PTLF_BORPE
ID PTLF_BORPE Reviewed; 273 AA.
AC Q7VSX5;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=Type IV secretion system protein PtlF;
DE AltName: Full=Pertussis toxin liberation protein F;
DE Flags: Precursor;
GN Name=ptlF; OrderedLocusNames=BP3794;
OS Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Alcaligenaceae; Bordetella.
OX NCBI_TaxID=257313;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=Tohama I / BP338;
RX PubMed=8464913; DOI=10.1073/pnas.90.7.2970;
RA Weiss A.A., Johnson F.D., Burns D.L.;
RT "Molecular characterization of an operon required for pertussis toxin
RT secretion.";
RL Proc. Natl. Acad. Sci. U.S.A. 90:2970-2974(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tohama I / ATCC BAA-589 / NCTC 13251;
RX PubMed=12910271; DOI=10.1038/ng1227;
RA Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R.,
RA Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L.,
RA Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A.,
RA Achtman M., Atkin R., Baker S., Basham D., Bason N., Cherevach I.,
RA Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T.,
RA Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S.,
RA Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E.,
RA Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M.,
RA Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S.,
RA Barrell B.G., Maskell D.J.;
RT "Comparative analysis of the genome sequences of Bordetella pertussis,
RT Bordetella parapertussis and Bordetella bronchiseptica.";
RL Nat. Genet. 35:32-40(2003).
RN [3]
RP SUBCELLULAR LOCATION.
RC STRAIN=Tohama I / BP338;
RX PubMed=8071211; DOI=10.1128/jb.176.17.5350-5356.1994;
RA Johnson F.D., Burns D.L.;
RT "Detection and subcellular localization of three Ptl proteins involved in
RT the secretion of pertussis toxin from Bordetella pertussis.";
RL J. Bacteriol. 176:5350-5356(1994).
RN [4]
RP REGULATION BY BVGS/BVGA.
RC STRAIN=Tohama I / ATCC BAA-589 / NCTC 13251;
RX PubMed=7592424; DOI=10.1128/jb.177.22.6486-6491.1995;
RA Boucher P.E., Stibitz S.;
RT "Synergistic binding of RNA polymerase and BvgA phosphate to the pertussis
RT toxin promoter of Bordetella pertussis.";
RL J. Bacteriol. 177:6486-6491(1995).
RN [5]
RP COTRANSCRIPTION WITH PTX.
RC STRAIN=Wellcome 28;
RX PubMed=8606119; DOI=10.1128/iai.64.4.1458-1460.1996;
RA Ricci S., Rappuoli R., Scarlato V.;
RT "The pertussis toxin liberation genes of Bordetella pertussis are
RT transcriptionally linked to the pertussis toxin operon.";
RL Infect. Immun. 64:1458-1460(1996).
RN [6]
RP INTERACTION WITH PTLI.
RC STRAIN=Tohama I / BP338;
RX PubMed=8940184; DOI=10.1074/jbc.271.49.31643;
RA Farizo K.M., Cafarella T.G., Burns D.L.;
RT "Evidence for a ninth gene, ptlI, in the locus encoding the pertussis toxin
RT secretion system of Bordetella pertussis and formation of a PtlI-PtlF
RT complex.";
RL J. Biol. Chem. 271:31643-31649(1996).
RN [7]
RP FUNCTION.
RC STRAIN=Tohama I / BP338;
RX PubMed=10518754; DOI=10.1111/j.1574-6968.1999.tb08766.x;
RA Craig-Mylius K.A., Weiss A.A.;
RT "Mutants in the ptlA-H genes of Bordetella pertussis are deficient for
RT pertussis toxin secretion.";
RL FEMS Microbiol. Lett. 179:479-484(1999).
RN [8]
RP FUNCTION.
RC STRAIN=Tohama I / BP338;
RX PubMed=15322034; DOI=10.1128/iai.72.9.5365-5372.2004;
RA Burns D.L., Fiddner S., Cheung A.M., Verma A.;
RT "Analysis of subassemblies of pertussis toxin subunits in vivo and their
RT interaction with the ptl transport apparatus.";
RL Infect. Immun. 72:5365-5372(2004).
CC -!- FUNCTION: Component of the type IV secretion system ptl required for
CC secretion of assembled pertussis toxin (PTX) through the outer
CC membrane. {ECO:0000269|PubMed:10518754, ECO:0000269|PubMed:15322034,
CC ECO:0000269|PubMed:8464913}.
CC -!- SUBUNIT: Forms a complex with PtlI.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}.
CC -!- INDUCTION: Cotranscribed with ptxABCDE. Activated by the two-component
CC regulatory system BvgS/BvgA.
CC -!- SIMILARITY: Belongs to the TrbG/VirB9 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L10720; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BX640422; CAE44049.1; -; Genomic_DNA.
DR RefSeq; NP_882293.1; NC_002929.2.
DR RefSeq; WP_010931654.1; NZ_CP039022.1.
DR AlphaFoldDB; Q7VSX5; -.
DR SMR; Q7VSX5; -.
DR STRING; 257313.BP3794; -.
DR GeneID; 45390940; -.
DR KEGG; bpe:BP3794; -.
DR PATRIC; fig|257313.5.peg.4098; -.
DR eggNOG; COG3504; Bacteria.
DR HOGENOM; CLU_058585_3_0_4; -.
DR OMA; GFYGYQS; -.
DR Proteomes; UP000002676; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR CDD; cd06911; VirB9_CagX_TrbG; 1.
DR Gene3D; 2.60.40.2500; -; 1.
DR InterPro; IPR010258; Conjugal_tfr_TrbG/VirB9/CagX.
DR InterPro; IPR014148; VirB9.
DR InterPro; IPR033645; VirB9/CagX/TrbG_C.
DR InterPro; IPR038161; VirB9/CagX/TrbG_C_sf.
DR Pfam; PF03524; CagX; 1.
DR TIGRFAMs; TIGR02781; VirB9; 1.
PE 1: Evidence at protein level;
KW Cell outer membrane; Membrane; Reference proteome; Signal; Transport.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..273
FT /note="Type IV secretion system protein PtlF"
FT /id="PRO_0000262577"
SQ SEQUENCE 273 AA; 29471 MW; 58EDA354568E3EB0 CRC64;
MMAARMMAAG LAATALSAHA FRIPTPGEQD ARIQTVPYHP EEVVLVRAWN GYVTRIVFDE
QEKIIDVAAG FADGWQFSPE GNVLYIKAKS FPAQGSPAQA PEPGLWNTNL LVKTDRRLYD
FDLVLASADA ATPQALQRSR MAYRLQFRYP AAPQAASRAS PVGPAVPAGA LNRRYAMQVG
NGSDGIAPIA AYDDGRHTWL TFRPGQPFPA VFAVAPDGTE TLVNLHIDNQ SLVIHRVAPV
LMLRSGASVI RIVNQNGDAS ESPAFECHAE PAL
