PTHC_CLOB8
ID PTHC_CLOB8 Reviewed; 182 AA.
AC O32332; A6LQ94;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=PTS system glucitol/sorbitol-specific EIIC component {ECO:0000250|UniProtKB:P56579};
DE AltName: Full=EIIC-Gut {ECO:0000250|UniProtKB:P56579};
DE AltName: Full=Glucitol/sorbitol permease IIC component {ECO:0000250|UniProtKB:P56579};
GN Name=srlA; Synonyms=gutA1; OrderedLocusNames=Cbei_0336;
OS Clostridium beijerinckii (strain ATCC 51743 / NCIMB 8052) (Clostridium
OS acetobutylicum).
OC Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=290402;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RX PubMed=9572925; DOI=10.1128/aem.64.5.1612-1619.1998;
RA Tangney M., Brehm J.K., Minton N.P., Mitchell W.J.;
RT "A gene system for glucitol transport and metabolism in Clostridium
RT beijerinckii NCIMB 8052.";
RL Appl. Environ. Microbiol. 64:1612-1619(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51743 / NCIMB 8052;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA Sims D., Brettin T., Bruce D., Tapia R., Brainard J., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Bennet G.,
RA Cann I., Chen J.-S., Contreras A.L., Jones D., Kashket E., Mitchell W.,
RA Stoddard S., Schwarz W., Qureshi N., Young M., Shi Z., Ezeji T., White B.,
RA Blaschek H., Richardson P.;
RT "Complete sequence of Clostridium beijerinckii NCIMB 8052.";
RL Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC system (PTS), a major carbohydrate active transport system, catalyzes
CC the phosphorylation of incoming sugar substrates concomitant with their
CC translocation across the cell membrane. The enzyme II complex composed
CC of SrlA, SrlB and SrlE is involved in glucitol/sorbitol transport.
CC {ECO:0000305|PubMed:9572925}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00430}; Multi-pass membrane protein {ECO:0000255|PROSITE-
CC ProRule:PRU00430}.
CC -!- INDUCTION: Activated by sorbitol and repressed by glucose.
CC {ECO:0000269|PubMed:9572925}.
CC -!- DOMAIN: The EIIC domain forms the PTS system translocation channel and
CC contains the specific substrate-binding site. {ECO:0000255|PROSITE-
CC ProRule:PRU00430}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ002527; CAA05513.1; -; Genomic_DNA.
DR EMBL; CP000721; ABR32524.1; -; Genomic_DNA.
DR RefSeq; WP_011967685.1; NC_009617.1.
DR AlphaFoldDB; O32332; -.
DR STRING; 290402.Cbei_0336; -.
DR TCDB; 4.A.4.1.2; the pts glucitol (gut) family.
DR EnsemblBacteria; ABR32524; ABR32524; Cbei_0336.
DR GeneID; 66343213; -.
DR KEGG; cbe:Cbei_0336; -.
DR eggNOG; COG3730; Bacteria.
DR HOGENOM; CLU_093147_0_0_9; -.
DR OMA; HINPGEY; -.
DR OrthoDB; 1287700at2; -.
DR Proteomes; UP000000565; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR InterPro; IPR004699; PTS_IID_sorb.
DR PANTHER; PTHR40399; PTHR40399; 1.
DR Pfam; PF03608; EII-GUT; 1.
DR PIRSF; PIRSF038321; PTS_glc_srb_IIC; 1.
DR TIGRFAMs; TIGR00821; EII-GUT; 1.
DR PROSITE; PS51107; PTS_EIIC_TYPE_5; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Phosphotransferase system; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..182
FT /note="PTS system glucitol/sorbitol-specific EIIC
FT component"
FT /id="PRO_0000186567"
FT TRANSMEM 28..48
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00430"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00430"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00430"
FT DOMAIN 1..182
FT /note="PTS EIIC type-5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00430"
SQ SEQUENCE 182 AA; 20185 MW; 58EBE4D822677154 CRC64;
MDAIVYFAKG FMYLFEVGGN TFVSWVTGII PKVLLLLVFM NSIIAFIGQD KVDRFAKFAS
RNVILAYGVL PFLSAFMLGN PMALSMGKFL PERMKPSYYA SASYHCHTNS GIFPHINVGE
IFIYLGIANG ITTLGLDPTA LGLRYLLVGL VMNFFAGWVT DFTTKIVMRQ QGIELSNQLK
AN