ID   PTHC_ERWAM              Reviewed;         182 AA.
AC   O32521;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=PTS system glucitol/sorbitol-specific EIIC component {ECO:0000250|UniProtKB:P56579};
DE   AltName: Full=EIIC-Gut {ECO:0000250|UniProtKB:P56579};
DE   AltName: Full=Glucitol/sorbitol permease IIC component {ECO:0000250|UniProtKB:P56579};
GN   Name=srlA;
OS   Erwinia amylovora (Fire blight bacteria).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Erwinia.
OX   NCBI_TaxID=552;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RC   STRAIN=EA7/74;
RX   PubMed=9435786; DOI=10.1007/s004380050609;
RA   Aldridge P., Metzger M., Geider K.;
RT   "Genetics of sorbitol metabolism in Erwinia amylovora and its influence on
RT   bacterial virulence.";
RL   Mol. Gen. Genet. 256:611-619(1997).
CC   -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC       system (PTS), a major carbohydrate active transport system, catalyzes
CC       the phosphorylation of incoming sugar substrates concomitant with their
CC       translocation across the cell membrane. The enzyme II complex composed
CC       of SrlA, SrlB and SrlE is involved in glucitol/sorbitol transport.
CC       {ECO:0000305|PubMed:9435786}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00430}; Multi-pass membrane protein {ECO:0000255|PROSITE-
CC       ProRule:PRU00430}.
CC   -!- INDUCTION: Activated by sorbitol and repressed by glucose.
CC       {ECO:0000269|PubMed:9435786}.
CC   -!- DOMAIN: The EIIC domain forms the PTS system translocation channel and
CC       contains the specific substrate-binding site. {ECO:0000255|PROSITE-
CC       ProRule:PRU00430}.
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DR   EMBL; Y14603; CAA74941.1; -; Genomic_DNA.
DR   RefSeq; WP_004159991.1; NZ_RQKG01000018.1.
DR   AlphaFoldDB; O32521; -.
DR   GeneID; 8914017; -.
DR   OMA; HINPGEY; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR   InterPro; IPR004699; PTS_IID_sorb.
DR   PANTHER; PTHR40399; PTHR40399; 1.
DR   Pfam; PF03608; EII-GUT; 1.
DR   PIRSF; PIRSF038321; PTS_glc_srb_IIC; 1.
DR   TIGRFAMs; TIGR00821; EII-GUT; 1.
DR   PROSITE; PS51107; PTS_EIIC_TYPE_5; 1.
PE   2: Evidence at transcript level;
KW   Cell inner membrane; Cell membrane; Membrane; Phosphotransferase system;
KW   Sugar transport; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..182
FT                   /note="PTS system glucitol/sorbitol-specific EIIC
FT                   component"
FT                   /id="PRO_0000186566"
FT   TRANSMEM        29..49
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00430"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00430"
FT   TRANSMEM        132..152
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00430"
FT   DOMAIN          1..182
FT                   /note="PTS EIIC type-5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00430"
SQ   SEQUENCE   182 AA;  20000 MW;  FC372C4312ABCE39 CRC64;
     MIEAITHGAE WFIGLFQKGG EVFVGMVTGI LPLLISLLVI MNALIVFVGQ RRIEKLAQKC
     AGNPVTRYLV LPFIGTFVFC NPMTHSLGKF LPEKYKPSYY AAASYSCHSM NGLFPHINPG
     ELFVYLGIAN GLTTLGVPLG PLAVSYLLVG LITNFFRGWV TDLTTSVFEK KMGIKLDKSV
     HL