ID   PTQB_ECOL6              Reviewed;         106 AA.
AC   P69796; P17409;
DT   10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=PTS system N,N'-diacetylchitobiose-specific EIIB component {ECO:0000250|UniProtKB:P69795};
DE   AltName: Full=EIIB-Chb {ECO:0000250|UniProtKB:P69795};
DE   AltName: Full=IVcel {ECO:0000250|UniProtKB:P69795};
DE   AltName: Full=N,N'-diacetylchitobiose-specific phosphotransferase enzyme IIB component {ECO:0000250|UniProtKB:P69795};
DE            EC=2.7.1.196 {ECO:0000250|UniProtKB:P69795};
GN   Name=chbB; Synonyms=celA; OrderedLocusNames=c2137;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: The phosphoenolpyruvate-dependent sugar phosphotransferase
CC       system (sugar PTS), a major carbohydrate active transport system,
CC       catalyzes the phosphorylation of incoming sugar substrates
CC       concomitantly with their translocation across the cell membrane. The
CC       enzyme II ChbABC PTS system is involved in the transport of the chitin
CC       disaccharide N,N'-diacetylchitobiose (GlcNAc2).
CC       {ECO:0000250|UniProtKB:P69795}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=N(pros)-phospho-L-histidyl-[protein] + N,N'-
CC         diacetylchitobiose(out) = diacetylchitobiose-6'-phosphate(in) + L-
CC         histidyl-[protein]; Xref=Rhea:RHEA:33423, Rhea:RHEA-COMP:9745,
CC         Rhea:RHEA-COMP:9746, ChEBI:CHEBI:28681, ChEBI:CHEBI:29979,
CC         ChEBI:CHEBI:64837, ChEBI:CHEBI:64883; EC=2.7.1.196;
CC         Evidence={ECO:0000250|UniProtKB:P69795};
CC   -!- SUBUNIT: Forms a complex with ChbA (EIIA). ChbB is a monomer in both
CC       its unphosphorylated and phosphorylated forms.
CC       {ECO:0000250|UniProtKB:P69795}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- INDUCTION: By GlcNAc2, GlcNAc3 and beta-N,N'-diacetylchitobiose (Me-
CC       TCB). {ECO:0000250|UniProtKB:P69795}.
CC   -!- DOMAIN: The PTS EIIB type-3 domain is phosphorylated by phospho-EIIA on
CC       a cysteinyl residue. Then, it transfers the phosphoryl group to the
CC       sugar substrate concomitantly with the sugar uptake processed by the
CC       PTS EIIC type-3 domain. {ECO:0000255|PROSITE-ProRule:PRU00423}.
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DR   EMBL; AE014075; AAN80596.1; -; Genomic_DNA.
DR   RefSeq; WP_000412169.1; NC_004431.1.
DR   AlphaFoldDB; P69796; -.
DR   BMRB; P69796; -.
DR   SMR; P69796; -.
DR   STRING; 199310.c2137; -.
DR   EnsemblBacteria; AAN80596; AAN80596; c2137.
DR   GeneID; 67415558; -.
DR   KEGG; ecc:c2137; -.
DR   eggNOG; COG1440; Bacteria.
DR   HOGENOM; CLU_147323_2_0_6; -.
DR   OMA; DAEIFAC; -.
DR   BioCyc; ECOL199310:C2137-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008982; F:protein-N(PI)-phosphohistidine-sugar phosphotransferase activity; IEA:InterPro.
DR   GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   InterPro; IPR036095; PTS_EIIB-like_sf.
DR   InterPro; IPR003501; PTS_EIIB_2/3.
DR   InterPro; IPR013012; PTS_EIIB_3.
DR   Pfam; PF02302; PTS_IIB; 1.
DR   SUPFAM; SSF52794; SSF52794; 1.
DR   TIGRFAMs; TIGR00853; pts-lac; 1.
DR   PROSITE; PS51100; PTS_EIIB_TYPE_3; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Kinase; Phosphoprotein; Phosphotransferase system;
KW   Sugar transport; Transferase; Transport.
FT   CHAIN           1..106
FT                   /note="PTS system N,N'-diacetylchitobiose-specific EIIB
FT                   component"
FT                   /id="PRO_0000186490"
FT   DOMAIN          3..106
FT                   /note="PTS EIIB type-3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00423"
FT   ACT_SITE        10
FT                   /note="Phosphocysteine intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:P69795"
FT   MOD_RES         10
FT                   /note="Phosphocysteine; by EIIA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00423"
SQ   SEQUENCE   106 AA;  11427 MW;  DE4AADE300D18F53 CRC64;
     MEKKHIYLFC SAGMSTSLLV SKMRAQAEKY EVPVIIEAFP ETLAGEKGQN ADVVLLGPQI
     AYMLPEIQRL LPNKPVEVID SLLYGKVDGL GVLKAAVAAI KKAAAN