PTHX1_XANOP
ID PTHX1_XANOP Reviewed; 1373 AA.
AC B2SU53; Q6RKD2;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2013, sequence version 2.
DT 25-MAY-2022, entry version 67.
DE RecName: Full=TAL effector protein PthXo1 {ECO:0000303|PubMed:22223736};
DE AltName: Full=Avirulence protein PthXo1 {ECO:0000303|PubMed:15553245};
DE AltName: Full=TAL effector protein Tal2b {ECO:0000303|PubMed:18452608};
GN Name=pthXo1; Synonyms=tal2b; OrderedLocusNames=PXO_00227;
OS Xanthomonas oryzae pv. oryzae (strain PXO99A).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Xanthomonas.
OX NCBI_TaxID=360094;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PXO99A;
RX PubMed=18452608; DOI=10.1186/1471-2164-9-204;
RA Salzberg S.L., Sommer D.D., Schatz M.C., Phillippy A.M., Rabinowicz P.D.,
RA Tsuge S., Furutani A., Ochiai H., Delcher A.L., Kelley D., Madupu R.,
RA Puiu D., Radune D., Shumway M., Trapnell C., Aparna G., Jha G., Pandey A.,
RA Patil P.B., Ishihara H., Meyer D.F., Szurek B., Verdier V., Koebnik R.,
RA Dow J.M., Ryan R.P., Hirata H., Tsuyumu S., Won Lee S., Seo Y.-S.,
RA Sriariyanum M., Ronald P.C., Sonti R.V., Van Sluys M.-A., Leach J.E.,
RA White F.F., Bogdanove A.J.;
RT "Genome sequence and rapid evolution of the rice pathogen Xanthomonas
RT oryzae pv. oryzae PXO99A.";
RL BMC Genomics 9:204-204(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 276-1241, FUNCTION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=PXO99A;
RX PubMed=15553245; DOI=10.1094/mpmi.2004.17.11.1192;
RA Yang B., White F.F.;
RT "Diverse members of the AvrBs3/PthA family of type III effectors are major
RT virulence determinants in bacterial blight disease of rice.";
RL Mol. Plant Microbe Interact. 17:1192-1200(2004).
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, AND INDUCTION OF HOST GENES.
RC STRAIN=PXO99A;
RX PubMed=16798873; DOI=10.1073/pnas.0604088103;
RA Yang B., Sugio A., White F.F.;
RT "Os8N3 is a host disease-susceptibility gene for bacterial blight of
RT rice.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:10503-10508(2006).
RN [4]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=20345643; DOI=10.1111/j.1469-8137.2010.03217.x;
RA Roemer P., Recht S., Strauss T., Elsaesser J., Schornack S., Boch J.,
RA Wang S., Lahaye T.;
RT "Promoter elements of rice susceptibility genes are bound and activated by
RT specific TAL effectors from the bacterial blight pathogen, Xanthomonas
RT oryzae pv. oryzae.";
RL New Phytol. 187:1048-1057(2010).
RN [5]
RP BIOTECHNOLOGY.
RX PubMed=20660643; DOI=10.1534/genetics.110.120717;
RA Christian M., Cermak T., Doyle E.L., Schmidt C., Zhang F., Hummel A.,
RA Bogdanove A.J., Voytas D.F.;
RT "Targeting DNA double-strand breaks with TAL effector nucleases.";
RL Genetics 186:757-761(2010).
RN [6]
RP BIOTECHNOLOGY USES REVIEW.
RX PubMed=25057889; DOI=10.1042/bj20140295;
RA Wright D.A., Li T., Yang B., Spalding M.H.;
RT "TALEN-mediated genome editing: prospects and perspectives.";
RL Biochem. J. 462:15-24(2014).
RN [7]
RP BIOTECHNOLOGY USES REVIEW.
RX PubMed=24602153; DOI=10.1111/tpj.12431;
RA de Lange O., Binder A., Lahaye T.;
RT "From dead leaf, to new life: TAL effectors as tools for synthetic
RT biology.";
RL Plant J. 78:753-771(2014).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) OF 127-1149 IN COMPLEX WITH DNA,
RP DNA-BINDING, AND DOMAIN.
RC STRAIN=PXO99A;
RX PubMed=22223736; DOI=10.1126/science.1216211;
RA Mak A.N., Bradley P., Cernadas R.A., Bogdanove A.J., Stoddard B.L.;
RT "The crystal structure of TAL effector PthXo1 bound to its DNA target.";
RL Science 335:716-719(2012).
CC -!- FUNCTION: Avirulence protein. Acts as a transcription factor in rice,
CC inducing expression of a number of host genes including SWEET11 (Os8N3,
CC XA13, AC Q6YZF3) in susceptible plants with the Xa13 allele. Plants
CC with the xa13 allele, which has an altered promoter, are resistant to
CC bacterial blight caused by this bacterial strain and do not induce
CC SWEET11. The xa13 allele elicits an atypical hypersensitive response
CC (HR). PthXo1 binds SWEET11 promoter DNA in a sequence-specific manner.
CC {ECO:0000269|PubMed:15553245, ECO:0000269|PubMed:16798873,
CC ECO:0000269|PubMed:20345643, ECO:0000269|PubMed:22223736}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P14727}. Host
CC nucleus {ECO:0000250|UniProtKB:P14727}. Note=Secreted via a type III
CC secretions system (TTSS). Localizes to the plant cell nucleus.
CC {ECO:0000250|UniProtKB:P14727}.
CC -!- DOMAIN: The central DNA-binding region is composed of 23.5 tandem core
CC repeats with 1 base-specifying residue (BSR residue 13, also called the
CC repeat variable diresidue, RVD, residues 12 and 13) each of which
CC recognizes 1 base in the target DNA. The BSR is the only residue which
CC contacts DNA in a sequence-specific manner.
CC {ECO:0000269|PubMed:22223736}.
CC -!- DISRUPTION PHENOTYPE: Reduced virulence in susceptible rice; its
CC introduction into a deletion mutant restores virulence to the bacteria
CC in rice plants. No longer induces SWEET11 (Os8N3, XA13, AC Q6YZF3).
CC {ECO:0000269|PubMed:15553245, ECO:0000269|PubMed:16798873}.
CC -!- BIOTECHNOLOGY: By combining the central DNA-binding domain with the
CC catalytic domain of the restriction endonuclease FokI, TALE-nuclease
CC (TALEN) enzymes able to target specific dsDNA sequences can be created
CC that enable eukaryotic genome modification. Other potential uses as
CC transcriptional repressors, for transposon targeting, DNA methylation
CC or histone tail modifictions are also possible.
CC {ECO:0000269|PubMed:20660643, ECO:0000303|PubMed:24602153,
CC ECO:0000303|PubMed:25057889}.
CC -!- SIMILARITY: Belongs to the transcription activator-like effector (TALE)
CC family. {ECO:0000305}.
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DR EMBL; CP000967; ACD58243.2; -; Genomic_DNA.
DR EMBL; AY495676; AAS46025.1; -; Genomic_DNA.
DR RefSeq; WP_041182630.1; NC_010717.2.
DR PDB; 3UGM; X-ray; 3.00 A; A=127-1149.
DR PDBsum; 3UGM; -.
DR AlphaFoldDB; B2SU53; -.
DR SMR; B2SU53; -.
DR STRING; 360094.PXO_00227; -.
DR EnsemblBacteria; ACD58243; ACD58243; PXO_00227.
DR KEGG; xop:PXO_00227; -.
DR eggNOG; COG2201; Bacteria.
DR HOGENOM; CLU_003229_1_0_6; -.
DR OrthoDB; 41142at2; -.
DR PHI-base; PHI:2718; -.
DR PHI-base; PHI:6182; -.
DR PHI-base; PHI:8171; -.
DR PHI-base; PHI:8686; -.
DR Proteomes; UP000001740; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0034053; P:modulation by symbiont of host defense-related programmed cell death; IEA:UniProtKB-KW.
DR InterPro; IPR005042; TAL_effector_rpt.
DR Pfam; PF03377; TAL_effector; 23.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Host nucleus;
KW Hypersensitive response elicitation; Repeat; Secreted; Transcription;
KW Transcription regulation; Virulence.
FT CHAIN 1..1373
FT /note="TAL effector protein PthXo1"
FT /id="PRO_0000430621"
FT REPEAT 221..239
FT /note="Cryptic repeat -1"
FT /evidence="ECO:0000303|PubMed:22223736"
FT REPEAT 256..273
FT /note="Cryptic repeat 0"
FT /evidence="ECO:0000303|PubMed:22223736"
FT REPEAT 289..322
FT /note="Core repeat 1"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 323..356
FT /note="Core repeat 2"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 357..390
FT /note="Core repeat 3"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 391..424
FT /note="Core repeat 4"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 425..458
FT /note="Core repeat 5"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 459..492
FT /note="Core repeat 6"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 493..525
FT /note="Core repeat 7"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 526..559
FT /note="Core repeat 8"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 560..593
FT /note="Core repeat 9"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 594..627
FT /note="Core repeat 10"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 628..661
FT /note="Core repeat 11"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 662..695
FT /note="Core repeat 12"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 696..729
FT /note="Core repeat 13"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 714..760
FT /note="HEAT 1"
FT /evidence="ECO:0000255"
FT REPEAT 730..763
FT /note="Core repeat 14"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 764..797
FT /note="Core repeat 15"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 782..828
FT /note="HEAT 2"
FT /evidence="ECO:0000255"
FT REPEAT 798..831
FT /note="Core repeat 16"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 832..865
FT /note="Core repeat 17"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 850..893
FT /note="HEAT 3"
FT /evidence="ECO:0000255"
FT REPEAT 866..899
FT /note="Core repeat 18"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 900..933
FT /note="Core repeat 19"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 918..961
FT /note="HEAT 4"
FT /evidence="ECO:0000255"
FT REPEAT 934..967
FT /note="Core repeat 20"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 968..1001
FT /note="Core repeat 21"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 1002..1034
FT /note="Core repeat 22"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 1035..1068
FT /note="Core repeat 23"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REPEAT 1053..1091
FT /note="HEAT 5"
FT /evidence="ECO:0000255"
FT REPEAT 1069..1087
FT /note="Core repeat 23.5"
FT /evidence="ECO:0000303|PubMed:15553245"
FT REGION 1..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 127..152
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1136..1364
FT /note="Acidic activation domain"
FT /evidence="ECO:0000250|UniProtKB:P14727"
FT REGION 1250..1286
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 1222..1225
FT /note="Nuclear localization signal NLS1"
FT /evidence="ECO:0000250|UniProtKB:P14727"
FT MOTIF 1268..1271
FT /note="Nuclear localization signal NLS2"
FT /evidence="ECO:0000250|UniProtKB:P14727"
FT MOTIF 1305..1308
FT /note="Nuclear localization signal NLS3"
FT /evidence="ECO:0000250|UniProtKB:P14727"
FT CONFLICT 358
FT /note="P -> T (in Ref. 2; AAS46025)"
FT /evidence="ECO:0000305"
FT CONFLICT 1120
FT /note="M -> L (in Ref. 2; AAS46025)"
FT /evidence="ECO:0000305"
FT CONFLICT 1137
FT /note="V -> I (in Ref. 2; AAS46025)"
FT /evidence="ECO:0000305"
FT CONFLICT 1142
FT /note="G -> P (in Ref. 2; AAS46025)"
FT /evidence="ECO:0000305"
FT CONFLICT 1150..1154
FT /note="ADYAQ -> PDLAH (in Ref. 2; AAS46025)"
FT /evidence="ECO:0000305"
FT CONFLICT 1160..1164
FT /note="EFFQC -> GFFQS (in Ref. 2; AAS46025)"
FT /evidence="ECO:0000305"
FT CONFLICT 1170..1174
FT /note="YAFDE -> QAFDD (in Ref. 2; AAS46025)"
FT /evidence="ECO:0000305"
FT CONFLICT 1184
FT /note="N -> H (in Ref. 2; AAS46025)"
FT /evidence="ECO:0000305"
FT CONFLICT 1198..1202
FT /note="LEARG -> FEARY (in Ref. 2; AAS46025)"
FT /evidence="ECO:0000305"
FT HELIX 222..228
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 234..245
FT /evidence="ECO:0007829|PDB:3UGM"
FT TURN 246..252
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 258..266
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 268..282
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 291..298
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 303..313
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 315..319
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 325..328
FT /evidence="ECO:0007829|PDB:3UGM"
FT TURN 329..333
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 337..354
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 359..366
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 371..381
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 383..389
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 393..400
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 405..422
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 427..434
FT /evidence="ECO:0007829|PDB:3UGM"
FT STRAND 435..438
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 440..454
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 461..468
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 473..488
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 495..504
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 506..521
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 528..535
FT /evidence="ECO:0007829|PDB:3UGM"
FT TURN 538..540
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 541..555
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 562..568
FT /evidence="ECO:0007829|PDB:3UGM"
FT STRAND 569..571
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 574..591
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 597..603
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 608..618
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 620..622
FT /evidence="ECO:0007829|PDB:3UGM"
FT TURN 623..626
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 631..637
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 643..656
FT /evidence="ECO:0007829|PDB:3UGM"
FT TURN 657..659
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 664..670
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 676..686
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 688..694
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 699..702
FT /evidence="ECO:0007829|PDB:3UGM"
FT TURN 703..706
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 711..727
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 733..739
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 744..754
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 757..760
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 766..769
FT /evidence="ECO:0007829|PDB:3UGM"
FT TURN 770..773
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 778..788
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 800..807
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 812..826
FT /evidence="ECO:0007829|PDB:3UGM"
FT STRAND 829..831
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 835..841
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 846..854
FT /evidence="ECO:0007829|PDB:3UGM"
FT TURN 855..859
FT /evidence="ECO:0007829|PDB:3UGM"
FT TURN 861..863
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 868..875
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 880..897
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 902..909
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 914..929
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 936..943
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 948..958
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 960..964
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 970..976
FT /evidence="ECO:0007829|PDB:3UGM"
FT STRAND 979..981
FT /evidence="ECO:0007829|PDB:3UGM"
FT TURN 982..984
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 985..997
FT /evidence="ECO:0007829|PDB:3UGM"
FT TURN 998..1000
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 1004..1011
FT /evidence="ECO:0007829|PDB:3UGM"
FT TURN 1012..1014
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 1015..1023
FT /evidence="ECO:0007829|PDB:3UGM"
FT HELIX 1026..1031
FT /evidence="ECO:0007829|PDB:3UGM"
FT TURN 1037..1039
FT /evidence="ECO:0007829|PDB:3UGM"
FT STRAND 1040..1042
FT /evidence="ECO:0007829|PDB:3UGM"
SQ SEQUENCE 1373 AA; 143535 MW; C96C8A8A862A8217 CRC64;
MDPIRSRTPS PARELLPGPQ PDRVQPTADR GGAPPAGGPL DGLPARRTMS RTRLPSPPAP
SPAFSAGSFS DLLRQFDPSL LDTSLLDSMP AVGTPHTAAA PAECDEVQSG LRAADDPPPT
VRVAVTAARP PRAKPAPRRR AAQPSDASPA AQVDLRTLGY SQQQQEKIKP KVGSTVAQHH
EALVGHGFTH AHIVALSRHP AALGTVAVKY QDMIAALPEA THEDIVGVGK QWSGARALEA
LLTVAGELRG PPLQLDTGQL VKIAKRGGVT AVEAVHASRN ALTGAPLNLT PAQVVAIASN
NGGKQALETV QRLLPVLCQA HGLTPAQVVA IASHDGGKQA LETMQRLLPV LCQAHGLPPD
QVVAIASNIG GKQALETVQR LLPVLCQAHG LTPDQVVAIA SHGGGKQALE TVQRLLPVLC
QAHGLTPDQV VAIASHDGGK QALETVQRLL PVLCQAHGLT PDQVVAIASN GGGKQALETV
QRLLPVLCQA HGLTPDQVVA IASNGGKQAL ETVQRLLPVL CQAHGLTPDQ VVAIASHDGG
KQALETVQRL LPVLCQTHGL TPAQVVAIAS HDGGKQALET VQQLLPVLCQ AHGLTPDQVV
AIASNIGGKQ ALATVQRLLP VLCQAHGLTP DQVVAIASNG GGKQALETVQ RLLPVLCQAH
GLTPDQVVAI ASNGGGKQAL ETVQRLLPVL CQAHGLTQVQ VVAIASNIGG KQALETVQRL
LPVLCQAHGL TPAQVVAIAS HDGGKQALET VQRLLPVLCQ AHGLTPDQVV AIASNGGGKQ
ALETVQRLLP VLCQAHGLTQ EQVVAIASNN GGKQALETVQ RLLPVLCQAH GLTPDQVVAI
ASNGGGKQAL ETVQRLLPVL CQAHGLTPAQ VVAIASNIGG KQALETVQRL LPVLCQDHGL
TLAQVVAIAS NIGGKQALET VQRLLPVLCQ AHGLTQDQVV AIASNIGGKQ ALETVQRLLP
VLCQDHGLTP DQVVAIASNI GGKQALETVQ RLLPVLCQDH GLTLDQVVAI ASNGGKQALE
TVQRLLPVLC QDHGLTPDQV VAIASNSGGK QALETVQRLL PVLCQDHGLT PNQVVAIASN
GGKQALESIV AQLSRPDPAL AALTNDHLVA LACLGGRPAM DAVKKGLPHA PELIRRVNRR
IGERTSHRVA DYAQVVRVLE FFQCHSHPAY AFDEAMTQFG MSRNGLVQLF RRVGVTELEA
RGGTLPPASQ RWDRILQASG MKRAKPSPTS AQTPDQASLH AFADSLERDL DAPSPMHEGD
QTGASSRKRS RSDRAVTGPS AQHSFEVRVP EQRDALHLPL SWRVKRPRTR IGGGLPDPGT
PIAADLAASS TVMWEQDAAP FAGAADDFPA FNEEELAWLM ELLPQSGSVG GTI
