PTR21_ARATH
ID PTR21_ARATH Reviewed; 620 AA.
AC Q8RX77; Q9CAS0;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Protein NRT1/ PTR FAMILY 2.13;
DE Short=AtNPF2.13;
DE AltName: Full=Nitrate transporter 1.7;
GN Name=NPF2.13; Synonyms=NRT1.7; OrderedLocusNames=At1g69870;
GN ORFNames=T17F3.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=15308754; DOI=10.1105/tpc.104.023150;
RA Nuehse T.S., Stensballe A., Jensen O.N., Peck S.C.;
RT "Phosphoproteomics of the Arabidopsis plasma membrane and a new
RT phosphorylation site database.";
RL Plant Cell 16:2394-2405(2004).
RN [5]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [6]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, SUBCELLULAR
RP LOCATION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19734434; DOI=10.1105/tpc.109.067603;
RA Fan S.C., Lin C.S., Hsu P.K., Lin S.H., Tsay Y.F.;
RT "The Arabidopsis nitrate transporter NRT1.7, expressed in phloem, is
RT responsible for source-to-sink remobilization of nitrate.";
RL Plant Cell 21:2750-2761(2009).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [8]
RP GENE FAMILY.
RX PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA Gong J.M.;
RT "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT from the xylem sap and mediates cadmium tolerance.";
RL Plant Cell 22:1633-1646(2010).
RN [9]
RP FUNCTION.
RX PubMed=22864417; DOI=10.1038/nature11285;
RA Nour-Eldin H.H., Andersen T.G., Burow M., Madsen S.R., Jorgensen M.E.,
RA Olsen C.E., Dreyer I., Hedrich R., Geiger D., Halkier B.A.;
RT "NRT/PTR transporters are essential for translocation of glucosinolate
RT defence compounds to seeds.";
RL Nature 488:531-534(2012).
RN [10]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- FUNCTION: Low-affinity proton-dependent nitrate transporter. Not
CC involved in dipeptides transport, but has a weak glucosinolate
CC transport activity. Involved in phloem loading and nitrate
CC remobilization from the older leaves to other tissues.
CC {ECO:0000269|PubMed:19734434, ECO:0000269|PubMed:22864417}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=2.8 mM for nitrate at -60 mV {ECO:0000269|PubMed:19734434};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:19734434};
CC Multi-pass membrane protein {ECO:0000269|PubMed:19734434}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves and flowers. Detected in stems
CC and siliques. Highest expression in the distal lamina of older leaves.
CC Restricted to the sieve element and companion cell complex of the minor
CC vein. {ECO:0000269|PubMed:17481610, ECO:0000269|PubMed:19734434}.
CC -!- INDUCTION: Circadian-regulation. Expression increase during the light
CC phase and decrease during the dark phase.
CC {ECO:0000269|PubMed:19734434}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype when grown under normal
CC conditions. Growth retardation when starved of nitrogen.
CC {ECO:0000269|PubMed:19734434}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG52567.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC010675; AAG52567.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE34993.1; -; Genomic_DNA.
DR EMBL; AY090257; AAL90918.1; -; mRNA.
DR EMBL; AY125531; AAM78041.1; -; mRNA.
DR PIR; A96721; A96721.
DR RefSeq; NP_564979.1; NM_105655.3.
DR AlphaFoldDB; Q8RX77; -.
DR SMR; Q8RX77; -.
DR BioGRID; 28544; 24.
DR IntAct; Q8RX77; 24.
DR STRING; 3702.AT1G69870.1; -.
DR TCDB; 2.A.17.3.15; the proton-dependent oligopeptide transporter (pot/ptr) family.
DR iPTMnet; Q8RX77; -.
DR PaxDb; Q8RX77; -.
DR PRIDE; Q8RX77; -.
DR ProteomicsDB; 226102; -.
DR EnsemblPlants; AT1G69870.1; AT1G69870.1; AT1G69870.
DR GeneID; 843323; -.
DR Gramene; AT1G69870.1; AT1G69870.1; AT1G69870.
DR KEGG; ath:AT1G69870; -.
DR Araport; AT1G69870; -.
DR TAIR; locus:2196800; AT1G69870.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_2_1; -.
DR InParanoid; Q8RX77; -.
DR OMA; ACIPAGY; -.
DR OrthoDB; 365203at2759; -.
DR PhylomeDB; Q8RX77; -.
DR PRO; PR:Q8RX77; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8RX77; baseline and differential.
DR Genevisible; Q8RX77; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0080054; F:low-affinity nitrate transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0015706; P:nitrate transmembrane transport; IDA:TAIR.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR000109; POT_fam.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Nitrate assimilation; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..620
FT /note="Protein NRT1/ PTR FAMILY 2.13"
FT /id="PRO_0000399955"
FT TRANSMEM 70..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 364..384
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 402..422
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 443..463
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 485..505
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 524..544
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 568..588
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 620 AA; 68417 MW; 3B554DEDC4C1E829 CRC64;
MVLEDRKDGS SLPGRSGSFS KSSPSELDVV DPYKRISSPG SILDAEKVEK KPGGWRAVSF
ILGNETLERL GSIGLLANFM VYLTKVFHLE QVDAANVINI WSGFTNLTPL VGAYISDTYV
GRFKTIAFAS FATLLGLITI TLTASFPQLH PASCNSQDPL SCGGPNKLQI GVLLLGLCFL
SVGSGGIRPC SIPFGVDQFD QRTEEGVKGV ASFFNWYYMT FTVVLIITQT VVVYIQDQVS
WIIGFSIPTG LMALAVVMFF AGMKRYVYVK PEGSIFSGIA QVIVAARKKR KLKLPAEDDG
TVTYYDPAIK SSVLSKLHRS NQFRCLDKAA VVIEGDLTPE GPPADKWRLC SVQEVEEVKC
LIRIVPIWSA GIISLAAMTT QGTFTVSQAL KMDRNLGPKF EIPAGSLSVI SLLTIGIFLP
FYDRVFVPFM RRITGHKSGI TLLQRIGTGI VFAIFSMIVA GIVERMRRIR SINAGDPTGM
TPMSVFWLSP QLILMGLCEA FNIIGQIEFF NSQFPEHMRS IANSLFSLSF AGSSYLSSFL
VTVVHKFSGG HDRPDWLNKN LNAGKLDYFY YLIAVLGVVN LVYFWYCARG YRYKVGLPIE
DFEEDKSSDD VEMTSKKSMK
