PTKB_ECO57
ID PTKB_ECO57 Reviewed; 94 AA.
AC P0A436; Q8X7H5;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=PTS system galactitol-specific EIIB component {ECO:0000250|UniProtKB:P37188};
DE AltName: Full=EIIB-Gat {ECO:0000250|UniProtKB:P37188};
DE AltName: Full=Galactitol-specific phosphotransferase enzyme IIB component {ECO:0000250|UniProtKB:P37188};
DE EC=2.7.1.200 {ECO:0000250|UniProtKB:P37188};
GN Name=gatB; OrderedLocusNames=Z3256, ECs2896;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC system (PTS), a major carbohydrate active transport system, catalyzes
CC the phosphorylation of incoming sugar substrates concomitant with their
CC translocation across the cell membrane. The enzyme II complex composed
CC of GatA, GatB and GatC is involved in galactitol transport.
CC {ECO:0000250|UniProtKB:P37188}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=galactitol(out) + N(pros)-phospho-L-histidyl-[protein] =
CC galactitol 1-phosphate(in) + L-histidyl-[protein];
CC Xref=Rhea:RHEA:49248, Rhea:RHEA-COMP:9745, Rhea:RHEA-COMP:9746,
CC ChEBI:CHEBI:16813, ChEBI:CHEBI:29979, ChEBI:CHEBI:60083,
CC ChEBI:CHEBI:64837; EC=2.7.1.200;
CC Evidence={ECO:0000250|UniProtKB:P37188};
CC -!- SUBUNIT: Forms a complex with one each of subunit of GatA, GatB and 2
CC subunits of GatC. {ECO:0000250|UniProtKB:P37188}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Constitutively expressed. {ECO:0000250|UniProtKB:P37188}.
CC -!- DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a
CC cysteinyl or histidyl residue, depending on the transported sugar.
CC Then, it transfers the phosphoryl group to the sugar substrate
CC concomitantly with the sugar uptake processed by the EIIC domain.
CC {ECO:0000255|PROSITE-ProRule:PRU00422}.
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DR EMBL; AE005174; AAG57150.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB36319.1; -; Genomic_DNA.
DR PIR; B85836; B85836.
DR PIR; H90990; H90990.
DR RefSeq; NP_310923.1; NC_002695.1.
DR RefSeq; WP_000823288.1; NZ_SEKU01000011.1.
DR AlphaFoldDB; P0A436; -.
DR BMRB; P0A436; -.
DR SMR; P0A436; -.
DR STRING; 155864.EDL933_3162; -.
DR EnsemblBacteria; AAG57150; AAG57150; Z3256.
DR EnsemblBacteria; BAB36319; BAB36319; ECs_2896.
DR GeneID; 58389578; -.
DR GeneID; 916598; -.
DR KEGG; ece:Z3256; -.
DR KEGG; ecs:ECs_2896; -.
DR PATRIC; fig|386585.9.peg.3028; -.
DR eggNOG; COG3414; Bacteria.
DR HOGENOM; CLU_159248_3_3_6; -.
DR OMA; AHLICTT; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR GO; GO:0019402; P:galactitol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR InterPro; IPR036095; PTS_EIIB-like_sf.
DR InterPro; IPR013011; PTS_EIIB_2.
DR InterPro; IPR003501; PTS_EIIB_2/3.
DR Pfam; PF02302; PTS_IIB; 1.
DR SUPFAM; SSF52794; SSF52794; 1.
DR PROSITE; PS51099; PTS_EIIB_TYPE_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Galactitol metabolism; Phosphoprotein;
KW Phosphotransferase system; Reference proteome; Sugar transport;
KW Transferase; Transport.
FT CHAIN 1..94
FT /note="PTS system galactitol-specific EIIB component"
FT /id="PRO_0000186574"
FT DOMAIN 1..94
FT /note="PTS EIIB type-2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00422"
FT ACT_SITE 9
FT /note="Phosphocysteine intermediate; for EIIB activity"
FT /evidence="ECO:0000305"
FT MOD_RES 9
FT /note="Phosphocysteine; by EIIA"
FT /evidence="ECO:0000305"
SQ SEQUENCE 94 AA; 10195 MW; 44D4988E0ACACCCF CRC64;
MKRKIIVACG GAVATSTMAA EEIKELCQSH NIPVELIQCR VNEIETYMDG VHLICTTARV
DRSFGDIPLV HGMPFVSGVG IEALQNKILT ILQG
