PTN21_MOUSE
ID PTN21_MOUSE Reviewed; 1176 AA.
AC Q62136;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Tyrosine-protein phosphatase non-receptor type 21;
DE EC=3.1.3.48;
DE AltName: Full=Protein-tyrosine phosphatase PTP-RL10;
GN Name=Ptpn21;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J; TISSUE=Liver;
RX PubMed=7838537;
RA Higashitsuji H., Arii S., Furutani M., Imamura M., Kaneko Y., Takenawa J.,
RA Nakayama H., Fujita J.;
RT "Enhanced expression of multiple protein tyrosine phosphatases in the
RT regenerating mouse liver: isolation of PTP-RL10, a novel cytoplasmic-type
RT phosphatase with sequence homology to cytoskeletal protein 4.1.";
RL Oncogene 10:407-414(1995).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-577; SER-637 AND SER-806, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-589; SER-590; SER-637;
RP SER-673; SER-799; SER-801 AND SER-806, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May be involved in the regulation of growth and
CC differentiation of liver cells.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:10684, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC COMP:10137, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:46858,
CC ChEBI:CHEBI:82620; EC=3.1.3.48; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU10044};
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Liver.
CC -!- SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-
CC receptor class subfamily. {ECO:0000305}.
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DR EMBL; D37801; BAA07053.1; -; mRNA.
DR CCDS; CCDS26098.1; -.
DR PIR; I58345; I58345.
DR AlphaFoldDB; Q62136; -.
DR SMR; Q62136; -.
DR STRING; 10090.ENSMUSP00000082197; -.
DR iPTMnet; Q62136; -.
DR PhosphoSitePlus; Q62136; -.
DR jPOST; Q62136; -.
DR MaxQB; Q62136; -.
DR PaxDb; Q62136; -.
DR PRIDE; Q62136; -.
DR ProteomicsDB; 301938; -.
DR MGI; MGI:1344406; Ptpn21.
DR eggNOG; KOG0792; Eukaryota.
DR InParanoid; Q62136; -.
DR PhylomeDB; Q62136; -.
DR ChiTaRS; Ptpn21; mouse.
DR PRO; PR:Q62136; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q62136; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0004725; F:protein tyrosine phosphatase activity; ISO:MGI.
DR GO; GO:0006470; P:protein dephosphorylation; IEA:InterPro.
DR CDD; cd14473; FERM_B-lobe; 1.
DR CDD; cd13188; FERM_C_PTPN14_PTPN21; 1.
DR Gene3D; 1.20.80.10; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR Gene3D; 3.90.190.10; -; 1.
DR InterPro; IPR019749; Band_41_domain.
DR InterPro; IPR014352; FERM/acyl-CoA-bd_prot_sf.
DR InterPro; IPR035963; FERM_2.
DR InterPro; IPR019748; FERM_central.
DR InterPro; IPR019747; FERM_CS.
DR InterPro; IPR000299; FERM_domain.
DR InterPro; IPR018979; FERM_N.
DR InterPro; IPR018980; FERM_PH-like_C.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR029021; Prot-tyrosine_phosphatase-like.
DR InterPro; IPR000242; PTP_cat.
DR InterPro; IPR014392; PTP_non-rcpt_14/21.
DR InterPro; IPR041782; PTPN14/21_FERM_C.
DR InterPro; IPR016130; Tyr_Pase_AS.
DR InterPro; IPR003595; Tyr_Pase_cat.
DR InterPro; IPR000387; Tyr_Pase_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR Pfam; PF09380; FERM_C; 1.
DR Pfam; PF00373; FERM_M; 1.
DR Pfam; PF09379; FERM_N; 1.
DR Pfam; PF00102; Y_phosphatase; 1.
DR PIRSF; PIRSF000934; Tyr-Ptase_nr14; 1.
DR PRINTS; PR00935; BAND41.
DR PRINTS; PR00700; PRTYPHPHTASE.
DR SMART; SM00295; B41; 1.
DR SMART; SM01196; FERM_C; 1.
DR SMART; SM00194; PTPc; 1.
DR SMART; SM00404; PTPc_motif; 1.
DR SUPFAM; SSF47031; SSF47031; 1.
DR SUPFAM; SSF52799; SSF52799; 1.
DR SUPFAM; SSF54236; SSF54236; 1.
DR PROSITE; PS00660; FERM_1; 1.
DR PROSITE; PS00661; FERM_2; 1.
DR PROSITE; PS50057; FERM_3; 1.
DR PROSITE; PS00383; TYR_PHOSPHATASE_1; 1.
DR PROSITE; PS50056; TYR_PHOSPHATASE_2; 1.
DR PROSITE; PS50055; TYR_PHOSPHATASE_PTP; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Hydrolase; Phosphoprotein; Protein phosphatase;
KW Reference proteome.
FT CHAIN 1..1176
FT /note="Tyrosine-protein phosphatase non-receptor type 21"
FT /id="PRO_0000219440"
FT DOMAIN 23..308
FT /note="FERM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00084"
FT DOMAIN 898..1169
FT /note="Tyrosine-protein phosphatase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00160"
FT REGION 668..703
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 789..825
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 682..698
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 806..824
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 1110
FT /note="Phosphocysteine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00160,
FT ECO:0000255|PROSITE-ProRule:PRU10044"
FT BINDING 1069
FT /ligand="substrate"
FT /evidence="ECO:0000255"
FT BINDING 1110..1116
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 1154
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT MOD_RES 577
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355"
FT MOD_RES 589
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 590
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 637
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 673
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 710
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q62728"
FT MOD_RES 711
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q62728"
FT MOD_RES 799
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 801
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 806
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
SQ SEQUENCE 1176 AA; 133491 MW; 529FBE22F1335B75 CRC64;
MPLPFGLKLK RTRRYTVSSK SCLVARIQLL NNEFVEFTLS VESTGQESLE AVAQRLELRE
VTYFSLWYYN KQNQRRWVDL EKPLKKQLDK HALEPTVYFG VLFYVPSVSQ LQQEITRYQY
YLQLKKDILE GNLPCTLEHA IQLAGLAVQA DFGDFDQYES QDFLQKFALL PVAWLQDEKV
LEEAAQKVAL LHQKYRGLTA PEAELLYMQE VERMDGYGEE SYPAKDSQGS DISIGACLDG
IFVKHKNGRP PVVFRWHDIA NMSHNKSFFA LELANKEETI QFQTEDMETA KYVWRLCVAR
HKFYRLNQCS LQTQAATLNS VRRDSSSRMS LPKPQPYAMP PPPQLHYNGH YTEPFASSQD
NIFVPNKNGF YCHSQTSLDR TQIDLSGRIR NGSVYSAHST NSLNTLQPYL QPSPMSSNPS
ITGSDVMRPD SLPSHRHSAL IPPSYRPTPD YETVMKQLNR GMVHADRHSH SLRNLNIGSS
YAYSRPDALV YSQPEIREHP HLTSPQSAHY PFNLNYSFHS QSPYPYPAER RPVVGAVSVP
ELTNVQLQAQ DYPAPNIMRT QVYRPPPPYP YPRPANSTPD LSRHLYISSS NPDLITRRVH
HSVQTFQEDS LPVAHSLQEV SEPLTAARHA HLQKRNSIEI AGLTHGFEGL RLKERTVSAS
AADVAPRTFS AGSQSSVFSD KMKQEGTEEQ EGGRYSHKKS LSDATMLIDS SEEDEDLEED
SSREQAISAV SEPRLTAAFS QELNYPCASA TPITGPLHIF EPKPHVTEPE KRAKDISPVH
LVVETHRPRR DGLLTPSMSE SDLTTSGRYR ARRDSVKKRP VSDLLSGKKS AVEGLPPLGG
MKKTRADAKK IGPLKLAALN GLSLSRLPLP DEGKEVSTRA TNDERCKVLE QRLEQGMVFT
EYERILKKRL VDGECSTARL PENAERNRFQ DVLPYDDARV ELVPTKENNT GYINASHIKV
SVSGIEWDYI ATQGPLQNTC QDFWQMVWEQ GVAIIAMVTA EEEGGREKSF RYWPRLGSRH
NTVTYGRFKI TTRFRTDSGC YATTGLKMKH LLTGQERTVW HLQYTDWPEH GCPEDLKGFL
SYLEEIQSVR RHTNSTSEPK SHNPPLLVHC SAGVGRTGVV ILSEIMVACL EHNEVLDIPR
VLDMLRQQRM MLVQTLGQYT FVYRVLIQFL KSSRLI
