reactome; R-MMU-418555; G alpha (s) signalling events.
DR BioGRID-ORCS; 19228; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Pth1r; mouse.
DR PRO; PR:P41593; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; P41593; protein.
DR Bgee; ENSMUSG00000032492; Expressed in right kidney and 266 other tissues.
DR ExpressionAtlas; P41593; baseline and differential.
DR Genevisible; P41593; MM.
DR GO; GO:0016324; C:apical plasma membrane; ISO:MGI.
DR GO; GO:0016323; C:basolateral plasma membrane; ISO:MGI.
DR GO; GO:0031526; C:brush border membrane; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0043235; C:receptor complex; ISO:MGI.
DR GO; GO:0008528; F:G protein-coupled peptide receptor activity; IBA:GO_Central.
DR GO; GO:0004991; F:parathyroid hormone receptor activity; IMP:MGI.
DR GO; GO:0017046; F:peptide hormone binding; ISS:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0043621; F:protein self-association; ISO:MGI.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IMP:MGI.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; ISO:MGI.
DR GO; GO:0030282; P:bone mineralization; IMP:MGI.
DR GO; GO:0045453; P:bone resorption; IMP:MGI.
DR GO; GO:0048469; P:cell maturation; IMP:MGI.
DR GO; GO:0008283; P:cell population proliferation; IMP:MGI.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IEA:InterPro.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IMP:MGI.
DR GO; GO:0002062; P:chondrocyte differentiation; IMP:MGI.
DR GO; GO:0007187; P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; ISO:MGI.
DR GO; GO:0001701; P:in utero embryonic development; IMP:MGI.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IMP:MGI.
DR GO; GO:0001503; P:ossification; IMP:MGI.
DR GO; GO:0002076; P:osteoblast development; IMP:MGI.
DR GO; GO:0007200; P:phospholipase C-activating G protein-coupled receptor signaling pathway; IMP:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IGI:MGI.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; ISO:MGI.
DR GO; GO:0060732; P:positive regulation of inositol phosphate biosynthetic process; ISO:MGI.
DR GO; GO:0001501; P:skeletal system development; IMP:MGI.
DR Gene3D; 4.10.1240.10; -; 1.
DR InterPro; IPR017981; GPCR_2-like.
DR InterPro; IPR036445; GPCR_2_extracell_dom_sf.
DR InterPro; IPR001879; GPCR_2_extracellular_dom.
DR InterPro; IPR002170; GPCR_2_parathyroid_rcpt.
DR InterPro; IPR000832; GPCR_2_secretin-like.
DR InterPro; IPR017983; GPCR_2_secretin-like_CS.
DR PANTHER; PTHR45620:SF27; PTHR45620:SF27; 1.
DR Pfam; PF00002; 7tm_2; 1.
DR Pfam; PF02793; HRM; 1.
DR PRINTS; PR00249; GPCRSECRETIN.
DR PRINTS; PR00393; PTRHORMONER.
DR SMART; SM00008; HormR; 1.
DR SUPFAM; SSF111418; SSF111418; 1.
DR PROSITE; PS00649; G_PROTEIN_RECEP_F2_1; 1.
DR PROSITE; PS00650; G_PROTEIN_RECEP_F2_2; 1.
DR PROSITE; PS50227; G_PROTEIN_RECEP_F2_3; 1.
DR PROSITE; PS50261; G_PROTEIN_RECEP_F2_4; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Signal; Transducer; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..591
FT /note="Parathyroid hormone/parathyroid hormone-related
FT peptide receptor"
FT /id="PRO_0000012846"
FT TOPO_DOM 27..188
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..212
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 213..219
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..239
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 240..282
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..306
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 307..320
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 321..342
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 343..361
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 362..382
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 383..409
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 410..428
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 429..440
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 441..463
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 464..591
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 67..104
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 474..477
FT /note="Important for interaction with G proteins"
FT /evidence="ECO:0000250"
FT COMPBIAS 79..104
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 151
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 161
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 166
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 176
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 48..117
FT /evidence="ECO:0000250|UniProtKB:Q03431"
FT DISULFID 108..148
FT /evidence="ECO:0000250|UniProtKB:Q03431"
FT DISULFID 131..170
FT /evidence="ECO:0000250|UniProtKB:Q03431"
FT CONFLICT 27..28
FT /note="DA -> TS (in Ref. 2; AAA40011)"
FT /evidence="ECO:0000305"
FT CONFLICT 464..465
FT /note="Missing (in Ref. 2; AAA40011)"
FT /evidence="ECO:0000305"
FT CONFLICT 500..501
FT /note="VS -> GA (in Ref. 1; CAA55536)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 591 AA; 66372 MW; C2E6CCAF2ABAFEEF CRC64;
MGTARIAPSL ALLLCCPVLS SAYALVDADD VFTKEEQIFL LHRAQAQCDK LLKEVLHTAA
NIMESDKGWT PASTSGKPRK EKAPGKFYPE SKENKDVPTG SRRRGRPCLP EWDNIVCWPL
GAPGEVVAVP CPDYIYDFNH KGHAYRRCDR NGSWEVVPGH NRTWANYSEC LKFMTNETRE
REVFDRLGMI YTVGYSMSLA SLTVAVLILA YFRRLHCTRN YIHMHMFLSF MLRAASIFVK
DAVLYSGFTL DEAERLTEEE LHIIAQVPPP PAAAAVGYAG CRVAVTFFLY FLATNYYWIL
VEGLYLHSLI FMAFFSEKKY LWGFTIFGWG LPAVFVAVWV GVRATLANTG CWDLSSGHKK
WIIQVPILAS VVLNFILFIN IIRVLATKLR ETNAGRCDTR QQYRKLLRST LVLVPLFGVH
YTVFMALPYT EVSGTLWQIQ MHYEMLFNSF QGFFVAIIYC FCNGEVQAEI RKSWSRWTLA
LDFKRKARSG SSSYSYGPMV SHTSVTNVGP RAGLSLPLSP RLLPATTNGH SQLPGHAKPG
APAIENETIP VTMTVPKDDG FLNGSCSGLD EEASGSARPP PLLQEEWETV M
