PTPB1_ECOLI
ID PTPB1_ECOLI Reviewed; 158 AA.
AC P42909; Q2M972;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=N-acetylgalactosamine-specific phosphotransferase enzyme IIB component 1;
DE EC=2.7.1.-;
DE AltName: Full=EIIB-Aga;
DE AltName: Full=PTS system N-acetylgalactosamine-specific EIIB component 1;
GN Name=agaB; Synonyms=yraD; OrderedLocusNames=b3138, JW3107;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=C;
RX PubMed=10931310; DOI=10.1046/j.1365-2958.2000.01969.x;
RA Brinkkoetter A., Kloess H., Alpert C.-A., Lengeler J.W.;
RT "Pathways for the utilization of N-acetyl-galactosamine and galactosamine
RT in Escherichia coli.";
RL Mol. Microbiol. 37:125-135(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP DISCUSSION OF SEQUENCE.
RX PubMed=8932697; DOI=10.1099/13500872-142-2-231;
RA Reizer J., Ramseier T.M., Reizer A., Charbit A., Saier M.H. Jr.;
RT "Novel phosphotransferase genes revealed by bacterial genome sequencing: a
RT gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in
RT Escherichia coli.";
RL Microbiology 142:231-250(1996).
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC system (sugar PTS), a major carbohydrate active -transport system,
CC catalyzes the phosphorylation of incoming sugar substrates
CC concomitantly with their translocation across the cell membrane. This
CC system is involved in N-acetylgalactosamine transport.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- DOMAIN: The EIIB domain is phosphorylated by phospho-EIIA on a
CC cysteinyl or histidyl residue, depending on the transported sugar.
CC Then, it transfers the phosphoryl group to the sugar substrate
CC concomitantly with the sugar uptake processed by the EIIC domain.
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DR EMBL; AF228498; AAF81090.1; -; Genomic_DNA.
DR EMBL; U18997; AAA57941.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76172.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77184.1; -; Genomic_DNA.
DR PIR; F65103; F65103.
DR RefSeq; NP_417607.1; NC_000913.3.
DR RefSeq; WP_000203711.1; NZ_LN832404.1.
DR AlphaFoldDB; P42909; -.
DR SMR; P42909; -.
DR BioGRID; 4259399; 11.
DR IntAct; P42909; 5.
DR STRING; 511145.b3138; -.
DR TCDB; 4.A.6.1.5; the pts mannose-fructose-sorbose (man) family.
DR PaxDb; P42909; -.
DR PRIDE; P42909; -.
DR EnsemblBacteria; AAC76172; AAC76172; b3138.
DR EnsemblBacteria; BAE77184; BAE77184; BAE77184.
DR GeneID; 947653; -.
DR KEGG; ecj:JW3107; -.
DR KEGG; eco:b3138; -.
DR PATRIC; fig|1411691.4.peg.3592; -.
DR EchoBASE; EB2622; -.
DR eggNOG; COG3444; Bacteria.
DR HOGENOM; CLU_116175_2_1_6; -.
DR InParanoid; P42909; -.
DR OMA; VAYGWAT; -.
DR PhylomeDB; P42909; -.
DR BioCyc; EcoCyc:AGAB-MON; -.
DR PRO; PR:P42909; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd00001; PTS_IIB_man; 1.
DR Gene3D; 3.40.35.10; -; 1.
DR InterPro; IPR004720; PTS_IIB_sorbose-sp.
DR InterPro; IPR036667; PTS_IIB_sorbose-sp_sf.
DR InterPro; IPR018455; PTS_IIB_sorbose-sp_subgr.
DR Pfam; PF03830; PTSIIB_sorb; 1.
DR SUPFAM; SSF52728; SSF52728; 1.
DR TIGRFAMs; TIGR00854; pts-sorbose; 1.
DR PROSITE; PS51101; PTS_EIIB_TYPE_4; 1.
PE 4: Predicted;
KW Cytoplasm; Kinase; Phosphotransferase system; Reference proteome;
KW Sugar transport; Transferase; Transport.
FT CHAIN 1..158
FT /note="N-acetylgalactosamine-specific phosphotransferase
FT enzyme IIB component 1"
FT /id="PRO_0000186658"
FT DOMAIN 1..158
FT /note="PTS EIIB type-4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00424"
FT ACT_SITE 17
FT /note="Pros-phosphohistidine intermediate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 158 AA; 17622 MW; 897BA2D5FED89159 CRC64;
MTSPNILLTR IDNRLVHGQV GVTWTSTIGA NLLVVVDDVV ANDDIQQKLM GITAETYGFG
IRFFTIEKTI NVIGKAAPHQ KIFLICRTPQ TVRKLVEGGI DLKDVNVGNM HFSEGKKQIS
SKVYVDDQDL TDLRFIKQRG VNVFIQDVPG DQKEQIPD
