ID   PTLB_BORPE              Reviewed;         104 AA.
AC   Q45391;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Type IV secretion system protein PtlB;
DE   AltName: Full=Pertussis toxin liberation protein B;
GN   Name=ptlB; OrderedLocusNames=BP3789;
OS   Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Bordetella.
OX   NCBI_TaxID=257313;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BP165;
RX   PubMed=2873570; DOI=10.1073/pnas.83.13.4631;
RA   Nicosia A., Perugini M., Franzini C., Casagli M.C., Borri M.G., Antoni G.,
RA   Almoni M., Neri P., Ratti G., Rappuoli R.;
RT   "Cloning and sequencing of the pertussis toxin genes: operon structure and
RT   gene duplication.";
RL   Proc. Natl. Acad. Sci. U.S.A. 83:4631-4635(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=Tohama I / BP338;
RX   PubMed=8464913; DOI=10.1073/pnas.90.7.2970;
RA   Weiss A.A., Johnson F.D., Burns D.L.;
RT   "Molecular characterization of an operon required for pertussis toxin
RT   secretion.";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:2970-2974(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tohama I / ATCC BAA-589 / NCTC 13251;
RX   PubMed=12910271; DOI=10.1038/ng1227;
RA   Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R.,
RA   Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L.,
RA   Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A.,
RA   Achtman M., Atkin R., Baker S., Basham D., Bason N., Cherevach I.,
RA   Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T.,
RA   Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S.,
RA   Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E.,
RA   Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M.,
RA   Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S.,
RA   Barrell B.G., Maskell D.J.;
RT   "Comparative analysis of the genome sequences of Bordetella pertussis,
RT   Bordetella parapertussis and Bordetella bronchiseptica.";
RL   Nat. Genet. 35:32-40(2003).
RN   [4]
RP   REGULATION BY BVGS/BVGA.
RC   STRAIN=Tohama I / ATCC BAA-589 / NCTC 13251;
RX   PubMed=7592424; DOI=10.1128/jb.177.22.6486-6491.1995;
RA   Boucher P.E., Stibitz S.;
RT   "Synergistic binding of RNA polymerase and BvgA phosphate to the pertussis
RT   toxin promoter of Bordetella pertussis.";
RL   J. Bacteriol. 177:6486-6491(1995).
RN   [5]
RP   COTRANSCRIPTION WITH PTX.
RC   STRAIN=Wellcome 28;
RX   PubMed=8606119; DOI=10.1128/iai.64.4.1458-1460.1996;
RA   Ricci S., Rappuoli R., Scarlato V.;
RT   "The pertussis toxin liberation genes of Bordetella pertussis are
RT   transcriptionally linked to the pertussis toxin operon.";
RL   Infect. Immun. 64:1458-1460(1996).
RN   [6]
RP   FUNCTION.
RC   STRAIN=Tohama I / BP338;
RX   PubMed=10518754; DOI=10.1111/j.1574-6968.1999.tb08766.x;
RA   Craig-Mylius K.A., Weiss A.A.;
RT   "Mutants in the ptlA-H genes of Bordetella pertussis are deficient for
RT   pertussis toxin secretion.";
RL   FEMS Microbiol. Lett. 179:479-484(1999).
RN   [7]
RP   FUNCTION.
RC   STRAIN=Tohama I / BP338;
RX   PubMed=15322034; DOI=10.1128/iai.72.9.5365-5372.2004;
RA   Burns D.L., Fiddner S., Cheung A.M., Verma A.;
RT   "Analysis of subassemblies of pertussis toxin subunits in vivo and their
RT   interaction with the ptl transport apparatus.";
RL   Infect. Immun. 72:5365-5372(2004).
CC   -!- FUNCTION: Component of the type IV secretion system ptl required for
CC       secretion of assembled pertussis toxin (PTX) through the outer
CC       membrane. {ECO:0000269|PubMed:10518754, ECO:0000269|PubMed:15322034,
CC       ECO:0000269|PubMed:8464913}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Cotranscribed with ptxABCDE. Activated by the two-component
CC       regulatory system BvgS/BvgA.
CC   -!- SIMILARITY: Belongs to the virB3 family. {ECO:0000305}.
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DR   EMBL; M14378; AAA83986.1; -; Genomic_DNA.
DR   EMBL; L10720; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BX640422; CAE44044.1; -; Genomic_DNA.
DR   PIR; A47301; A47301.
DR   RefSeq; NP_882288.1; NC_002929.2.
DR   RefSeq; WP_010929494.1; NZ_CP039022.1.
DR   AlphaFoldDB; Q45391; -.
DR   STRING; 257313.BP3789; -.
DR   TCDB; 3.A.7.3.1; the type iv (conjugal dna-protein transfer or virb) secretory pathway (ivsp) family.
DR   GeneID; 66440814; -.
DR   KEGG; bpe:BP3789; -.
DR   PATRIC; fig|257313.5.peg.4093; -.
DR   eggNOG; COG3702; Bacteria.
DR   HOGENOM; CLU_158477_0_0_4; -.
DR   OMA; WIDTKFR; -.
DR   Proteomes; UP000002676; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR007792; T4SS_VirB3/TrbD/AvhB.
DR   Pfam; PF05101; VirB3; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..104
FT                   /note="Type IV secretion system protein PtlB"
FT                   /id="PRO_0000262573"
FT   TRANSMEM        30..50
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   104 AA;  12312 MW;  581DD0CA8173B4B4 CRC64;
     MRDPLFKGCT RPAMLMGVPA TPLAVCSGTI ALLGIWFSIA FLALFPVALL AMRIMIRRDD
     QQFRLIWLYL RMRWLSRDRT HAFWQSTVYA PLRYAERRRR LRKP