PTPS_CAEEL
ID PTPS_CAEEL Reviewed; 140 AA.
AC O02058; Q2MGF4;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 10-OCT-2003, sequence version 2.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Putative 6-pyruvoyl tetrahydrobiopterin synthase;
DE Short=PTP synthase;
DE Short=PTPS;
DE EC=4.2.3.12;
GN Name=ptps-1; ORFNames=B0041.6;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Involved in the biosynthesis of tetrahydrobiopterin, an
CC essential cofactor of aromatic amino acid hydroxylases. Catalyzes the
CC transformation of 7,8-dihydroneopterin triphosphate into 6-pyruvoyl
CC tetrahydropterin (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-
CC tetrahydropterin + H(+) + triphosphate; Xref=Rhea:RHEA:22048,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:18036, ChEBI:CHEBI:58462,
CC ChEBI:CHEBI:136564; EC=4.2.3.12;
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC -!- PATHWAY: Cofactor biosynthesis; tetrahydrobiopterin biosynthesis;
CC tetrahydrobiopterin from 7,8-dihydroneopterin triphosphate: step 1/3.
CC -!- SUBUNIT: Homohexamer formed of two homotrimers in a head to head
CC fashion. {ECO:0000250}.
CC -!- INTERACTION:
CC O02058; O02058: ptps-1; NbExp=4; IntAct=EBI-312091, EBI-312091;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a;
CC IsoId=O02058-1; Sequence=Displayed;
CC Name=b;
CC IsoId=O02058-2; Sequence=VSP_019291;
CC -!- MISCELLANEOUS: The active site is at the interface between 2 subunits.
CC The proton acceptor Cys is on one subunit, and the charge relay system
CC is on the other subunit.
CC -!- SIMILARITY: Belongs to the PTPS family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FO080106; CCD61251.1; -; Genomic_DNA.
DR EMBL; FO080106; CCD61252.1; -; Genomic_DNA.
DR PIR; T34033; T34033.
DR RefSeq; NP_001040626.1; NM_001047161.1. [O02058-1]
DR RefSeq; NP_001040627.1; NM_001047162.2. [O02058-2]
DR PDB; 2G64; X-ray; 1.80 A; A=1-140.
DR PDBsum; 2G64; -.
DR AlphaFoldDB; O02058; -.
DR SMR; O02058; -.
DR BioGRID; 46692; 3.
DR IntAct; O02058; 1.
DR STRING; 6239.B0041.6a; -.
DR EPD; O02058; -.
DR PaxDb; O02058; -.
DR PeptideAtlas; O02058; -.
DR EnsemblMetazoa; B0041.6a.1; B0041.6a.1; WBGene00015010. [O02058-1]
DR EnsemblMetazoa; B0041.6b.1; B0041.6b.1; WBGene00015010. [O02058-2]
DR GeneID; 181823; -.
DR KEGG; cel:CELE_B0041.6; -.
DR UCSC; B0041.6b; c. elegans. [O02058-1]
DR CTD; 181823; -.
DR WormBase; B0041.6a; CE34237; WBGene00015010; ptps-1. [O02058-1]
DR WormBase; B0041.6b; CE39541; WBGene00015010; ptps-1. [O02058-2]
DR eggNOG; KOG4105; Eukaryota.
DR GeneTree; ENSGT00390000002752; -.
DR InParanoid; O02058; -.
DR OMA; NVAIFIW; -.
DR OrthoDB; 1502673at2759; -.
DR PhylomeDB; O02058; -.
DR Reactome; R-CEL-1474151; Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
DR UniPathway; UPA00849; UER00819.
DR EvolutionaryTrace; O02058; -.
DR PRO; PR:O02058; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00015010; Expressed in larva and 4 other tissues.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0003874; F:6-pyruvoyltetrahydropterin synthase activity; IMP:WormBase.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006729; P:tetrahydrobiopterin biosynthetic process; IMP:WormBase.
DR Gene3D; 3.30.479.10; -; 1.
DR InterPro; IPR007115; 6-PTP_synth/QueD.
DR InterPro; IPR038418; 6-PTP_synth/QueD_sf.
DR InterPro; IPR022470; PTPS_Cys_AS.
DR PANTHER; PTHR12589; PTHR12589; 1.
DR Pfam; PF01242; PTPS; 1.
DR TIGRFAMs; TIGR00039; 6PTHBS; 1.
DR PROSITE; PS00987; PTPS_1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Lyase; Metal-binding;
KW Reference proteome; Tetrahydrobiopterin biosynthesis; Zinc.
FT CHAIN 1..140
FT /note="Putative 6-pyruvoyl tetrahydrobiopterin synthase"
FT /id="PRO_0000057919"
FT ACT_SITE 38
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10123"
FT ACT_SITE 84
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 129
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT BINDING 19
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10123"
FT BINDING 44
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10123"
FT BINDING 46
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10123"
FT VAR_SEQ 1..3
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_019291"
FT STRAND 6..19
FT /evidence="ECO:0007829|PDB:2G64"
FT HELIX 28..35
FT /evidence="ECO:0007829|PDB:2G64"
FT HELIX 36..39
FT /evidence="ECO:0007829|PDB:2G64"
FT STRAND 44..57
FT /evidence="ECO:0007829|PDB:2G64"
FT TURN 60..62
FT /evidence="ECO:0007829|PDB:2G64"
FT HELIX 68..80
FT /evidence="ECO:0007829|PDB:2G64"
FT TURN 81..84
FT /evidence="ECO:0007829|PDB:2G64"
FT HELIX 87..90
FT /evidence="ECO:0007829|PDB:2G64"
FT HELIX 92..95
FT /evidence="ECO:0007829|PDB:2G64"
FT HELIX 101..115
FT /evidence="ECO:0007829|PDB:2G64"
FT HELIX 119..121
FT /evidence="ECO:0007829|PDB:2G64"
FT STRAND 122..130
FT /evidence="ECO:0007829|PDB:2G64"
FT STRAND 133..137
FT /evidence="ECO:0007829|PDB:2G64"
SQ SEQUENCE 140 AA; 16046 MW; 7A677CE73A95882C CRC64;
MFRMPIVTME RVDSFSAAHR LHSEKLSDAE NKETFGKCNN SNGHGHNYVW KVKLRGEVDP
TSGMVYDLAK LKKEMSLVLD TVDHRNLDKD VEFFKTTVST SENVAIYMFE KLKSVMSNPS
VLYKVTIEET PKNIFTYKGC
