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PTR2_YEAST
ID   PTR2_YEAST              Reviewed;         601 AA.
AC   P32901; D6VXF3;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 2.
DT   03-AUG-2022, entry version 166.
DE   RecName: Full=Peptide transporter PTR2;
DE   AltName: Full=Peptide permease PTR2;
GN   Name=PTR2; OrderedLocusNames=YKR093W; ORFNames=YKR413;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8264579; DOI=10.1128/mcb.14.1.104-115.1994;
RA   Perry J.R., Basrai M.A., Steiner H.-Y., Naider F., Becker J.M.;
RT   "Isolation and characterization of a Saccharomyces cerevisiae peptide
RT   transport gene.";
RL   Mol. Cell. Biol. 14:104-115(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8154186; DOI=10.1002/yea.320091209;
RA   Bou G., Esteban P.F., Baladron V., Gonzalez G.A., Cantalejo J.G.,
RA   Remacha M.A., Jimenez A., del Rey F., Ballesta J.P.G., Revuelta J.L.;
RT   "The complete sequence of a 15,820 bp segment of Saccharomyces cerevisiae
RT   chromosome XI contains the UBI2 and MPL1 genes and three new open reading
RT   frames.";
RL   Yeast 9:1349-1354(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8196765; DOI=10.1038/369371a0;
RA   Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA   Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA   Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA   Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA   Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA   Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA   Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA   Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA   Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA   Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA   Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA   Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA   Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA   Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA   Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA   Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA   Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA   Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA   Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA   van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA   von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA   Becker I., Mewes H.-W.;
RT   "Complete DNA sequence of yeast chromosome XI.";
RL   Nature 369:371-378(1994).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-37; SER-39; SER-45 AND
RP   SER-594, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ADR376;
RX   PubMed=17330950; DOI=10.1021/pr060559j;
RA   Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA   Elias J.E., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of alpha-factor-arrested
RT   Saccharomyces cerevisiae.";
RL   J. Proteome Res. 6:1190-1197(2007).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39 AND SER-594, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- FUNCTION: Uptake of small peptides.
CC   -!- INTERACTION:
CC       P32901; Q04182: PDR15; NbExp=2; IntAct=EBI-20799497, EBI-13072;
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC       dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC       {ECO:0000305}.
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DR   EMBL; L11994; AAC37368.1; -; Unassigned_DNA.
DR   EMBL; X73541; CAA51947.1; -; Genomic_DNA.
DR   EMBL; Z28318; CAA82172.1; -; Genomic_DNA.
DR   EMBL; BK006944; DAA09243.1; -; Genomic_DNA.
DR   PIR; S38171; S38171.
DR   RefSeq; NP_013019.1; NM_001179883.1.
DR   AlphaFoldDB; P32901; -.
DR   SMR; P32901; -.
DR   BioGRID; 34224; 122.
DR   DIP; DIP-5311N; -.
DR   IntAct; P32901; 3.
DR   MINT; P32901; -.
DR   STRING; 4932.YKR093W; -.
DR   TCDB; 2.A.17.2.2; the proton-dependent oligopeptide transporter (pot/ptr) family.
DR   CarbonylDB; P32901; -.
DR   iPTMnet; P32901; -.
DR   MaxQB; P32901; -.
DR   PaxDb; P32901; -.
DR   PRIDE; P32901; -.
DR   DNASU; 853968; -.
DR   EnsemblFungi; YKR093W_mRNA; YKR093W; YKR093W.
DR   GeneID; 853968; -.
DR   KEGG; sce:YKR093W; -.
DR   SGD; S000001801; PTR2.
DR   VEuPathDB; FungiDB:YKR093W; -.
DR   eggNOG; KOG1237; Eukaryota.
DR   HOGENOM; CLU_004790_4_2_1; -.
DR   InParanoid; P32901; -.
DR   OMA; QMMGVWF; -.
DR   BioCyc; YEAST:G3O-32056-MON; -.
DR   Reactome; R-SCE-427975; Proton/oligopeptide cotransporters.
DR   Reactome; R-SCE-6798695; Neutrophil degranulation.
DR   PRO; PR:P32901; -.
DR   Proteomes; UP000002311; Chromosome XI.
DR   RNAct; P32901; protein.
DR   GO; GO:0071944; C:cell periphery; HDA:SGD.
DR   GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR   GO; GO:0071916; F:dipeptide transmembrane transporter activity; IDA:SGD.
DR   GO; GO:1904680; F:peptide transmembrane transporter activity; IMP:SGD.
DR   GO; GO:0042937; F:tripeptide transmembrane transporter activity; IDA:SGD.
DR   GO; GO:0042938; P:dipeptide transport; IDA:SGD.
DR   GO; GO:0015833; P:peptide transport; IMP:SGD.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0042939; P:tripeptide transport; IDA:SGD.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR000109; POT_fam.
DR   InterPro; IPR018456; PTR2_symporter_CS.
DR   PANTHER; PTHR11654; PTHR11654; 1.
DR   Pfam; PF00854; PTR2; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS01022; PTR2_1; 1.
DR   PROSITE; PS01023; PTR2_2; 1.
PE   1: Evidence at protein level;
KW   Membrane; Peptide transport; Phosphoprotein; Protein transport;
KW   Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..601
FT                   /note="Peptide transporter PTR2"
FT                   /id="PRO_0000064317"
FT   TOPO_DOM        1..150
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        151..172
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        173..182
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        183..202
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        203..210
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        211..229
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        230..267
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        268..287
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        288..294
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        295..316
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        317..378
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        379..399
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        400..412
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        413..429
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        430..448
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        449..466
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        467..494
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        495..513
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        514..526
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        527..547
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        548..554
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        555..577
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        578..601
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          1..66
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        9..31
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        32..51
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         37
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0007744|PubMed:17330950"
FT   MOD_RES         39
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17330950,
FT                   ECO:0007744|PubMed:19779198"
FT   MOD_RES         45
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17330950"
FT   MOD_RES         594
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17330950,
FT                   ECO:0007744|PubMed:19779198"
FT   CONFLICT        39
FT                   /note="S -> T (in Ref. 1; AAC37368)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   601 AA;  68044 MW;  2BD50723B1A3B310 CRC64;
     MLNHPSQGSD DAQDEKQGDF PVIEEEKTQA VTLKDSYVSD DVANSTERYN LSPSPEDEDF
     EGPTEEEMQT LRHVGGKIPM RCWLIAIVEL SERFSYYGLS APFQNYMEYG PNDSPKGVLS
     LNSQGATGLS YFFQFWCYVT PVFGGYVADT FWGKYNTICC GTAIYIAGIF ILFITSIPSV
     GNRDSAIGGF IAAIILIGIA TGMIKANLSV LIADQLPKRK PSIKVLKSGE RVIVDSNITL
     QNVFMFFYFM INVGSLSLMA TTELEYHKGF WAAYLLPFCF FWIAVVTLIF GKKQYIQRPI
     GDKVIAKSFK VCWILTKNKF DFNAAKPSVH PEKNYPWNDK FVDEIKRALA ACKVFIFYPI
     YWTQYGTMIS SFITQASMME LHGIPNDFLQ AFDSIALIIF IPIFEKFVYP FIRRYTPLKP
     ITKIFFGFMF GSFAMTWAAV LQSFVYKAGP WYNEPLGHNT PNHVHVCWQI PAYVLISFSE
     IFASITGLEY AYSKAPASMK SFIMSIFLLT NAFGSAIGCA LSPVTVDPKF TWLFTGLAVA
     CFISGCLFWL CFRKYNDTEE EMNAMDYEEE DEFDLNPISA PKANDIEILE PMESLRSTTK
     Y
 
 
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