PTH_THET8
ID PTH_THET8 Reviewed; 183 AA.
AC Q5SHZ2;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Peptidyl-tRNA hydrolase {ECO:0000255|HAMAP-Rule:MF_00083};
DE Short=PTH {ECO:0000255|HAMAP-Rule:MF_00083};
DE EC=3.1.1.29 {ECO:0000255|HAMAP-Rule:MF_00083};
GN Name=pth {ECO:0000255|HAMAP-Rule:MF_00083}; OrderedLocusNames=TTHA1588;
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The natural substrate for this enzyme may be peptidyl-tRNAs
CC which drop off the ribosome during protein synthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00083}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-
CC amino acid + H(+); Xref=Rhea:RHEA:54448, Rhea:RHEA-COMP:10123,
CC Rhea:RHEA-COMP:13883, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:59874, ChEBI:CHEBI:78442, ChEBI:CHEBI:138191;
CC EC=3.1.1.29; Evidence={ECO:0000255|HAMAP-Rule:MF_00083};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00083}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00083}.
CC -!- SIMILARITY: Belongs to the PTH family. {ECO:0000255|HAMAP-
CC Rule:MF_00083}.
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DR EMBL; AP008226; BAD71411.1; -; Genomic_DNA.
DR RefSeq; WP_011173630.1; NC_006461.1.
DR RefSeq; YP_144854.1; NC_006461.1.
DR PDB; 5ZX8; X-ray; 1.00 A; A=1-183.
DR PDBsum; 5ZX8; -.
DR AlphaFoldDB; Q5SHZ2; -.
DR SMR; Q5SHZ2; -.
DR STRING; 300852.55772970; -.
DR EnsemblBacteria; BAD71411; BAD71411; BAD71411.
DR GeneID; 3169381; -.
DR KEGG; ttj:TTHA1588; -.
DR PATRIC; fig|300852.9.peg.1558; -.
DR eggNOG; COG0193; Bacteria.
DR HOGENOM; CLU_062456_3_1_0; -.
DR OMA; HVLSKFH; -.
DR PhylomeDB; Q5SHZ2; -.
DR BRENDA; 2.3.2.12; 2305.
DR Proteomes; UP000000532; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004045; F:aminoacyl-tRNA hydrolase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR CDD; cd00462; PTH; 1.
DR Gene3D; 3.40.50.1470; -; 1.
DR HAMAP; MF_00083; Pept_tRNA_hydro_bact; 1.
DR InterPro; IPR001328; Pept_tRNA_hydro.
DR InterPro; IPR018171; Pept_tRNA_hydro_CS.
DR InterPro; IPR036416; Pept_tRNA_hydro_sf.
DR PANTHER; PTHR17224; PTHR17224; 1.
DR Pfam; PF01195; Pept_tRNA_hydro; 1.
DR SUPFAM; SSF53178; SSF53178; 1.
DR TIGRFAMs; TIGR00447; pth; 1.
DR PROSITE; PS01195; PEPT_TRNA_HYDROL_1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Hydrolase; Reference proteome.
FT CHAIN 1..183
FT /note="Peptidyl-tRNA hydrolase"
FT /id="PRO_0000187843"
FT STRAND 3..6
FT /evidence="ECO:0007829|PDB:5ZX8"
FT HELIX 12..14
FT /evidence="ECO:0007829|PDB:5ZX8"
FT HELIX 18..20
FT /evidence="ECO:0007829|PDB:5ZX8"
FT HELIX 21..26
FT /evidence="ECO:0007829|PDB:5ZX8"
FT STRAND 34..36
FT /evidence="ECO:0007829|PDB:5ZX8"
FT STRAND 39..44
FT /evidence="ECO:0007829|PDB:5ZX8"
FT STRAND 47..52
FT /evidence="ECO:0007829|PDB:5ZX8"
FT HELIX 56..59
FT /evidence="ECO:0007829|PDB:5ZX8"
FT HELIX 60..70
FT /evidence="ECO:0007829|PDB:5ZX8"
FT HELIX 74..76
FT /evidence="ECO:0007829|PDB:5ZX8"
FT STRAND 77..83
FT /evidence="ECO:0007829|PDB:5ZX8"
FT STRAND 91..96
FT /evidence="ECO:0007829|PDB:5ZX8"
FT HELIX 103..112
FT /evidence="ECO:0007829|PDB:5ZX8"
FT STRAND 117..123
FT /evidence="ECO:0007829|PDB:5ZX8"
FT HELIX 130..132
FT /evidence="ECO:0007829|PDB:5ZX8"
FT HELIX 133..137
FT /evidence="ECO:0007829|PDB:5ZX8"
FT HELIX 143..145
FT /evidence="ECO:0007829|PDB:5ZX8"
FT HELIX 146..166
FT /evidence="ECO:0007829|PDB:5ZX8"
FT HELIX 168..175
FT /evidence="ECO:0007829|PDB:5ZX8"
SQ SEQUENCE 183 AA; 20179 MW; 5D00C36437E8CF1C CRC64;
MFLVVGQGNP GERYARTRHN LGFMVLDRLG LSFRPRGEAL VAEAEGGLFL KPLTYYNLTG
RAVAPLARFY KIPPERILVV HDEMDLPLGR IRFKAGGSAA GNRGVLSIEE ALGTRAFHRL
RLGIGKPPDP SRGAEYVLSP FREEELPVVE RVLEAAKEAV WCWVREGLPP CAGRFNGLDL
SLG
