PTKA_ECOLI
ID PTKA_ECOLI Reviewed; 150 AA.
AC P69828; P37187; P76413; P94757; P97243;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=PTS system galactitol-specific EIIA component {ECO:0000303|PubMed:7772602};
DE AltName: Full=EIIB-Gat {ECO:0000303|PubMed:7772602};
DE AltName: Full=Galactitol-specific phosphotransferase enzyme IIA component {ECO:0000303|PubMed:7772602};
GN Name=gatA {ECO:0000303|PubMed:7772602};
GN OrderedLocusNames=b2094, JW2078/JW2081;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=EC3132;
RX PubMed=7772602; DOI=10.1016/0167-4781(95)00053-j;
RA Nobelmann B., Lengeler J.W.;
RT "Sequence of the gat operon for galactitol utilization from a wild-type
RT strain EC3132 of Escherichia coli.";
RL Biochim. Biophys. Acta 1262:69-72(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, CATALYTIC ACTIVITY, AND SUBSTRATE SPECIFICITY.
RX PubMed=1100608; DOI=10.1128/jb.124.1.39-47.1975;
RA Lengeler J.;
RT "Nature and properties of hexitol transport systems in Escherichia coli.";
RL J. Bacteriol. 124:39-47(1975).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=8955298; DOI=10.1128/jb.178.23.6790-6795.1996;
RA Nobelmann B., Lengeler J.W.;
RT "Molecular analysis of the gat genes from Escherichia coli and of their
RT roles in galactitol transport and metabolism.";
RL J. Bacteriol. 178:6790-6795(1996).
RN [6]
RP SUBUNIT.
RC STRAIN=BL21-DE3;
RX PubMed=16079137; DOI=10.1074/jbc.m506479200;
RA Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L.,
RA von Heijne G., Daley D.O.;
RT "Protein complexes of the Escherichia coli cell envelope.";
RL J. Biol. Chem. 280:34409-34419(2005).
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC system (PTS), a major carbohydrate active transport system, catalyzes
CC the phosphorylation of incoming sugar substrates concomitant with their
CC translocation across the cell membrane. The enzyme II complex composed
CC of GatA, GatB and GatC is involved in galactitol transport. It can also
CC use D-glucitol. {ECO:0000269|PubMed:1100608,
CC ECO:0000269|PubMed:8955298}.
CC -!- SUBUNIT: Forms a complex with one each of subunit of GatA, GatB and 2
CC subunits of GatC. {ECO:0000269|PubMed:16079137}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:8955298}.
CC -!- INDUCTION: Constitutively expressed. {ECO:0000269|PubMed:8955298}.
CC -!- DOMAIN: The EIIA domain is phosphorylated by phospho-HPr on a histidyl
CC residue. Then, it transfers the phosphoryl group to the EIIB domain.
CC {ECO:0000255|PROSITE-ProRule:PRU00417}.
CC -!- CAUTION: In strain W3110, the sequence is interrupted by the insertion
CC of an IS5 element between positions 69 and 70. {ECO:0000305}.
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DR EMBL; X79837; CAA56228.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75155.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15964.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP009048; BAA15957.2; ALT_SEQ; Genomic_DNA.
DR PIR; E64976; E64976.
DR PIR; S55903; S55903.
DR RefSeq; NP_416597.1; NC_000913.3.
DR RefSeq; WP_000182899.1; NZ_SSZK01000011.1.
DR AlphaFoldDB; P69828; -.
DR SMR; P69828; -.
DR ComplexPortal; CPX-5942; Galactitol-specific enzyme II complex.
DR DIP; DIP-47900N; -.
DR IntAct; P69828; 3.
DR STRING; 511145.b2094; -.
DR TCDB; 4.A.5.1.1; the pts galactitol (gat) family.
DR jPOST; P69828; -.
DR PaxDb; P69828; -.
DR PRIDE; P69828; -.
DR EnsemblBacteria; AAC75155; AAC75155; b2094.
DR EnsemblBacteria; BAA15957; BAA15957; BAA15957.
DR EnsemblBacteria; BAA15964; BAA15964; BAA15964.
DR GeneID; 66674010; -.
DR GeneID; 946633; -.
DR KEGG; ecj:JW2078; -.
DR KEGG; ecj:JW2081; -.
DR KEGG; eco:b2094; -.
DR PATRIC; fig|1411691.4.peg.156; -.
DR EchoBASE; EB2313; -.
DR eggNOG; COG1762; Bacteria.
DR HOGENOM; CLU_2218929_0_0_6; -.
DR OMA; FADYHEA; -.
DR PhylomeDB; P69828; -.
DR BioCyc; EcoCyc:GATA-MON; -.
DR BioCyc; MetaCyc:MON-124140; -.
DR BRENDA; 2.7.1.200; 2026.
DR PRO; PR:P69828; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:1902495; C:transmembrane transporter complex; IC:ComplexPortal.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0030295; F:protein kinase activator activity; IBA:GO_Central.
DR GO; GO:0090584; F:protein-phosphocysteine-galactitol-phosphotransferase system transporter activity; IDA:EcoCyc.
DR GO; GO:0019402; P:galactitol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0015796; P:galactitol transmembrane transport; IDA:EcoCyc.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IDA:EcoCyc.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0032412; P:regulation of ion transmembrane transporter activity; IBA:GO_Central.
DR CDD; cd00211; PTS_IIA_fru; 1.
DR Gene3D; 3.40.930.10; -; 1.
DR InterPro; IPR016152; PTrfase/Anion_transptr.
DR InterPro; IPR002178; PTS_EIIA_type-2_dom.
DR Pfam; PF00359; PTS_EIIA_2; 1.
DR SUPFAM; SSF55804; SSF55804; 1.
DR PROSITE; PS51094; PTS_EIIA_TYPE_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Galactitol metabolism; Kinase; Phosphoprotein;
KW Phosphotransferase system; Reference proteome; Sugar transport;
KW Transferase; Transport.
FT CHAIN 1..150
FT /note="PTS system galactitol-specific EIIA component"
FT /id="PRO_0000186576"
FT DOMAIN 1..144
FT /note="PTS EIIA type-2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00417"
FT ACT_SITE 62
FT /note="Tele-phosphohistidine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00417"
FT MOD_RES 62
FT /note="Phosphohistidine; by HPr"
FT /evidence="ECO:0000305"
FT CONFLICT 124
FT /note="D -> E (in Ref. 1; CAA56228)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 150 AA; 16907 MW; 1A760676BE5FB61D CRC64;
MTNLFVRSGI SFVDRSEVLT HIGNEMLAKG VVHDTWPQAL IAREAEFPTG IMLEQHAIAI
PHCEAIHAKS SAIYLLRPTN KVHFQQADDD NDVAVSLVIA LIVENPQQQL KLLRCLFGKL
QQPDIVETLI TLPETQLKEY FTKYVLDSDE
