PTH_SACS2
ID PTH_SACS2 Reviewed; 120 AA.
AC Q980V1;
DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2001, sequence version 1.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Peptidyl-tRNA hydrolase {ECO:0000255|HAMAP-Rule:MF_00628};
DE Short=PTH {ECO:0000255|HAMAP-Rule:MF_00628};
DE EC=3.1.1.29 {ECO:0000255|HAMAP-Rule:MF_00628};
GN Name=pth {ECO:0000255|HAMAP-Rule:MF_00628}; OrderedLocusNames=SSO0175;
OS Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS (Sulfolobus solfataricus).
OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC Saccharolobus.
OX NCBI_TaxID=273057;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=11427726; DOI=10.1073/pnas.141222098;
RA She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
RN [2]
RP PROTEIN SEQUENCE OF 1-10, FUNCTION, AND CHARACTERIZATION.
RC STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX PubMed=12799450; DOI=10.1093/nar/gkg428;
RA Fromant M., Ferri-Fioni M.-L., Plateau P., Blanquet S.;
RT "Peptidyl-tRNA hydrolase from Sulfolobus solfataricus.";
RL Nucleic Acids Res. 31:3227-3235(2003).
CC -!- FUNCTION: The natural substrate for this enzyme may be peptidyl-tRNAs
CC which drop off the ribosome during protein synthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00628, ECO:0000269|PubMed:12799450}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-
CC amino acid + H(+); Xref=Rhea:RHEA:54448, Rhea:RHEA-COMP:10123,
CC Rhea:RHEA-COMP:13883, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:59874, ChEBI:CHEBI:78442, ChEBI:CHEBI:138191;
CC EC=3.1.1.29; Evidence={ECO:0000255|HAMAP-Rule:MF_00628};
CC -!- SUBUNIT: Homodimer.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the PTH2 family. {ECO:0000255|HAMAP-
CC Rule:MF_00628}.
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DR EMBL; AE006641; AAK40521.1; -; Genomic_DNA.
DR PIR; B90158; B90158.
DR RefSeq; WP_009990400.1; NC_002754.1.
DR PDB; 1XTY; X-ray; 1.80 A; A/B/C/D=1-120.
DR PDBsum; 1XTY; -.
DR AlphaFoldDB; Q980V1; -.
DR SMR; Q980V1; -.
DR STRING; 273057.SSO0175; -.
DR EnsemblBacteria; AAK40521; AAK40521; SSO0175.
DR GeneID; 44129141; -.
DR KEGG; sso:SSO0175; -.
DR PATRIC; fig|273057.12.peg.171; -.
DR eggNOG; arCOG04228; Archaea.
DR HOGENOM; CLU_073661_2_2_2; -.
DR InParanoid; Q980V1; -.
DR OMA; GHAAVEC; -.
DR PhylomeDB; Q980V1; -.
DR BRENDA; 3.1.1.29; 6163.
DR EvolutionaryTrace; Q980V1; -.
DR Proteomes; UP000001974; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0004045; F:aminoacyl-tRNA hydrolase activity; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR CDD; cd02430; PTH2; 1.
DR Gene3D; 3.40.1490.10; -; 1.
DR HAMAP; MF_00628; Pept_tRNA_hydro_arch; 1.
DR InterPro; IPR023476; Pep_tRNA_hydro_II_dom_sf.
DR InterPro; IPR034759; Pept_tRNA_hydro_arch.
DR InterPro; IPR002833; PTH2.
DR PANTHER; PTHR12649; PTHR12649; 1.
DR Pfam; PF01981; PTH2; 1.
DR SUPFAM; SSF102462; SSF102462; 1.
DR TIGRFAMs; TIGR00283; arch_pth2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Direct protein sequencing; Hydrolase;
KW Reference proteome.
FT CHAIN 1..120
FT /note="Peptidyl-tRNA hydrolase"
FT /id="PRO_0000120304"
FT STRAND 2..12
FT /evidence="ECO:0007829|PDB:1XTY"
FT HELIX 16..35
FT /evidence="ECO:0007829|PDB:1XTY"
FT HELIX 40..51
FT /evidence="ECO:0007829|PDB:1XTY"
FT STRAND 56..63
FT /evidence="ECO:0007829|PDB:1XTY"
FT HELIX 64..76
FT /evidence="ECO:0007829|PDB:1XTY"
FT STRAND 81..85
FT /evidence="ECO:0007829|PDB:1XTY"
FT STRAND 88..92
FT /evidence="ECO:0007829|PDB:1XTY"
FT STRAND 97..106
FT /evidence="ECO:0007829|PDB:1XTY"
FT HELIX 107..114
FT /evidence="ECO:0007829|PDB:1XTY"
SQ SEQUENCE 120 AA; 13140 MW; A90F6A3ADC714B91 CRC64;
MIKMVIVVRS DIKMGKGKIA AQVAHAAVTL VVSIINSNNL RWKEWLNEWL HQGQPKIIVK
VNSLDEIISR AKKAETMNLP FSIIEDAGKT QLEPGTITCL GIGPAPENLV DSITGDLKLL
