ID   PTLE_BORBR              Reviewed;         233 AA.
AC   Q7WDT8;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Type IV secretion system protein PtlE homolog;
GN   Name=ptlE; OrderedLocusNames=BB4899;
OS   Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50)
OS   (Alcaligenes bronchisepticus).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Bordetella.
OX   NCBI_TaxID=257310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-588 / NCTC 13252 / RB50;
RX   PubMed=12910271; DOI=10.1038/ng1227;
RA   Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R.,
RA   Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L.,
RA   Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A.,
RA   Achtman M., Atkin R., Baker S., Basham D., Bason N., Cherevach I.,
RA   Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T.,
RA   Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S.,
RA   Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E.,
RA   Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M.,
RA   Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S.,
RA   Barrell B.G., Maskell D.J.;
RT   "Comparative analysis of the genome sequences of Bordetella pertussis,
RT   Bordetella parapertussis and Bordetella bronchiseptica.";
RL   Nat. Genet. 35:32-40(2003).
RN   [2]
RP   TRANSCRIPTIONAL SILENCING.
RC   STRAIN=ATCC 4617 / NCIB 9935 / NCTC 8344 / NRRL B-140;
RX   PubMed=3584073; DOI=10.1128/jb.169.6.2847-2853.1987;
RA   Arico B., Rappuoli R.;
RT   "Bordetella parapertussis and Bordetella bronchiseptica contain
RT   transcriptionally silent pertussis toxin genes.";
RL   J. Bacteriol. 169:2847-2853(1987).
RN   [3]
RP   IN VITRO EXPRESSION FROM A B.PERTUSSIS PROMOTER.
RC   STRAIN=ATCC 31437 / Bb55;
RX   PubMed=8926063; DOI=10.1128/iai.64.10.4020-4026.1996;
RA   Hausman S.Z., Cherry J.D., Heininger U., Wirsing von Koenig C.H.,
RA   Burns D.L.;
RT   "Analysis of proteins encoded by the ptx and ptl genes of Bordetella
RT   bronchiseptica and Bordetella parapertussis.";
RL   Infect. Immun. 64:4020-4026(1996).
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}.
CC   -!- MISCELLANEOUS: B.bronchiseptica strain Bb55 harboring a functional ptx-
CC       ptl promoter from B.pertussis produces the PtlE protein in a stable
CC       form. Is also able to produce and secrete efficiently the pertussis
CC       toxin (PTX).
CC   -!- SIMILARITY: Belongs to the virB8 family. {ECO:0000305}.
CC   -!- CAUTION: B.parapertussis and B.bronchiseptica seem not to produce the
CC       pertussis toxin (S1, S2, S4, S5 and S3) and ptl proteins (PtlA, PtlB,
CC       PtlC, PtlD, PtlE, PtlF, PtlG, PtlH and PtlI) in vivo due to changes in
CC       the promoter region of the ptx-ptl operon. However, it is possible that
CC       their promoter is active under certain, as-yet-undefined conditions and
CC       that B.parapertussis and B.bronchiseptica are therefore capable of
CC       producing these proteins. {ECO:0000305}.
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DR   EMBL; BX640451; CAE35263.1; -; Genomic_DNA.
DR   RefSeq; WP_010927236.1; NC_002927.3.
DR   AlphaFoldDB; Q7WDT8; -.
DR   SMR; Q7WDT8; -.
DR   STRING; 257310.BB4899; -.
DR   EnsemblBacteria; CAE35263; CAE35263; BB4899.
DR   GeneID; 56476601; -.
DR   KEGG; bbr:BB4899; -.
DR   eggNOG; COG3736; Bacteria.
DR   HOGENOM; CLU_068461_1_1_4; -.
DR   OMA; DLIQVRY; -.
DR   OrthoDB; 887933at2; -.
DR   Proteomes; UP000001027; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030255; P:protein secretion by the type IV secretion system; IEA:InterPro.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR007430; VirB8.
DR   InterPro; IPR026264; VirB8/PtlE.
DR   Pfam; PF04335; VirB8; 1.
DR   PIRSF; PIRSF003299; VirB8_PtlE; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..233
FT                   /note="Type IV secretion system protein PtlE homolog"
FT                   /id="PRO_0000287411"
FT   TRANSMEM        42..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   233 AA;  26022 MW;  10DB9F23B5E4BE31 CRC64;
     MPDPRPLTPD QTHGRGHAEA AVDWEASRLY RLAQSERRAW TVAWAALAVT ALSLIAIATM
     LPLKTTIPYL IEVEKSSGAA SVVTQFEPRD FTPDTLMNQY WLTRYVAARE RYDWHTIQHD
     YDYVRLLSAP AVRHDYETSY EAPDAPDRKY GAGTTLAVKI LSAIDHGKGV GTVRFVRTRR
     DADGQGAAES SIWVATVAFA YDRPRALTQA QRWLNPLGFA VTSYRVDAEA GQP