ID   PTHP_STRCO              Reviewed;          93 AA.
AC   O50515;
DT   02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-MAY-2022, entry version 126.
DE   RecName: Full=Phosphocarrier protein HPr;
DE   AltName: Full=Histidine-containing protein;
GN   Name=ptsH; OrderedLocusNames=SCO5841; ORFNames=SC9B10.08c;
OS   Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces albidoflavus group.
OX   NCBI_TaxID=100226;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=12000953; DOI=10.1038/417141a;
RA   Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA   Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA   Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA   Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA   Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA   Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA   Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA   Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT   "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT   A3(2).";
RL   Nature 417:141-147(2002).
RN   [2]
RP   PROTEIN SEQUENCE OF 2-19, CHARACTERIZATION, AND MUTAGENESIS OF HIS-15.
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=10491187; DOI=10.1046/j.1432-1327.1999.00727.x;
RA   Parche S., Schmid R., Titgemeyer F.;
RT   "The phosphotransferase system (PTS) of Streptomyces coelicolor
RT   identification and biochemical analysis of a histidine phosphocarrier
RT   protein HPr encoded by the gene ptsH.";
RL   Eur. J. Biochem. 265:308-317(1999).
CC   -!- FUNCTION: General (non sugar-specific) component of the
CC       phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar
CC       PTS). This major carbohydrate active-transport system catalyzes the
CC       phosphorylation of incoming sugar substrates concomitantly with their
CC       translocation across the cell membrane. The phosphoryl group from
CC       phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier
CC       protein HPr by enzyme I. Phospho-HPr then transfers it to the PTS EIIA
CC       domain.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the HPr family. {ECO:0000305}.
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DR   EMBL; AL939125; CAA15798.1; -; Genomic_DNA.
DR   PIR; T35877; T35877.
DR   RefSeq; NP_629964.1; NC_003888.3.
DR   RefSeq; WP_003973180.1; NZ_VNID01000007.1.
DR   AlphaFoldDB; O50515; -.
DR   SMR; O50515; -.
DR   STRING; 100226.SCO5841; -.
DR   TCDB; 8.A.8.1.7; the phosphotransferase system hpr (hpr) family.
DR   GeneID; 1101283; -.
DR   KEGG; sco:SCO5841; -.
DR   PATRIC; fig|100226.15.peg.5938; -.
DR   eggNOG; COG1925; Bacteria.
DR   HOGENOM; CLU_136230_3_2_11; -.
DR   InParanoid; O50515; -.
DR   OMA; RRVNVGW; -.
DR   PhylomeDB; O50515; -.
DR   Proteomes; UP000001973; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IMP:CACAO.
DR   CDD; cd00367; PTS-HPr_like; 1.
DR   Gene3D; 3.30.1340.10; -; 1.
DR   InterPro; IPR000032; HPr-like.
DR   InterPro; IPR035895; HPr-like_sf.
DR   InterPro; IPR001020; PTS_HPr_His_P_site.
DR   Pfam; PF00381; PTS-HPr; 1.
DR   PRINTS; PR00107; PHOSPHOCPHPR.
DR   SUPFAM; SSF55594; SSF55594; 1.
DR   TIGRFAMs; TIGR01003; PTS_HPr_family; 1.
DR   PROSITE; PS51350; PTS_HPR_DOM; 1.
DR   PROSITE; PS00369; PTS_HPR_HIS; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Phosphotransferase system;
KW   Reference proteome; Sugar transport; Transport.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:10491187"
FT   CHAIN           2..93
FT                   /note="Phosphocarrier protein HPr"
FT                   /id="PRO_0000107882"
FT   DOMAIN          2..89
FT                   /note="HPr"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00681"
FT   ACT_SITE        15
FT                   /note="Pros-phosphohistidine intermediate"
FT   MUTAGEN         15
FT                   /note="H->A: Loss of phosphorylation."
FT                   /evidence="ECO:0000269|PubMed:10491187"
SQ   SEQUENCE   93 AA;  9391 MW;  6F72F379FEFFFF19 CRC64;
     MAERRVNVGW AEGLHARPAS IFVRAATATG VPVTIAKADG SPVNAASMLA VLGLGAQGGE
     EIVLASDAEG AEAALERLAK LVAEGLEELP ETV