PTR14_ARATH
ID PTR14_ARATH Reviewed; 614 AA.
AC Q9LQL2;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Protein NRT1/ PTR FAMILY 7.3;
DE Short=AtNPF7.3;
DE AltName: Full=Nitrate transporter 1.5;
GN Name=NPF7.3; Synonyms=NRT1.5; OrderedLocusNames=At1g32450;
GN ORFNames=F5D14.23;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-298.
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION, SUBCELLULAR LOCATION, DISRUPTION
RP PHENOTYPE, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=18780802; DOI=10.1105/tpc.108.060244;
RA Lin S.H., Kuo H.F., Canivenc G., Lin C.S., Lepetit M., Hsu P.K.,
RA Tillard P., Lin H.L., Wang Y.Y., Tsai C.B., Gojon A., Tsay Y.F.;
RT "Mutation of the Arabidopsis NRT1.5 nitrate transporter causes defective
RT root-to-shoot nitrate transport.";
RL Plant Cell 20:2514-2528(2008).
RN [6]
RP TISSUE SPECIFICITY, INDUCTION BY CADMIUM, DISRUPTION PHENOTYPE, AND GENE
RP FAMILY.
RX PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA Gong J.M.;
RT "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT from the xylem sap and mediates cadmium tolerance.";
RL Plant Cell 22:1633-1646(2010).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- FUNCTION: Low-affinity proton-dependent bidirectional nitrate
CC transporter. Involved in nitrate loading into xylem and not in nitrate
CC uptake. Not involved in histidine or dipeptides transport.
CC {ECO:0000269|PubMed:18780802}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=6 mM for nitrate {ECO:0000269|PubMed:18780802};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:18780802};
CC Multi-pass membrane protein {ECO:0000269|PubMed:18780802}.
CC -!- TISSUE SPECIFICITY: High expression in roots. Barely detected in
CC shoots. Expressed in root pericycle cells close to the xylem.
CC {ECO:0000269|PubMed:17481610, ECO:0000269|PubMed:18780802,
CC ECO:0000269|PubMed:20501909}.
CC -!- INDUCTION: Up-regulated slowly by nitrate. Down-regulated by cadmium
CC and high pH. Circadian-regulation. Expression increase during the dark
CC phase and decrease during the light phase.
CC {ECO:0000269|PubMed:18780802, ECO:0000269|PubMed:20501909}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype when grown under normal
CC conditions. Lower nitrate concentration in xylem sap. Decreased long-
CC distance root-to-shoot transport of nitrate but not of sulfate or
CC phosphate. {ECO:0000269|PubMed:18780802, ECO:0000269|PubMed:20501909}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF81343.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007767; AAF81343.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31488.1; -; Genomic_DNA.
DR EMBL; BX841204; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; G86449; G86449.
DR RefSeq; NP_174523.2; NM_102980.4.
DR AlphaFoldDB; Q9LQL2; -.
DR SMR; Q9LQL2; -.
DR BioGRID; 25373; 1.
DR IntAct; Q9LQL2; 1.
DR STRING; 3702.AT1G32450.1; -.
DR TCDB; 2.A.17.3.19; the proton-dependent oligopeptide transporter (pot/ptr) family.
DR PaxDb; Q9LQL2; -.
DR PRIDE; Q9LQL2; -.
DR ProteomicsDB; 224834; -.
DR EnsemblPlants; AT1G32450.1; AT1G32450.1; AT1G32450.
DR GeneID; 840139; -.
DR Gramene; AT1G32450.1; AT1G32450.1; AT1G32450.
DR KEGG; ath:AT1G32450; -.
DR Araport; AT1G32450; -.
DR TAIR; locus:2033776; AT1G32450.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_1_1; -.
DR InParanoid; Q9LQL2; -.
DR OMA; MKKHYKL; -.
DR OrthoDB; 365203at2759; -.
DR PhylomeDB; Q9LQL2; -.
DR PRO; PR:Q9LQL2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LQL2; baseline and differential.
DR Genevisible; Q9LQL2; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015386; F:potassium:proton antiporter activity; IDA:TAIR.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0010150; P:leaf senescence; IMP:TAIR.
DR GO; GO:0015706; P:nitrate transmembrane transport; IMP:TAIR.
DR GO; GO:0055075; P:potassium ion homeostasis; IDA:TAIR.
DR GO; GO:1902600; P:proton transmembrane transport; IDA:TAIR.
DR GO; GO:0010167; P:response to nitrate; IEP:TAIR.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR000109; POT_fam.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Nitrate assimilation; Phosphoprotein;
KW Reference proteome; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..614
FT /note="Protein NRT1/ PTR FAMILY 7.3"
FT /id="PRO_0000399948"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 226..246
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 355..375
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 390..410
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 435..455
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 515..535
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 559..579
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 592..614
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 111
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q05085"
SQ SEQUENCE 614 AA; 68699 MW; 19D9C8FB1E975DFD CRC64;
MSCLEIYNKD TMKKKEGEEE TRDGTVDYYG RPSIRSNSGQ WVAGIVILLN QGLATLAFFG
VGVNLVLFLT RVLQQNNADA ANNVSKWTGT VYIFSLVGAF LSDSYWGRYK TCAIFQVIFV
IGLSSLSLSS YMFLIRPRGC GDEVTPCGSH SMMEITMFYF SIYLIALGYG GYQPNIATLG
ADQFDEEHPK EGYSKIAFFS YFYLALNLGS LFSNTILGYF EDEGMWALGF WASTGSAIIG
LILFLVGTPR YRYFKPTGNP LSRFCQVLVA ATKKSSVEAP LRGREEMYDG DSEGKNASVN
TGRRIVHTDE FKFLDKAAYI TARDLDDKKQ DSVNPWRLCP VTQVEEVKCI LRLMPIWLCT
IIYSVVFTQM ASLFVEQGAA MNTSVSDFKI PPASMSSFDI LSVALFIFLY RRVLEPVANR
FKKNGSKGIT ELHRMGIGLV IAVIAMIAAG IVECYRLKYA DKSCTHCDGS SSLSIFWQAP
QYSLIGASEV FMYVGQLEFF NAQTPDGLKS FGSALCMMSM SMGNFVSSLL VTMVVKISTE
DHMPGWIPRN LNKGHLDRFY FLLAALTSID LVVYIACAKW YKPIQLEGKD EMQDMSDDDY
DTESEEEREK DSKV
