PTR12_ARATH
ID PTR12_ARATH Reviewed; 567 AA.
AC Q8VYE4; O04553;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Protein NRT1/ PTR FAMILY 4.5;
DE Short=AtNPF4.5;
DE AltName: Full=Protein ABA-IMPORTING TRANSPORTER 2;
GN Name=NPF4.5; Synonyms=AIT2; OrderedLocusNames=At1g27040; ORFNames=T7N9.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [5]
RP GENE FAMILY.
RX PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA Gong J.M.;
RT "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT from the xylem sap and mediates cadmium tolerance.";
RL Plant Cell 22:1633-1646(2010).
RN [6]
RP FUNCTION.
RX PubMed=22645333; DOI=10.1073/pnas.1203567109;
RA Kanno Y., Hanada A., Chiba Y., Ichikawa T., Nakazawa M., Matsui M.,
RA Koshiba T., Kamiya Y., Seo M.;
RT "Identification of an abscisic acid transporter by functional screening
RT using the receptor complex as a sensor.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:9653-9658(2012).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- FUNCTION: Involved in abscisic acid transport.
CC {ECO:0000269|PubMed:22645333}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8VYE4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VYE4-2; Sequence=VSP_039947;
CC -!- TISSUE SPECIFICITY: Expressed in flowers and siliques.
CC {ECO:0000269|PubMed:17481610}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
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DR EMBL; AC000348; AAF79877.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30771.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30772.1; -; Genomic_DNA.
DR EMBL; AY072134; AAL59956.1; -; mRNA.
DR EMBL; AY096445; AAM20085.1; -; mRNA.
DR PIR; C86397; C86397.
DR RefSeq; NP_174024.2; NM_102466.4. [Q8VYE4-1]
DR RefSeq; NP_973919.1; NM_202190.1. [Q8VYE4-2]
DR AlphaFoldDB; Q8VYE4; -.
DR SMR; Q8VYE4; -.
DR BioGRID; 24828; 1.
DR IntAct; Q8VYE4; 1.
DR STRING; 3702.AT1G27040.1; -.
DR PaxDb; Q8VYE4; -.
DR PRIDE; Q8VYE4; -.
DR ProteomicsDB; 226239; -. [Q8VYE4-1]
DR EnsemblPlants; AT1G27040.1; AT1G27040.1; AT1G27040. [Q8VYE4-1]
DR EnsemblPlants; AT1G27040.2; AT1G27040.2; AT1G27040. [Q8VYE4-2]
DR GeneID; 839593; -.
DR Gramene; AT1G27040.1; AT1G27040.1; AT1G27040. [Q8VYE4-1]
DR Gramene; AT1G27040.2; AT1G27040.2; AT1G27040. [Q8VYE4-2]
DR KEGG; ath:AT1G27040; -.
DR Araport; AT1G27040; -.
DR TAIR; locus:2205769; AT1G27040.
DR eggNOG; KOG1237; Eukaryota.
DR InParanoid; Q8VYE4; -.
DR OrthoDB; 365203at2759; -.
DR PhylomeDB; Q8VYE4; -.
DR PRO; PR:Q8VYE4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8VYE4; baseline and differential.
DR Genevisible; Q8VYE4; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR000109; POT_fam.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..567
FT /note="Protein NRT1/ PTR FAMILY 4.5"
FT /id="PRO_0000399946"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 70..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 326..346
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 411..431
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 448..468
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 491..511
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 535..555
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..4
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_039947"
SQ SEQUENCE 567 AA; 62653 MW; E5FFB226DF64DAB0 CRC64;
MEVEMHGDVS KWEGYADWRN KAALRGRHGG MLAASFVLAV EILENLAFLA NASNLVLYLK
NFMHMSLARS SSEVTTFMAT AFLLALLGGF LADAFFSTFV IFLISASIEF LGLILLTIQA
RRPSLMPPPC KSSAALRCEV VGGSKAAFLF VGLYLVSLGI GGIKGSLPSH GAEQFDEGTP
KGRKQRSTFF NYYVFCLSCG ALVAVTFVVW IEDNKGWEWG FGVSTISIFL SILVFLLGSR
FYKNKIPRGS PLTTIFKVLL AASIVSCSSK TSSNHFTSRE VQSEHEEKTP SQSLTNSLTC
LNKAIEGKTH HIWLECTVQQ VEDVKIVLKM LPIFGCTIML NCCLAQLSTY SVHQAATMNR
KIVNFNVPSA SLPVFPVVFM LILAPTYDHL IIPFARKVTK SEIGITHLQR IGVGLVLSIV
AMAVAALVEL KRKQVAREAG LLDSEETLPI TFLWIALQYL FLGSADLFTL AGLLEFFFTE
APSSMRSLAT SLSWASLALG YYLSSVMVPI VNRVTKSAGQ SPWLGEKLNR NRLDLFYWLM
CVLSVVNFLH YLFWAKRYKY ISTGSIS
