PTKC_ECOLI
ID PTKC_ECOLI Reviewed; 451 AA.
AC P69831; P37189; P76411;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=PTS system galactitol-specific EIIC component {ECO:0000303|PubMed:7772602};
DE AltName: Full=EIIC-Gat {ECO:0000303|PubMed:7772602};
DE AltName: Full=Galactitol permease IIC component {ECO:0000303|PubMed:7772602};
GN Name=gatC; OrderedLocusNames=b2092, JW2076;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=EC3132;
RX PubMed=7772602; DOI=10.1016/0167-4781(95)00053-j;
RA Nobelmann B., Lengeler J.W.;
RT "Sequence of the gat operon for galactitol utilization from a wild-type
RT strain EC3132 of Escherichia coli.";
RL Biochim. Biophys. Acta 1262:69-72(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP SEQUENCE REVISION.
RC STRAIN=K12 / MG1655 / ATCC 700926;
RX PubMed=22081388; DOI=10.1128/jb.06087-11;
RA Freddolino P.L., Amini S., Tavazoie S.;
RT "Newly identified genetic variations in common Escherichia coli MG1655
RT stock cultures.";
RL J. Bacteriol. 194:303-306(2012).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=BL21-DE3;
RX PubMed=16079137; DOI=10.1074/jbc.m506479200;
RA Stenberg F., Chovanec P., Maslen S.L., Robinson C.V., Ilag L.,
RA von Heijne G., Daley D.O.;
RT "Protein complexes of the Escherichia coli cell envelope.";
RL J. Biol. Chem. 280:34409-34419(2005).
RN [7]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [8]
RP FUNCTION, AND INDUCTION.
RX PubMed=8955298; DOI=10.1128/jb.178.23.6790-6795.1996;
RA Nobelmann B., Lengeler J.W.;
RT "Molecular analysis of the gat genes from Escherichia coli and of their
RT roles in galactitol transport and metabolism.";
RL J. Bacteriol. 178:6790-6795(1996).
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC system (PTS), a major carbohydrate active transport system, catalyzes
CC the phosphorylation of incoming sugar substrates concomitant with their
CC translocation across the cell membrane. The enzyme II complex composed
CC of GatA, GatB and GatC is involved in galactitol transport.
CC {ECO:0000269|PubMed:8955298}.
CC -!- SUBUNIT: Forms a complex with one each of subunit of GatA, GatB and 2
CC subunits of GatC. {ECO:0000269|PubMed:16079137}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00427, ECO:0000269|PubMed:15919996,
CC ECO:0000269|PubMed:16079137}; Multi-pass membrane protein
CC {ECO:0000255|PROSITE-ProRule:PRU00427, ECO:0000269|PubMed:15919996,
CC ECO:0000269|PubMed:16079137}.
CC -!- INDUCTION: Constitutively expressed. {ECO:0000269|PubMed:8955298}.
CC -!- DOMAIN: The EIIC domain forms the PTS system translocation channel and
CC contains the specific substrate-binding site. {ECO:0000255|PROSITE-
CC ProRule:PRU00427}.
CC -!- CAUTION: A pseudogene in strain MG1655 / ATCC 700926 due to a recent
CC laboratory-derived single nucleotide insertion, but not its parent
CC MG1655 / ATCC 47076. {ECO:0000305|PubMed:22081388}.
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DR EMBL; X79837; CAA56230.1; -; Genomic_DNA.
DR EMBL; U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AP009048; BAA15955.1; -; Genomic_DNA.
DR PIR; C64976; C64976.
DR PIR; S55905; S55905.
DR RefSeq; WP_000490679.1; NZ_STEB01000002.1.
DR AlphaFoldDB; P69831; -.
DR BioGRID; 4260430; 14.
DR ComplexPortal; CPX-5942; Galactitol-specific enzyme II complex.
DR STRING; 316407.1736809; -.
DR TCDB; 4.A.5.1.1; the pts galactitol (gat) family.
DR jPOST; P69831; -.
DR PaxDb; P69831; -.
DR PRIDE; P69831; -.
DR EnsemblBacteria; BAA15955; BAA15955; BAA15955.
DR KEGG; ecj:JW2076; -.
DR PATRIC; fig|1411691.4.peg.158; -.
DR EchoBASE; EB2315; -.
DR eggNOG; COG3775; Bacteria.
DR HOGENOM; CLU_040393_0_0_6; -.
DR InParanoid; P69831; -.
DR OMA; VFDMGWP; -.
DR PhylomeDB; P69831; -.
DR BioCyc; MetaCyc:MON-124142; -.
DR BRENDA; 2.7.1.200; 2026.
DR PRO; PR:P69831; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:1902495; C:transmembrane transporter complex; IC:ComplexPortal.
DR GO; GO:0015577; F:galactitol transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0019402; P:galactitol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0015796; P:galactitol transmembrane transport; IC:ComplexPortal.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IC:ComplexPortal.
DR InterPro; IPR013853; GatC.
DR InterPro; IPR013014; PTS_EIIC_2.
DR InterPro; IPR004703; PTS_sugar-sp_permease.
DR PANTHER; PTHR37324; PTHR37324; 1.
DR Pfam; PF03611; EIIC-GAT; 1.
DR PIRSF; PIRSF006304; GatC; 1.
DR TIGRFAMs; TIGR00827; EIIC-GAT; 1.
DR PROSITE; PS51104; PTS_EIIC_TYPE_2; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Galactitol metabolism; Membrane;
KW Phosphotransferase system; Reference proteome; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..451
FT /note="PTS system galactitol-specific EIIC component"
FT /id="PRO_0000186570"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00427"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00427"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00427"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00427"
FT TRANSMEM 218..238
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00427"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00427"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00427"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00427"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00427"
FT TRANSMEM 415..435
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00427"
FT DOMAIN 6..435
FT /note="PTS EIIC type-2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00427"
FT CONFLICT 187..203
FT /note="MGPIAVLVDAIIEKIPG -> LGPNCGAGGCYHRENPS (in Ref. 1;
FT CAA56230)"
FT /evidence="ECO:0000305"
FT CONFLICT 399..451
FT /note="QGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
FT E -> TGRFSHYLVTDSGFSPQIFPVSLLSAQFI (in Ref. 1; CAA56230)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 451 AA; 48365 MW; B843BB6BDE721F13 CRC64;
MFSEVMRYIL DLGPTVMLPI VIIIFSKILG MKAGDCFKAG LHIGIGFVGI GLVIGLMLDS
IGPAAKAMAE NFDLNLHVVD VGWPGSSPMT WASQIALVAI PIAILVNVAM LLTRMTRVVN
VDIWNIWHMT FTGALLHLAT GSWMIGMAGV VIHAAFVYKL GDWFARDTRN FFELEGIAIP
HGTSAYMGPI AVLVDAIIEK IPGVNRIKFS ADDIQRKFGP FGEPVTVGFV MGLIIGILAG
YDVKGVLQLA VKTAAVMLLM PRVIKPIMDG LTPIAKQARS RLQAKFGGQE FLIGLDPALL
LGHTAVVSAS LIFIPLTILI AVCVPGNQVL PFGDLATIGF FVAMAVAVHR GNLFRTLISG
VIIMSITLWI ATQTIGLHTQ LAANAGALKA GGMVASMDQG GSPITWLLIQ VFSPQNIPGF
IIIGAIYLTG IFMTWRRARG FIKQEKVVLA E
