ID   PTH_KLEP7               Reviewed;         194 AA.
AC   A6TAP7;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Peptidyl-tRNA hydrolase {ECO:0000255|HAMAP-Rule:MF_00083};
DE            Short=PTH {ECO:0000255|HAMAP-Rule:MF_00083};
DE            EC=3.1.1.29 {ECO:0000255|HAMAP-Rule:MF_00083};
GN   Name=pth {ECO:0000255|HAMAP-Rule:MF_00083};
GN   OrderedLocusNames=KPN78578_22070; ORFNames=KPN_02242;
OS   Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=272620;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700721 / MGH 78578;
RG   The Klebsiella pneumonia Genome Sequencing Project;
RA   McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P.,
RA   Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K.;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The natural substrate for this enzyme may be peptidyl-tRNAs
CC       which drop off the ribosome during protein synthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00083}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-
CC         amino acid + H(+); Xref=Rhea:RHEA:54448, Rhea:RHEA-COMP:10123,
CC         Rhea:RHEA-COMP:13883, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:59874, ChEBI:CHEBI:78442, ChEBI:CHEBI:138191;
CC         EC=3.1.1.29; Evidence={ECO:0000255|HAMAP-Rule:MF_00083};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00083}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00083}.
CC   -!- SIMILARITY: Belongs to the PTH family. {ECO:0000255|HAMAP-
CC       Rule:MF_00083}.
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DR   EMBL; CP000647; ABR77668.1; -; Genomic_DNA.
DR   RefSeq; WP_004180389.1; NC_009648.1.
DR   PDB; 7BRD; X-ray; 1.89 A; A/B=1-194.
DR   PDBsum; 7BRD; -.
DR   AlphaFoldDB; A6TAP7; -.
DR   SMR; A6TAP7; -.
DR   STRING; 272620.KPN_02242; -.
DR   EnsemblBacteria; ABR77668; ABR77668; KPN_02242.
DR   GeneID; 64294783; -.
DR   KEGG; kpn:KPN_02242; -.
DR   HOGENOM; CLU_062456_3_1_6; -.
DR   OMA; HVLSKFH; -.
DR   Proteomes; UP000000265; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004045; F:aminoacyl-tRNA hydrolase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR   CDD; cd00462; PTH; 1.
DR   Gene3D; 3.40.50.1470; -; 1.
DR   HAMAP; MF_00083; Pept_tRNA_hydro_bact; 1.
DR   InterPro; IPR001328; Pept_tRNA_hydro.
DR   InterPro; IPR018171; Pept_tRNA_hydro_CS.
DR   InterPro; IPR036416; Pept_tRNA_hydro_sf.
DR   PANTHER; PTHR17224; PTHR17224; 1.
DR   Pfam; PF01195; Pept_tRNA_hydro; 1.
DR   SUPFAM; SSF53178; SSF53178; 1.
DR   TIGRFAMs; TIGR00447; pth; 1.
DR   PROSITE; PS01195; PEPT_TRNA_HYDROL_1; 1.
DR   PROSITE; PS01196; PEPT_TRNA_HYDROL_2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Hydrolase; Reference proteome.
FT   CHAIN           1..194
FT                   /note="Peptidyl-tRNA hydrolase"
FT                   /id="PRO_1000010597"
FT   STRAND          5..8
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   HELIX           14..17
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   HELIX           20..22
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   HELIX           23..34
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   STRAND          40..42
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   HELIX           43..45
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   STRAND          47..54
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   STRAND          57..64
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   HELIX           68..70
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   HELIX           71..81
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   HELIX           86..88
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   STRAND          89..95
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   STRAND          103..108
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   HELIX           115..123
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   STRAND          130..136
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   HELIX           143..145
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   HELIX           146..150
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   HELIX           156..179
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   HELIX           181..188
FT                   /evidence="ECO:0007829|PDB:7BRD"
FT   TURN            189..193
FT                   /evidence="ECO:0007829|PDB:7BRD"
SQ   SEQUENCE   194 AA;  21100 MW;  50F03717BA9B8BE3 CRC64;
     MTIKLIVGLA NPGAEYAATR HNAGAWYVDL LADRHRAPLR EESKFFGYTS RINLAGEDVR
     LLVPTTFMNL SGKAVAAMAT FYRINPDEIL VAHDELDLPP GVAKFKLGGG HGGHNGLKDI
     ISKLGNNPNF HRLRVGIGHP GDKNKVVGFV LGKPPASEQK LIDDAVDEAA RCTEIWLKDG
     LTKATNRLHA FKAQ