PTR13_ARATH
ID PTR13_ARATH Reviewed; 576 AA.
AC Q9LFX9; A9QUN3;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Protein NRT1/ PTR FAMILY 2.12;
DE Short=AtNPF2.12;
DE AltName: Full=Nitrate transporter 1.6;
GN Name=NPF2.12; Synonyms=NRT1:6; OrderedLocusNames=At1g27080;
GN ORFNames=T7N9.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION BY NITROGEN, SUBCELLULAR
RP LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=19050168; DOI=10.1105/tpc.107.056788;
RA Almagro A., Lin S.H., Tsay Y.F.;
RT "Characterization of the Arabidopsis nitrate transporter NRT1.6 reveals a
RT role of nitrate in early embryo development.";
RL Plant Cell 20:3289-3299(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [5]
RP GENE FAMILY.
RX PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA Gong J.M.;
RT "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT from the xylem sap and mediates cadmium tolerance.";
RL Plant Cell 22:1633-1646(2010).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- FUNCTION: Low-affinity proton-dependent nitrate transporter. Not
CC involved in dipeptides transport. Involved in delivering nitrate for
CC seed development. {ECO:0000269|PubMed:19050168}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=6 mM for nitrate at -60 mV {ECO:0000269|PubMed:19050168};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:19050168};
CC Multi-pass membrane protein {ECO:0000269|PubMed:19050168}.
CC -!- TISSUE SPECIFICITY: Expressed in flowers and siliques. Expressed in
CC vascular bundle of the siliques and in funiculus.
CC {ECO:0000269|PubMed:17481610, ECO:0000269|PubMed:19050168}.
CC -!- DEVELOPMENTAL STAGE: Expression increases after pollination.
CC {ECO:0000269|PubMed:19050168}.
CC -!- INDUCTION: Up-regulated by nitrogen starvation.
CC {ECO:0000269|PubMed:19050168}.
CC -!- DISRUPTION PHENOTYPE: Aberrant embryo development and increased seed
CC abortion. Reduced seed nitrate content. {ECO:0000269|PubMed:19050168}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79856.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; EU266070; ABX61048.1; -; mRNA.
DR EMBL; AC000348; AAF79856.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30777.1; -; Genomic_DNA.
DR PIR; E86397; E86397.
DR RefSeq; NP_174028.2; NM_102470.3.
DR AlphaFoldDB; Q9LFX9; -.
DR SMR; Q9LFX9; -.
DR STRING; 3702.AT1G27080.1; -.
DR TCDB; 2.A.17.3.14; the proton-dependent oligopeptide transporter (pot/ptr) family.
DR PaxDb; Q9LFX9; -.
DR PRIDE; Q9LFX9; -.
DR ProteomicsDB; 226439; -.
DR EnsemblPlants; AT1G27080.1; AT1G27080.1; AT1G27080.
DR GeneID; 839597; -.
DR Gramene; AT1G27080.1; AT1G27080.1; AT1G27080.
DR KEGG; ath:AT1G27080; -.
DR Araport; AT1G27080; -.
DR TAIR; locus:2205719; AT1G27080.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_2_1; -.
DR InParanoid; Q9LFX9; -.
DR OMA; RGNRIFR; -.
DR OrthoDB; 365203at2759; -.
DR PhylomeDB; Q9LFX9; -.
DR PRO; PR:Q9LFX9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LFX9; baseline and differential.
DR Genevisible; Q9LFX9; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0080054; F:low-affinity nitrate transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0043562; P:cellular response to nitrogen levels; IEP:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:TAIR.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IDA:TAIR.
DR GO; GO:0006857; P:oligopeptide transport; IEA:InterPro.
DR GO; GO:0048316; P:seed development; IMP:TAIR.
DR GO; GO:0010098; P:suspensor development; IMP:TAIR.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR000109; POT_fam.
DR InterPro; IPR018456; PTR2_symporter_CS.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS01022; PTR2_1; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Nitrate assimilation; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..576
FT /note="Protein NRT1/ PTR FAMILY 2.12"
FT /id="PRO_0000399947"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 130..150
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 364..384
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 406..426
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 441..461
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 475..495
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 522..542
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 576 AA; 64930 MW; 6FC3419448D76298 CRC64;
MGVVENRKIL PEKKLGGWRA ITFILGNETL EKLGSIGVSA NFMLYLRNVF HMEPVEAFNV
YYLWMGLTNF APLLGALISD AYIGRFKTIA YASLFSILGL MTVTLTACLP QLHPPPCNNP
HPDECDDPNK LQLGILFLGL GFLSIGSGGI RPCSIPFGVD QFDQRTEQGL KGVASFFNWY
YLTLTMVLIF SHTVVVYLQT VSWVIGFSIP TSLMACAVVL FFVGMRFYVY VKPEGSVFSG
IARVIVAARK KRDLKISLVD DGTEEYYEPP VKPGVLSKLP LTDQFKFLDK AAVILDGDLT
SEGVPANKWR LCSIQEVEEV KCLIRVVPVW SAGIISIVAM TTQATFMVFQ ATKMDRHMGP
HFEIPAASIT VISYITIGIW VPIYEHLLVP FLWRMRKFRV TLLQRMGIGI VFAILSMFTA
GFVEGVRRTR ATEMTQMSVF WLALPLILMG LCESFNFIGL IEFFNSQFPE HMRSIANSLF
PLSFAAANYL SSLLVTTVHK VSGTKDHPDW LNKDLDRGKL DYFYYLIAVL GVVNLVYFWY
CAHRYQYKAG SQIEDFNEEK SLLDIEPNQR HDQSPS
