PTOV1_MOUSE
ID PTOV1_MOUSE Reviewed; 416 AA.
AC Q91VU8; Q9CTC2;
DT 02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Prostate tumor-overexpressed gene 1 protein homolog;
GN Name=Ptov1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Kidney, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-53, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May activate transcription. Required for nuclear
CC translocation of FLOT1. Promotes cell proliferation.
CC {ECO:0000250|UniProtKB:Q86YD1}.
CC -!- SUBUNIT: May interact with CREBBP. Interacts with FLOT1.
CC {ECO:0000250|UniProtKB:Q86YD1}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q86YD1}. Nucleus
CC {ECO:0000250|UniProtKB:Q86YD1}. Cell membrane
CC {ECO:0000250|UniProtKB:Q86YD1}. Cytoplasm, perinuclear region
CC {ECO:0000250|UniProtKB:Q86YD1}. Note=Translocates from the cytoplasm to
CC the nucleus at the onset of S-phase. Also localizes to lipid rafts.
CC {ECO:0000250|UniProtKB:Q86YD1}.
CC -!- PTM: Ubiquitinated by the CRL2(KLHDC2) complex, which recognizes the
CC diglycine (Gly-Gly) at the C-terminus, leading to its degradation.
CC Ubiquitinated by the CRL2(APPBP2) complex, which recognizes the Arg-
CC Xaa-Xaa-Gly sequence at the C-terminus, leading to its degradation.
CC {ECO:0000250|UniProtKB:Q86YD1}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 25 family. PTOV1
CC subfamily. {ECO:0000305}.
CC -!- CAUTION: Despite sequence similarity to MED25, to date this protein has
CC not been identified as a component of the Mediator complex.
CC {ECO:0000305}.
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DR EMBL; BC008543; AAH08543.1; -; mRNA.
DR EMBL; BC024632; AAH24632.1; -; mRNA.
DR EMBL; AK003974; BAB23101.1; -; mRNA.
DR CCDS; CCDS21219.1; -.
DR RefSeq; NP_598710.1; NM_133949.1.
DR AlphaFoldDB; Q91VU8; -.
DR SMR; Q91VU8; -.
DR BioGRID; 220005; 1.
DR STRING; 10090.ENSMUSP00000035281; -.
DR iPTMnet; Q91VU8; -.
DR PhosphoSitePlus; Q91VU8; -.
DR MaxQB; Q91VU8; -.
DR PaxDb; Q91VU8; -.
DR PeptideAtlas; Q91VU8; -.
DR PRIDE; Q91VU8; -.
DR ProteomicsDB; 302011; -.
DR Antibodypedia; 18733; 133 antibodies from 24 providers.
DR Ensembl; ENSMUST00000046575; ENSMUSP00000035281; ENSMUSG00000038502.
DR GeneID; 84113; -.
DR KEGG; mmu:84113; -.
DR UCSC; uc009grk.1; mouse.
DR CTD; 53635; -.
DR MGI; MGI:1933946; Ptov1.
DR VEuPathDB; HostDB:ENSMUSG00000038502; -.
DR eggNOG; ENOG502SS25; Eukaryota.
DR GeneTree; ENSGT00940000161923; -.
DR HOGENOM; CLU_045342_0_0_1; -.
DR InParanoid; Q91VU8; -.
DR OMA; ELDCLTH; -.
DR OrthoDB; 340324at2759; -.
DR PhylomeDB; Q91VU8; -.
DR TreeFam; TF329598; -.
DR BioGRID-ORCS; 84113; 5 hits in 72 CRISPR screens.
DR ChiTaRS; Ptov1; mouse.
DR PRO; PR:Q91VU8; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q91VU8; protein.
DR Bgee; ENSMUSG00000038502; Expressed in cortical plate and 269 other tissues.
DR ExpressionAtlas; Q91VU8; baseline and differential.
DR Genevisible; Q91VU8; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 2.40.290.30; -; 2.
DR InterPro; IPR045105; Med25/PTOV1.
DR InterPro; IPR021394; Med25_PTOV.
DR InterPro; IPR038196; Med25_PTOV_sf.
DR PANTHER; PTHR12433; PTHR12433; 2.
DR Pfam; PF11232; Med25; 2.
PE 1: Evidence at protein level;
KW Activator; Cell membrane; Cytoplasm; Membrane; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation;
KW Ubl conjugation.
FT CHAIN 1..416
FT /note="Prostate tumor-overexpressed gene 1 protein homolog"
FT /id="PRO_0000304966"
FT REGION 1..54
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 184..416
FT /note="Interaction with FLOT1"
FT /evidence="ECO:0000250"
FT MOD_RES 53
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 416 AA; 46865 MW; 706671932F61141D CRC64;
MVRPRRAPHR SGAGGPLGGR GRPPRPLVVR AVRSRSWPGG PRGPQPPRIR ARSAPPMEGA
RVFGALGPIG PSSPGLTLGG LAVNEHRLSN KLLAWSGVLE WQEKRRPFSD STAKLKRTLP
CQAYVNQGEN LETDQWPQKL IMQLIPQQLL TTLGPLFRNS QLAQFHFTNR DCDSLKGLCR
IMGNGFAGCM LFPHISPCEV RVLMLLYSSK KKIFMGLIPY DQSGFVNAIR QVITTRKQAV
GPGGVHSGPV QIVNNKFLAW SGVMEWQEPR PEPNSRSKRW LPSHVYVNQG EILRTDQWPR
RLFMQLIPQQ LLTTLVPLFR NSRLVQFHFT KDMETLKSLC RIMDNGFAGC VHFSYKASCE
VRVLMLLYSS EKKIFIGLIP HDQSNFVNGI RRVIANQQQV LQRSLEQEQQ QRGMGG
