ID   PTHP_CUPNH              Reviewed;          89 AA.
AC   P23537; Q0KEU5;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   25-MAY-2022, entry version 135.
DE   RecName: Full=Phosphocarrier protein HPr;
DE   AltName: Full=Histidine-containing protein;
DE            Short=Protein H;
GN   Name=phbH; Synonyms=ptsH; OrderedLocusNames=H16_A0325;
OS   Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442
OS   / H16 / Stanier 337) (Ralstonia eutropha).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=381666;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1653223; DOI=10.1128/jb.173.18.5843-5853.1991;
RA   Pries A., Priefert H., Krueger N., Steinbuechel A.;
RT   "Identification and characterization of two Alcaligenes eutrophus gene loci
RT   relevant to the poly(beta-hydroxybutyric acid)-leaky phenotype which
RT   exhibit homology to ptsH and ptsI of Escherichia coli.";
RL   J. Bacteriol. 173:5843-5853(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337;
RX   PubMed=16964242; DOI=10.1038/nbt1244;
RA   Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R.,
RA   Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I.,
RA   Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.;
RT   "Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia
RT   eutropha H16.";
RL   Nat. Biotechnol. 24:1257-1262(2006).
CC   -!- FUNCTION: General (non sugar-specific) component of the
CC       phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar
CC       PTS). This major carbohydrate active-transport system catalyzes the
CC       phosphorylation of incoming sugar substrates concomitantly with their
CC       translocation across the cell membrane. The phosphoryl group from
CC       phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier
CC       protein HPr by enzyme I. Phospho-HPr then transfers it to the PTS EIIA
CC       domain. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the HPr family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M69036; AAA21977.1; -; Genomic_DNA.
DR   EMBL; AM260479; CAJ91476.1; -; Genomic_DNA.
DR   PIR; A38120; A38120.
DR   RefSeq; WP_010814838.1; NZ_CP039287.1.
DR   AlphaFoldDB; P23537; -.
DR   SMR; P23537; -.
DR   STRING; 381666.H16_A0325; -.
DR   EnsemblBacteria; CAJ91476; CAJ91476; H16_A0325.
DR   GeneID; 57642422; -.
DR   KEGG; reh:H16_A0325; -.
DR   eggNOG; COG1925; Bacteria.
DR   HOGENOM; CLU_136230_1_1_4; -.
DR   OMA; SIMAMMM; -.
DR   OrthoDB; 404807at2; -.
DR   Proteomes; UP000008210; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR   CDD; cd00367; PTS-HPr_like; 1.
DR   Gene3D; 3.30.1340.10; -; 1.
DR   InterPro; IPR000032; HPr-like.
DR   InterPro; IPR035895; HPr-like_sf.
DR   InterPro; IPR001020; PTS_HPr_His_P_site.
DR   InterPro; IPR002114; PTS_HPr_Ser_P_site.
DR   Pfam; PF00381; PTS-HPr; 1.
DR   PRINTS; PR00107; PHOSPHOCPHPR.
DR   SUPFAM; SSF55594; SSF55594; 1.
DR   TIGRFAMs; TIGR01003; PTS_HPr_family; 1.
DR   PROSITE; PS51350; PTS_HPR_DOM; 1.
DR   PROSITE; PS00369; PTS_HPR_HIS; 1.
DR   PROSITE; PS00589; PTS_HPR_SER; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Phosphotransferase system; Reference proteome; Sugar transport;
KW   Transport.
FT   CHAIN           1..89
FT                   /note="Phosphocarrier protein HPr"
FT                   /id="PRO_0000107837"
FT   DOMAIN          1..88
FT                   /note="HPr"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00681"
FT   ACT_SITE        15
FT                   /note="Pros-phosphohistidine intermediate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00681"
SQ   SEQUENCE   89 AA;  9476 MW;  4275745454116877 CRC64;
     MLQRDTTIIN KLGLHARASA KLTQLAGNFV SQVKMSRNGR QVDAKSIMGV MMLAAGIGST
     VTLETDGPDE QEAMDALLAL IANRFGEGE