PTPD_ECOLI
ID PTPD_ECOLI Reviewed; 263 AA.
AC P42911; Q2M970;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=N-acetylgalactosamine permease IID component;
DE AltName: Full=EIID-Aga;
DE AltName: Full=PTS system N-acetylgalactosamine-specific EIID component;
GN Name=agaD; Synonyms=yraF; OrderedLocusNames=b3140, JW3109;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=C;
RX PubMed=10931310; DOI=10.1046/j.1365-2958.2000.01969.x;
RA Brinkkoetter A., Kloess H., Alpert C.-A., Lengeler J.W.;
RT "Pathways for the utilization of N-acetyl-galactosamine and galactosamine
RT in Escherichia coli.";
RL Mol. Microbiol. 37:125-135(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP DISCUSSION OF SEQUENCE.
RX PubMed=8932697; DOI=10.1099/13500872-142-2-231;
RA Reizer J., Ramseier T.M., Reizer A., Charbit A., Saier M.H. Jr.;
RT "Novel phosphotransferase genes revealed by bacterial genome sequencing: a
RT gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in
RT Escherichia coli.";
RL Microbiology 142:231-250(1996).
RN [5]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC system (PTS), a major carbohydrate active -transport system, catalyzes
CC the phosphorylation of incoming sugar substrates concomitant with their
CC translocation across the cell membrane. This system is involved in N-
CC acetylgalactosamine transport.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00431, ECO:0000269|PubMed:15919996}; Multi-pass membrane
CC protein {ECO:0000255|PROSITE-ProRule:PRU00431,
CC ECO:0000269|PubMed:15919996}.
CC -!- DOMAIN: The EIID domain, with its homologous EIIC domain, forms the PTS
CC system translocation channel and contains part of its specific
CC substrate-binding site.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA57943.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF228498; AAF81092.1; -; Genomic_DNA.
DR EMBL; U18997; AAA57943.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC76174.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77186.1; -; Genomic_DNA.
DR PIR; H65103; H65103.
DR RefSeq; NP_417609.1; NC_000913.3.
DR RefSeq; WP_000534347.1; NZ_LN832404.1.
DR AlphaFoldDB; P42911; -.
DR SMR; P42911; -.
DR BioGRID; 4261993; 7.
DR IntAct; P42911; 2.
DR STRING; 511145.b3140; -.
DR TCDB; 4.A.6.1.5; the pts mannose-fructose-sorbose (man) family.
DR PaxDb; P42911; -.
DR PRIDE; P42911; -.
DR EnsemblBacteria; AAC76174; AAC76174; b3140.
DR EnsemblBacteria; BAE77186; BAE77186; BAE77186.
DR GeneID; 947649; -.
DR KEGG; ecj:JW3109; -.
DR KEGG; eco:b3140; -.
DR PATRIC; fig|1411691.4.peg.3590; -.
DR EchoBASE; EB2624; -.
DR eggNOG; COG3716; Bacteria.
DR HOGENOM; CLU_060742_1_0_6; -.
DR OMA; SEQMAIV; -.
DR PhylomeDB; P42911; -.
DR BioCyc; EcoCyc:AGAD-MON; -.
DR PRO; PR:P42911; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IBA:GO_Central.
DR InterPro; IPR004704; PTS_IID_man.
DR Pfam; PF03613; EIID-AGA; 1.
DR TIGRFAMs; TIGR00828; EIID-AGA; 1.
DR PROSITE; PS51108; PTS_EIID; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Phosphotransferase system;
KW Reference proteome; Sugar transport; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..263
FT /note="N-acetylgalactosamine permease IID component"
FT /id="PRO_0000186662"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00431"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00431"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00431"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00431"
FT TRANSMEM 215..235
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00431"
FT TRANSMEM 243..263
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00431"
FT DOMAIN 3..263
FT /note="PTS EIID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00431"
SQ SEQUENCE 263 AA; 29001 MW; 11573AF3503C53F1 CRC64;
MGSEISKKDI TRLGFRSSLL QASFNYERMQ AGGFTWAMLP ILKKIYKDDK PGLSAAMKDN
LEFINTHPNL VGFLMGLLIS MEEKGENRDT IKGLKVALFG PIAGIGDAIF WFTLLPIMAG
ICSSFASQGN LLGPILFFAV YLLIFFLRVG WTHVGYSVGV KAIDKVRENS QMIARSATIL
GITVIGGLIA SYVHINVVTS FAIDNTHSVA LQQDFFDKVF PNILPMAYTL LMYYFLRVKK
AHPVLLIGVT FVLSIVCSAF GIL
