PTR24_ARATH
ID PTR24_ARATH Reviewed; 538 AA.
AC Q8RX67; Q3E7P6; Q8GYF0; Q9C7H6; Q9C7U2;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Protein NRT1/ PTR FAMILY 5.11;
DE Short=AtNPF5.11;
GN Name=NPF5.11; OrderedLocusNames=At1g72130; ORFNames=F28P5.1, T9N14.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [6]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [7]
RP GENE FAMILY.
RX PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA Gong J.M.;
RT "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT from the xylem sap and mediates cadmium tolerance.";
RL Plant Cell 22:1633-1646(2010).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8RX67-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8RX67-2; Sequence=VSP_039949;
CC Name=3;
CC IsoId=Q8RX67-3; Sequence=VSP_039948;
CC -!- TISSUE SPECIFICITY: Expressed in shoots and roots.
CC {ECO:0000269|PubMed:17481610}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51131.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAG51788.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC067754; AAG51788.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC069273; AAG51131.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35278.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35279.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58805.1; -; Genomic_DNA.
DR EMBL; AK117678; BAC42330.1; -; mRNA.
DR EMBL; AY090354; AAL91259.1; -; mRNA.
DR EMBL; BT000836; AAN33211.1; -; mRNA.
DR EMBL; BX814549; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; F96744; F96744.
DR RefSeq; NP_001321216.1; NM_001334516.1. [Q8RX67-3]
DR RefSeq; NP_177358.2; NM_105871.4. [Q8RX67-1]
DR RefSeq; NP_974129.1; NM_202400.2. [Q8RX67-3]
DR AlphaFoldDB; Q8RX67; -.
DR SMR; Q8RX67; -.
DR STRING; 3702.AT1G72130.1; -.
DR PaxDb; Q8RX67; -.
DR PRIDE; Q8RX67; -.
DR ProteomicsDB; 226103; -. [Q8RX67-1]
DR EnsemblPlants; AT1G72130.1; AT1G72130.1; AT1G72130. [Q8RX67-1]
DR EnsemblPlants; AT1G72130.2; AT1G72130.2; AT1G72130. [Q8RX67-3]
DR EnsemblPlants; AT1G72130.3; AT1G72130.3; AT1G72130. [Q8RX67-3]
DR GeneID; 843544; -.
DR Gramene; AT1G72130.1; AT1G72130.1; AT1G72130. [Q8RX67-1]
DR Gramene; AT1G72130.2; AT1G72130.2; AT1G72130. [Q8RX67-3]
DR Gramene; AT1G72130.3; AT1G72130.3; AT1G72130. [Q8RX67-3]
DR KEGG; ath:AT1G72130; -.
DR Araport; AT1G72130; -.
DR TAIR; locus:2030316; AT1G72130.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_1_1; -.
DR InParanoid; Q8RX67; -.
DR OMA; IWAFVAD; -.
DR PhylomeDB; Q8RX67; -.
DR PRO; PR:Q8RX67; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8RX67; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IDA:TAIR.
DR GO; GO:0071916; F:dipeptide transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0080054; F:low-affinity nitrate transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0042937; F:tripeptide transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015706; P:nitrate transmembrane transport; IDA:TAIR.
DR CDD; cd17417; MFS_NPF5; 1.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044739; NRT1/PTR.
DR InterPro; IPR000109; POT_fam.
DR InterPro; IPR018456; PTR2_symporter_CS.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS01022; PTR2_1; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..538
FT /note="Protein NRT1/ PTR FAMILY 5.11"
FT /id="PRO_0000399958"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 389..409
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 424..444
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 463..483
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 507..527
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 99
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q05085"
FT VAR_SEQ 1..118
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14993207"
FT /id="VSP_039948"
FT VAR_SEQ 1..39
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11910074"
FT /id="VSP_039949"
FT CONFLICT 368
FT /note="L -> S (in Ref. 3; BAC42330)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 538 AA; 60047 MW; C02F764B836503C7 CRC64;
MAITYSSADE LGNTTEGFLE IRENTSGGWK SARLIIVVQM AERFAYFGIA SNLIMYLTGP
LGESTAAAAA NVNAWTGTVA FLPLLGGFLA DSYLGRFRTI IISSSLYILG LGLLSFSTMI
PSHQSKDSNQ LQETIFFFSL YLVAIGQGGY NPCIKVFGAD QFDGNDHKEA RDKSSFFNWL
MFGNCISILT TRLVSTYIQE NLSWSLGFGI PSVSMLLSLF LFLLGTTSYR FSTERVGKKN
PFARISRVFM EALKNRRQPD LDIANANANE TLLLLAHQSS KQFRFLDRAA ISCELAEIEE
AKAVLRLIPI WITSVVYTIV HAQSPTFFTK QGATMDRSIS PGLLVPAATL QSFINLSVVV
FIPIYDRLLV PFARSFTQNS SGITTLQRIG TGIFLSILAM VLAALVETKR LQAARDELSI
PMSVWWLIPQ YVIFGVSDMF TMVGLQEFFY GQVPSELRSV GMALNLSIYG AGNYLSSFMI
SVIDKITNQY GQRSWFDNDL DQAHLDYFYW LLACLGFIGF AFYLWFAKSY VYSRSNTF
