PTH_VIBCH
ID PTH_VIBCH Reviewed; 196 AA.
AC Q9KQ21;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Peptidyl-tRNA hydrolase {ECO:0000255|HAMAP-Rule:MF_00083};
DE Short=PTH {ECO:0000255|HAMAP-Rule:MF_00083};
DE EC=3.1.1.29 {ECO:0000255|HAMAP-Rule:MF_00083};
GN Name=pth {ECO:0000255|HAMAP-Rule:MF_00083}; OrderedLocusNames=VC_2184;
OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=243277;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
CC -!- FUNCTION: The natural substrate for this enzyme may be peptidyl-tRNAs
CC which drop off the ribosome during protein synthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00083}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an N-acyl-L-alpha-aminoacyl-tRNA + H2O = a tRNA + an N-acyl-L-
CC amino acid + H(+); Xref=Rhea:RHEA:54448, Rhea:RHEA-COMP:10123,
CC Rhea:RHEA-COMP:13883, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:59874, ChEBI:CHEBI:78442, ChEBI:CHEBI:138191;
CC EC=3.1.1.29; Evidence={ECO:0000255|HAMAP-Rule:MF_00083};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00083}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00083}.
CC -!- SIMILARITY: Belongs to the PTH family. {ECO:0000255|HAMAP-
CC Rule:MF_00083}.
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DR EMBL; AE003852; AAF95329.1; -; Genomic_DNA.
DR PIR; C82107; C82107.
DR RefSeq; NP_231815.1; NC_002505.1.
DR RefSeq; WP_000081944.1; NZ_LT906614.1.
DR PDB; 4Z86; X-ray; 1.63 A; A/B=2-196.
DR PDB; 4ZXP; X-ray; 1.63 A; A/B=2-196.
DR PDB; 5B6J; X-ray; 2.43 A; A/B=2-196.
DR PDB; 5EKT; X-ray; 1.63 A; A/B=2-196.
DR PDB; 5IKE; X-ray; 2.09 A; A/B=2-196.
DR PDB; 5IMB; X-ray; 2.40 A; A/B=2-196.
DR PDB; 5IVP; X-ray; 2.01 A; A/B=2-196.
DR PDB; 5ZK0; X-ray; 2.55 A; A/B=2-196.
DR PDBsum; 4Z86; -.
DR PDBsum; 4ZXP; -.
DR PDBsum; 5B6J; -.
DR PDBsum; 5EKT; -.
DR PDBsum; 5IKE; -.
DR PDBsum; 5IMB; -.
DR PDBsum; 5IVP; -.
DR PDBsum; 5ZK0; -.
DR AlphaFoldDB; Q9KQ21; -.
DR BMRB; Q9KQ21; -.
DR SMR; Q9KQ21; -.
DR STRING; 243277.VC_2184; -.
DR DNASU; 2613320; -.
DR EnsemblBacteria; AAF95329; AAF95329; VC_2184.
DR KEGG; vch:VC_2184; -.
DR PATRIC; fig|243277.26.peg.2082; -.
DR eggNOG; COG0193; Bacteria.
DR HOGENOM; CLU_062456_3_1_6; -.
DR OMA; HVLSKFH; -.
DR BioCyc; VCHO:VC2184-MON; -.
DR BRENDA; 3.1.1.29; 15862.
DR Proteomes; UP000000584; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004045; F:aminoacyl-tRNA hydrolase activity; IBA:GO_Central.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-UniRule.
DR CDD; cd00462; PTH; 1.
DR Gene3D; 3.40.50.1470; -; 1.
DR HAMAP; MF_00083; Pept_tRNA_hydro_bact; 1.
DR InterPro; IPR001328; Pept_tRNA_hydro.
DR InterPro; IPR018171; Pept_tRNA_hydro_CS.
DR InterPro; IPR036416; Pept_tRNA_hydro_sf.
DR PANTHER; PTHR17224; PTHR17224; 1.
DR Pfam; PF01195; Pept_tRNA_hydro; 1.
DR SUPFAM; SSF53178; SSF53178; 1.
DR TIGRFAMs; TIGR00447; pth; 1.
DR PROSITE; PS01195; PEPT_TRNA_HYDROL_1; 1.
DR PROSITE; PS01196; PEPT_TRNA_HYDROL_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Hydrolase; Reference proteome.
FT CHAIN 1..196
FT /note="Peptidyl-tRNA hydrolase"
FT /id="PRO_0000187850"
FT STRAND 6..10
FT /evidence="ECO:0007829|PDB:4Z86"
FT TURN 16..20
FT /evidence="ECO:0007829|PDB:4Z86"
FT HELIX 22..24
FT /evidence="ECO:0007829|PDB:4Z86"
FT HELIX 25..36
FT /evidence="ECO:0007829|PDB:4Z86"
FT STRAND 42..44
FT /evidence="ECO:0007829|PDB:4Z86"
FT HELIX 45..47
FT /evidence="ECO:0007829|PDB:4Z86"
FT STRAND 49..56
FT /evidence="ECO:0007829|PDB:4Z86"
FT STRAND 59..66
FT /evidence="ECO:0007829|PDB:4Z86"
FT HELIX 70..72
FT /evidence="ECO:0007829|PDB:4Z86"
FT HELIX 73..83
FT /evidence="ECO:0007829|PDB:4Z86"
FT HELIX 88..90
FT /evidence="ECO:0007829|PDB:4Z86"
FT STRAND 91..97
FT /evidence="ECO:0007829|PDB:4Z86"
FT STRAND 105..109
FT /evidence="ECO:0007829|PDB:4Z86"
FT HELIX 117..125
FT /evidence="ECO:0007829|PDB:4Z86"
FT TURN 126..128
FT /evidence="ECO:0007829|PDB:4Z86"
FT STRAND 132..138
FT /evidence="ECO:0007829|PDB:4Z86"
FT HELIX 145..147
FT /evidence="ECO:0007829|PDB:4Z86"
FT HELIX 148..152
FT /evidence="ECO:0007829|PDB:4Z86"
FT HELIX 158..181
FT /evidence="ECO:0007829|PDB:4Z86"
FT HELIX 183..190
FT /evidence="ECO:0007829|PDB:4Z86"
FT TURN 193..195
FT /evidence="ECO:0007829|PDB:5ZK0"
SQ SEQUENCE 196 AA; 21483 MW; 6D5F7C3D6E4F6304 CRC64;
MSQPIKLLVG LANPGPEYAK TRHNAGAWVV EELARIHNVT LKNEPKFFGL TGRLLINSQE
LRVLIPTTFM NLSGKAIAAL ANFYQIKPEE IMVAHDELDL PPGVAKFKQG GGHGGHNGLK
DTISKLGNNK EFYRLRLGIG HPGHKDKVAG YVLGKAPAKE QECLDAAVDE SVRCLEILMK
DGLTKAQNRL HTFKAE
