ID   PTMA_ENTFA              Reviewed;         145 AA.
AC   P27547;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   23-APR-2003, sequence version 2.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Mannitol-specific phosphotransferase enzyme IIA component {ECO:0000250|UniProtKB:P0A0E0};
DE   AltName: Full=EIIA {ECO:0000250|UniProtKB:P0A0E0};
DE   AltName: Full=EIII {ECO:0000303|PubMed:1904856};
DE   AltName: Full=PTS system mannitol-specific EIIA component {ECO:0000250|UniProtKB:P0A0E0};
GN   Name=mtlF {ECO:0000303|PubMed:1904856}; OrderedLocusNames=EF_0412;
OS   Enterococcus faecalis (strain ATCC 700802 / V583).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=226185;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1904856; DOI=10.1128/jb.173.12.3709-3715.1991;
RA   Fischer R., von Strandmann R.P., Hengstenberg W.;
RT   "Mannitol-specific phosphoenolpyruvate-dependent phosphotransferase system
RT   of Enterococcus faecalis: molecular cloning and nucleotide sequences of the
RT   enzyme IIIMtl gene and the mannitol-1-phosphate dehydrogenase gene,
RT   expression in Escherichia coli, and comparison of the gene products with
RT   similar enzymes.";
RL   J. Bacteriol. 173:3709-3715(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700802 / V583;
RX   PubMed=12663927; DOI=10.1126/science.1080613;
RA   Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R.,
RA   Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H.,
RA   Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C.,
RA   DeBoy R.T., Durkin S.A., Kolonay J.F., Madupu R., Nelson W.C.,
RA   Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M.,
RA   Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.;
RT   "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus
RT   faecalis.";
RL   Science 299:2071-2074(2003).
CC   -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC       system (sugar PTS), a major carbohydrate active transport system,
CC       catalyzes the phosphorylation of incoming sugar substrates
CC       concomitantly with their translocation across the cell membrane. The
CC       enzyme II CmtAB PTS system is involved in D-mannitol transport.
CC       {ECO:0000250|UniProtKB:P0A0E0}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- DOMAIN: The PTS EIIA type-2 domain is phosphorylated by phospho-HPr on
CC       a histidyl residue. Then, it transfers the phosphoryl group to the PTS
CC       EIIB type-2 domain. {ECO:0000255|PROSITE-ProRule:PRU00417}.
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DR   EMBL; M38386; AAA24779.1; -; Genomic_DNA.
DR   EMBL; AE016830; AAO80271.1; -; Genomic_DNA.
DR   PIR; A39435; WQSO3M.
DR   RefSeq; NP_814200.1; NC_004668.1.
DR   RefSeq; WP_002355279.1; NZ_KE136524.1.
DR   AlphaFoldDB; P27547; -.
DR   SMR; P27547; -.
DR   STRING; 226185.EF_0412; -.
DR   EnsemblBacteria; AAO80271; AAO80271; EF_0412.
DR   GeneID; 60892856; -.
DR   KEGG; efa:EF0412; -.
DR   PATRIC; fig|226185.45.peg.2920; -.
DR   eggNOG; COG4668; Bacteria.
DR   HOGENOM; CLU_072531_3_0_9; -.
DR   OMA; EDYIQAM; -.
DR   Proteomes; UP000001415; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   CDD; cd00211; PTS_IIA_fru; 1.
DR   Gene3D; 3.40.930.10; -; 1.
DR   InterPro; IPR016152; PTrfase/Anion_transptr.
DR   InterPro; IPR002178; PTS_EIIA_type-2_dom.
DR   Pfam; PF00359; PTS_EIIA_2; 1.
DR   SUPFAM; SSF55804; SSF55804; 1.
DR   PROSITE; PS51094; PTS_EIIA_TYPE_2; 1.
DR   PROSITE; PS00372; PTS_EIIA_TYPE_2_HIS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Kinase; Phosphoprotein; Phosphotransferase system;
KW   Reference proteome; Sugar transport; Transferase; Transport.
FT   CHAIN           1..145
FT                   /note="Mannitol-specific phosphotransferase enzyme IIA
FT                   component"
FT                   /id="PRO_0000186634"
FT   DOMAIN          2..145
FT                   /note="PTS EIIA type-2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00417"
FT   ACT_SITE        62
FT                   /note="Tele-phosphohistidine intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:P0A0E0,
FT                   ECO:0000255|PROSITE-ProRule:PRU00417"
FT   MOD_RES         62
FT                   /note="Phosphohistidine; by HPr"
FT                   /evidence="ECO:0000250|UniProtKB:P00550,
FT                   ECO:0000250|UniProtKB:P0A0E0"
FT   CONFLICT        25..27
FT                   /note="CGE -> SGQ (in Ref. 1; AAA24779)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        52..53
FT                   /note="VY -> AH (in Ref. 1; AAA24779)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   145 AA;  15947 MW;  AD7417F03A191C4C CRC64;
     MENLTNISIE LNQQFNTKEE AIRFCGEKLV EAGCVEPAYI EAMIERDQLL SVYMGNFIAI
     PHGTEEAKKL VKKSGICVVQ VPEGVNFGTE EDEKIATVLF GIAGVGEEHL QLVQQIALYC
     SDMDNVVQLA DALSKEEITE NLAIA