ID   PTLF_BORBR              Reviewed;         273 AA.
AC   Q7WDT7;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Type IV secretion system protein PtlF homolog;
DE   Flags: Precursor;
GN   Name=ptlF; OrderedLocusNames=BB4900;
OS   Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50)
OS   (Alcaligenes bronchisepticus).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Bordetella.
OX   NCBI_TaxID=257310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-588 / NCTC 13252 / RB50;
RX   PubMed=12910271; DOI=10.1038/ng1227;
RA   Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R.,
RA   Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L.,
RA   Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A.,
RA   Achtman M., Atkin R., Baker S., Basham D., Bason N., Cherevach I.,
RA   Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T.,
RA   Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S.,
RA   Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E.,
RA   Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M.,
RA   Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S.,
RA   Barrell B.G., Maskell D.J.;
RT   "Comparative analysis of the genome sequences of Bordetella pertussis,
RT   Bordetella parapertussis and Bordetella bronchiseptica.";
RL   Nat. Genet. 35:32-40(2003).
RN   [2]
RP   TRANSCRIPTIONAL SILENCING.
RC   STRAIN=ATCC 4617 / NCIB 9935 / NCTC 8344 / NRRL B-140;
RX   PubMed=3584073; DOI=10.1128/jb.169.6.2847-2853.1987;
RA   Arico B., Rappuoli R.;
RT   "Bordetella parapertussis and Bordetella bronchiseptica contain
RT   transcriptionally silent pertussis toxin genes.";
RL   J. Bacteriol. 169:2847-2853(1987).
RN   [3]
RP   IN VITRO EXPRESSION FROM A B.PERTUSSIS PROMOTER.
RC   STRAIN=ATCC 31437 / Bb55;
RX   PubMed=8926063; DOI=10.1128/iai.64.10.4020-4026.1996;
RA   Hausman S.Z., Cherry J.D., Heininger U., Wirsing von Koenig C.H.,
RA   Burns D.L.;
RT   "Analysis of proteins encoded by the ptx and ptl genes of Bordetella
RT   bronchiseptica and Bordetella parapertussis.";
RL   Infect. Immun. 64:4020-4026(1996).
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}.
CC   -!- MISCELLANEOUS: B.bronchiseptica strain Bb55 harboring a functional ptx-
CC       ptl promoter from B.pertussis produces the PtlF protein in a stable
CC       form. Is also able to produce and secrete efficiently the pertussis
CC       toxin (PTX).
CC   -!- SIMILARITY: Belongs to the TrbG/VirB9 family. {ECO:0000305}.
CC   -!- CAUTION: B.parapertussis and B.bronchiseptica seem not to produce the
CC       pertussis toxin (S1, S2, S4, S5 and S3) and ptl proteins (PtlA, PtlB,
CC       PtlC, PtlD, PtlE, PtlF, PtlG, PtlH and PtlI) in vivo due to changes in
CC       the promoter region of the ptx-ptl operon. However, it is possible that
CC       their promoter is active under certain, as-yet-undefined conditions and
CC       that B.parapertussis and B.bronchiseptica are therefore capable of
CC       producing these proteins. {ECO:0000305}.
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DR   EMBL; BX640451; CAE35264.1; -; Genomic_DNA.
DR   RefSeq; WP_003815863.1; NC_002927.3.
DR   AlphaFoldDB; Q7WDT7; -.
DR   SMR; Q7WDT7; -.
DR   STRING; 257310.BB4900; -.
DR   EnsemblBacteria; CAE35264; CAE35264; BB4900.
DR   GeneID; 56476600; -.
DR   GeneID; 66440819; -.
DR   KEGG; bbr:BB4900; -.
DR   eggNOG; COG3504; Bacteria.
DR   HOGENOM; CLU_058585_3_0_4; -.
DR   OMA; GFYGYQS; -.
DR   OrthoDB; 1251301at2; -.
DR   Proteomes; UP000001027; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   CDD; cd06911; VirB9_CagX_TrbG; 1.
DR   Gene3D; 2.60.40.2500; -; 1.
DR   InterPro; IPR010258; Conjugal_tfr_TrbG/VirB9/CagX.
DR   InterPro; IPR014148; VirB9.
DR   InterPro; IPR033645; VirB9/CagX/TrbG_C.
DR   InterPro; IPR038161; VirB9/CagX/TrbG_C_sf.
DR   Pfam; PF03524; CagX; 1.
DR   TIGRFAMs; TIGR02781; VirB9; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Membrane; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..273
FT                   /note="Type IV secretion system protein PtlF homolog"
FT                   /id="PRO_0000287413"
SQ   SEQUENCE   273 AA;  29413 MW;  58EDA354539A3EB0 CRC64;
     MMAARMMAAG LAATALSAHA FRIPTPGEQD ARIQTVPYHP EEVVLVRAWN GYVTRIVFDE
     QEKIIDVAAG FADGWQFSPE GNVLYIKAKS FPAQGSPAQA PEPGLWNTNL LVKTDRRLYD
     FDLVLASADA ATPQALQRSR MAYRLQFRYP AAPQAASRAS PVGPAVPAGA LNRRYAMQVG
     NGSDGIAPIA AYDDGRHTWL TFRPGQPFPA VFAVAPDGTE TLVNLHIDNQ SLVIHRVAPV
     LMLRSGASVI RIVNQNGDAS ASPAFECHAE PAL