PTR25_ARATH
ID PTR25_ARATH Reviewed; 555 AA.
AC Q9C7U1; Q0WL93; Q681S0;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Protein NRT1/ PTR FAMILY 5.12;
DE Short=AtNPF5.12;
GN Name=NPF5.12; OrderedLocusNames=At1g72140; ORFNames=T9N14.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INDUCTION BY NEMATODES.
RX PubMed=16478044; DOI=10.1094/mpmi-18-1247;
RA Hammes U.Z., Schachtman D.P., Berg R.H., Nielsen E., Koch W.,
RA McIntyre L.M., Taylor C.G.;
RT "Nematode-induced changes of transporter gene expression in Arabidopsis
RT roots.";
RL Mol. Plant Microbe Interact. 18:1247-1257(2005).
RN [6]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [7]
RP GENE FAMILY.
RX PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA Gong J.M.;
RT "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT from the xylem sap and mediates cadmium tolerance.";
RL Plant Cell 22:1633-1646(2010).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in shoots and roots.
CC {ECO:0000269|PubMed:17481610}.
CC -!- INDUCTION: Up-regulated upon nematode infection.
CC {ECO:0000269|PubMed:16478044}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF02114.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR EMBL; AC067754; AAG51791.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35280.1; -; Genomic_DNA.
DR EMBL; BT015165; AAT85761.1; -; mRNA.
DR EMBL; AK175547; BAD43310.1; -; mRNA.
DR EMBL; AK230313; BAF02114.1; ALT_SEQ; mRNA.
DR PIR; G96744; G96744.
DR RefSeq; NP_177359.1; NM_105872.5.
DR AlphaFoldDB; Q9C7U1; -.
DR SMR; Q9C7U1; -.
DR STRING; 3702.AT1G72140.1; -.
DR PaxDb; Q9C7U1; -.
DR PRIDE; Q9C7U1; -.
DR ProteomicsDB; 224841; -.
DR EnsemblPlants; AT1G72140.1; AT1G72140.1; AT1G72140.
DR GeneID; 843545; -.
DR Gramene; AT1G72140.1; AT1G72140.1; AT1G72140.
DR KEGG; ath:AT1G72140; -.
DR Araport; AT1G72140; -.
DR TAIR; locus:2206991; AT1G72140.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_1_1; -.
DR InParanoid; Q9C7U1; -.
DR OMA; SLCKDQE; -.
DR OrthoDB; 365203at2759; -.
DR PhylomeDB; Q9C7U1; -.
DR PRO; PR:Q9C7U1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C7U1; baseline and differential.
DR Genevisible; Q9C7U1; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0009705; C:plant-type vacuole membrane; IDA:TAIR.
DR GO; GO:0071916; F:dipeptide transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0080054; F:low-affinity nitrate transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0042937; F:tripeptide transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015706; P:nitrate transmembrane transport; IDA:TAIR.
DR GO; GO:0009624; P:response to nematode; HEP:TAIR.
DR CDD; cd17417; MFS_NPF5; 1.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044739; NRT1/PTR.
DR InterPro; IPR000109; POT_fam.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 2: Evidence at transcript level;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..555
FT /note="Protein NRT1/ PTR FAMILY 5.12"
FT /id="PRO_0000399959"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 315..335
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 401..421
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 443..463
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 482..502
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 526..546
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 108
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q05085"
FT CONFLICT 142
FT /note="C -> Y (in Ref. 4; BAD43310)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 555 AA; 61386 MW; 7453C1FD8A7B86F6 CRC64;
MSTSIGDNET GAIVSNENVE FSVDFRGNPS IRSSSGAWKS SGFTMCAEVA EKFAYFGIAS
NLITYFTEAL GESTAVAASN VNLWLGTAAF LPLIWGSIAD SFLGRFRTIL LTSSFYIMGL
GLLTFSATIP SLCNDQETRE SCVSQVKVII FFCALYLIAL GEGGFKVCLR AFGADQFDEQ
DPNESKAKSS YFNWLYFAIS IGILTTRLVT NYVQENLSWA LGYAIPCLSM MLALFLFLLG
IKTYRFSTGG EGRQGKKHDN PFVRIGRVFV AAARNRRQTP SDTCLLLPNE STKKFRFLDR
AVISCDSYEV EEAKAVLSLI PIWLCSLVFG IVFAQSPTFF TKQGSTMDRS ISSTLQVPAA
TLQCFISLAI LVFIPIYDRL FVPIARSITR KPAGITTLQR ISTGIFLSII SMVIAALVEM
KRLKTARDHG LVDSPKATVP MSVCWLIPQY ILFGVSDVFT MVGLQEFFYG EVPPQLRSMG
LALYLSIIGI GNFLSSFMVS VIEEATSQSG QVSWFSNNLN QAHLDYFYWL LACLSSLAFI
FTVYFAKSYL YNSPK
