PTL_ARATH
ID PTL_ARATH Reviewed; 591 AA.
AC Q9LZS0; Q6Q6T0;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Trihelix transcription factor PTL;
DE AltName: Full=Trihelix DNA-binding protein PETAL LOSS;
GN Name=PTL; OrderedLocusNames=At5g03680; ORFNames=F17C15.100;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION BY PINOID.
RC STRAIN=cv. C24, cv. Columbia, and cv. Landsberg erecta; TISSUE=Flower;
RX PubMed=15269176; DOI=10.1242/dev.01279;
RA Brewer P.B., Howles P.A., Dorian K., Griffith M.E., Ishida T.,
RA Kaplan-Levy R.N., Kilinc A., Smyth D.R.;
RT "PETAL LOSS, a trihelix transcription factor gene, regulates perianth
RT architecture in the Arabidopsis flower.";
RL Development 131:4035-4045(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. C24, and cv. Columbia;
RX PubMed=10572040; DOI=10.1242/dev.126.24.5635;
RA Griffith M.E., da Silva Conceicao A., Smyth D.R.;
RT "PETAL LOSS gene regulates initiation and orientation of second whorl
RT organs in the Arabidopsis flower.";
RL Development 126:5635-5644(1999).
RN [5]
RP FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=18080804; DOI=10.1007/s11103-007-9272-6;
RA Li X., Qin G., Chen Z., Gu H., Qu L.-J.;
RT "A gain-of-function mutation of transcriptional factor PTL results in curly
RT leaves, dwarfism and male sterility by affecting auxin homeostasis.";
RL Plant Mol. Biol. 66:315-327(2008).
RN [6]
RP INTERACTION WITH KIN10, AND TISSUE SPECIFICITY.
RX PubMed=25697797; DOI=10.1093/jxb/erv032;
RA O'Brien M., Kaplan-Levy R.N., Quon T., Sappl P.G., Smyth D.R.;
RT "PETAL LOSS, a trihelix transcription factor that represses growth in
RT Arabidopsis thaliana, binds the energy-sensing SnRK1 kinase AKIN10.";
RL J. Exp. Bot. 66:2475-2485(2015).
CC -!- FUNCTION: Transcription factor that prevents growth. Regulates perianth
CC architecture in flower, mostly in the second whorl, probably by
CC suppressing growth between initiating sepals, ensuring that they remain
CC separate, and by modulating organ shapes. Required for the
CC establishment of auxin flux. {ECO:0000269|PubMed:10572040,
CC ECO:0000269|PubMed:15269176, ECO:0000269|PubMed:18080804}.
CC -!- SUBUNIT: Interacts with KIN10. {ECO:0000269|PubMed:25697797}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Confined to flowers, at low levels. Also present in
CC 7-days-old seedlings. Barely detectable in other tissues such as young
CC seedlings, roots, stems, leaves and siliques (PubMed:15269176).
CC Expressed in flower primordia, more precisely between newly arisen
CC sepal primordia and also at the basal margins of developing sepals
CC (PubMed:25697797). {ECO:0000269|PubMed:15269176,
CC ECO:0000269|PubMed:25697797}.
CC -!- DEVELOPMENTAL STAGE: First observed in the early-developing flower, in
CC four zones between the initiating sepals and in their developing
CC margins. Later detected in the margins of expanding sepals, petals and
CC stamens, and in the leaf margins. {ECO:0000269|PubMed:15269176}.
CC -!- INDUCTION: Expression within the newly arising sepals is repressed via
CC the PINOID auxin-response pathway. {ECO:0000269|PubMed:15269176}.
CC -!- DISRUPTION PHENOTYPE: Disrupted perianth development, particularly
CC petal initiation and orientation, sometimes leading to petal fusion.
CC {ECO:0000269|PubMed:10572040, ECO:0000269|PubMed:15269176}.
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DR EMBL; AY555728; AAS64746.1; -; mRNA.
DR EMBL; AL162506; CAB82933.1; -; Genomic_DNA.
DR EMBL; CP002688; AED90641.1; -; Genomic_DNA.
DR PIR; T48395; T48395.
DR RefSeq; NP_195988.1; NM_120449.3.
DR AlphaFoldDB; Q9LZS0; -.
DR SMR; Q9LZS0; -.
DR BioGRID; 17033; 3.
DR STRING; 3702.AT5G03680.1; -.
DR PaxDb; Q9LZS0; -.
DR PRIDE; Q9LZS0; -.
DR ProteomicsDB; 226433; -.
DR EnsemblPlants; AT5G03680.1; AT5G03680.1; AT5G03680.
DR GeneID; 831757; -.
DR Gramene; AT5G03680.1; AT5G03680.1; AT5G03680.
DR KEGG; ath:AT5G03680; -.
DR Araport; AT5G03680; -.
DR TAIR; locus:2144553; AT5G03680.
DR eggNOG; KOG4282; Eukaryota.
DR HOGENOM; CLU_035771_0_0_1; -.
DR InParanoid; Q9LZS0; -.
DR OMA; IDKEHLF; -.
DR OrthoDB; 639980at2759; -.
DR PhylomeDB; Q9LZS0; -.
DR PRO; PR:Q9LZS0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LZS0; baseline and differential.
DR Genevisible; Q9LZS0; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0019900; F:kinase binding; IPI:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0048498; P:establishment of petal orientation; IMP:TAIR.
DR GO; GO:0046621; P:negative regulation of organ growth; IMP:TAIR.
DR GO; GO:0090428; P:perianth development; IMP:TAIR.
DR GO; GO:0048441; P:petal development; IMP:TAIR.
DR GO; GO:0009909; P:regulation of flower development; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0048442; P:sepal development; IGI:TAIR.
DR InterPro; IPR044822; Myb_DNA-bind_4.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF13837; Myb_DNA-bind_4; 2.
DR PROSITE; PS50090; MYB_LIKE; 2.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Growth regulation; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation.
FT CHAIN 1..591
FT /note="Trihelix transcription factor PTL"
FT /id="PRO_0000421377"
FT DOMAIN 118..177
FT /note="Myb-like 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00133"
FT DOMAIN 422..479
FT /note="Myb-like 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00133"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 380..410
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 491..551
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 383..410
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 514..551
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 459
FT /note="A -> V (in Ref. 1; AAS64746)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 591 AA; 66639 MW; A9DB191A51128848 CRC64;
MDQDQHPQYG IPELRQLMKG GGRTTTTTPS TSSHFPSDFF GFNLAPVQPP PHRLHQFTTD
QDMGFLPRGI HGLGGGSSTA GNNSNLNAST SGGGVGFSGF LDGGGFGSGV GGDGGGTGRW
PRQETLTLLE IRSRLDHKFK EANQKGPLWD EVSRIMSEEH GYQRSGKKCR EKFENLYKYY
RKTKEGKAGR QDGKHYRFFR QLEALYGDSN NLVSCPNHNT QFMSSALHGF HTQNPMNVTT
TTSNIHNVDS VHGFHQSLSL SNNYNSSELE LMTSSSEGND SSSRRKKRSW KAKIKEFIDT
NMKRLIERQD VWLEKLTKVI EDKEEQRMMK EEEWRKIEAA RIDKEHLFWA KERARMEARD
VAVIEALQYL TGKPLIKPLC SSPEERTNGN NEIRNNSETQ NENGSDQTMT NNVCVKGSSS
CWGEQEILKL MEIRTSMDST FQEILGGCSD EFLWEEIAAK LIQLGFDQRS ALLCKEKWEW
ISNGMRKEKK QINKKRKDNS SSCGVYYPRN EENPIYNNRE SGYNDNDPHQ INEQGNVGSS
TSNANANANV TTGNPSGAMA ASTNCFPFFM GDGDQNLWES YGLRLSKEEN Q
