PTR27_ARATH
ID PTR27_ARATH Reviewed; 577 AA.
AC Q9SZY4; O48784; Q8LPH1;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Protein NRT1/ PTR FAMILY 6.2;
DE Short=AtNPF6.2;
DE AltName: Full=Nitrate transporter 1.4;
GN Name=NPF6.2; Synonyms=NRT1.4, NTP2; OrderedLocusNames=At2g26690;
GN ORFNames=F18A8.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Hatzfeld Y., Saito K.;
RT "Identification of two putative nitrate transporters highly homologous to
RT CHL 1 from Arabidopsis thaliana.";
RL (er) Plant Gene Register PGR99-018(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP INDUCTION BY NITRATE.
RX PubMed=12668777; DOI=10.1093/pcp/pcg036;
RA Okamoto M., Vidmar J.J., Glass A.D.;
RT "Regulation of NRT1 and NRT2 gene families of Arabidopsis thaliana:
RT responses to nitrate provision.";
RL Plant Cell Physiol. 44:304-317(2003).
RN [6]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=15509836; DOI=10.1093/pcp/pch143;
RA Chiu C.C., Lin C.S., Hsia A.P., Su R.C., Lin H.L., Tsay Y.F.;
RT "Mutation of a nitrate transporter, AtNRT1:4, results in a reduced petiole
RT nitrate content and altered leaf development.";
RL Plant Cell Physiol. 45:1139-1148(2004).
RN [7]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [8]
RP GENE FAMILY.
RX PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA Gong J.M.;
RT "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT from the xylem sap and mediates cadmium tolerance.";
RL Plant Cell 22:1633-1646(2010).
RN [9]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- FUNCTION: Low-affinity proton-dependent nitrate transporter. Not
CC involved in dipeptides transport. {ECO:0000269|PubMed:15509836}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=2.4 mM for nitrate at -160 mV {ECO:0000269|PubMed:15509836};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in shoots, leaves, flowers and siliques.
CC Expressed in leaf petiole. {ECO:0000269|PubMed:15509836,
CC ECO:0000269|PubMed:17481610}.
CC -!- INDUCTION: Up-regulated in the shoots by nitrate, but no changes in the
CC roots. {ECO:0000269|PubMed:12668777}.
CC -!- DISRUPTION PHENOTYPE: Wider leaves resulting from increased cell
CC expansion. Lower nitrate content of the petiole and midrib.
CC {ECO:0000269|PubMed:15509836}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB95302.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AJ011604; CAB38705.1; -; mRNA.
DR EMBL; AC003105; AAB95302.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC07875.1; -; Genomic_DNA.
DR EMBL; AY099800; AAM20651.1; -; mRNA.
DR PIR; F84663; F84663.
DR PIR; T52608; T52608.
DR RefSeq; NP_850084.1; NM_179753.1.
DR AlphaFoldDB; Q9SZY4; -.
DR SMR; Q9SZY4; -.
DR STRING; 3702.AT2G26690.1; -.
DR TCDB; 2.A.17.3.18; the proton-dependent oligopeptide transporter (pot/ptr) family.
DR PaxDb; Q9SZY4; -.
DR PRIDE; Q9SZY4; -.
DR ProteomicsDB; 224842; -.
DR EnsemblPlants; AT2G26690.1; AT2G26690.1; AT2G26690.
DR GeneID; 817210; -.
DR Gramene; AT2G26690.1; AT2G26690.1; AT2G26690.
DR KEGG; ath:AT2G26690; -.
DR Araport; AT2G26690; -.
DR TAIR; locus:2043803; AT2G26690.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_1_1; -.
DR InParanoid; Q9SZY4; -.
DR OrthoDB; 365203at2759; -.
DR PhylomeDB; Q9SZY4; -.
DR SABIO-RK; Q9SZY4; -.
DR PRO; PR:Q9SZY4; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SZY4; baseline and differential.
DR Genevisible; Q9SZY4; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0006857; P:oligopeptide transport; IEA:InterPro.
DR GO; GO:0009753; P:response to jasmonic acid; IEP:TAIR.
DR GO; GO:0009611; P:response to wounding; IEP:TAIR.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR000109; POT_fam.
DR InterPro; IPR018456; PTR2_symporter_CS.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS01022; PTR2_1; 1.
PE 1: Evidence at protein level;
KW Membrane; Nitrate assimilation; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..577
FT /note="Protein NRT1/ PTR FAMILY 6.2"
FT /id="PRO_0000399961"
FT TRANSMEM 28..48
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 214..234
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 369..389
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 447..467
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 488..508
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 535..555
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 435
FT /note="S -> C (in Ref. 4; AAM20651)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 577 AA; 63572 MW; 05409F965933D0C4 CRC64;
MESKGSWTVA DAVDYKGRPA DKSKTGGWIT AALILGIEVV ERLSTMGIAV NLVTYLMETM
HLPSSTSANI VTDFMGTSFL LCLLGGFLAD SFLGRFKTIG IFSTIQALGT GALAVATKLP
ELRPPTCHHG EACIPATAFQ MTILYVSLYL IALGTGGLKS SISGFGSDQF DDKDPKEKAH
MAFFFNRFFF FISMGTLLAV TVLVYMQDEV GRSWAYGICT VSMAIAIVIF LCGTKRYRYK
KSQGSPVVQI FQVIAAAFRK RKMELPQSIV YLYEDNPEGI RIEHTDQFHL LDKAAIVAEG
DFEQTLDGVA IPNPWKLSSV TKVEEVKMMV RLLPIWATTI IFWTTYAQMI TFSVEQASTM
RRNIGSFKIP AGSLTVFFVA AILITLAVYD RAIMPFWKKW KGKPGFSSLQ RIAIGLVLST
AGMAAAALVE QKRLSVAKSS SQKTLPISVF LLVPQFFLVG AGEAFIYTGQ LDFFITQSPK
GMKTMSTGLF LTTLSLGFFV SSFLVSIVKR VTSTSTDVGW LADNINHGRL DYFYWLLVIL
SGINFVVYII CALWFKPTKG KDSVEKENGK GFSVEDC
