PTR30_ARATH
ID PTR30_ARATH Reviewed; 583 AA.
AC Q8VZR7; O22889;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Protein NRT1/ PTR FAMILY 5.1;
DE Short=AtNPF5.1;
GN Name=NPF5.1; OrderedLocusNames=At2g40460; ORFNames=T2P4.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP INDUCTION BY NEMATODES.
RX PubMed=16478044; DOI=10.1094/mpmi-18-1247;
RA Hammes U.Z., Schachtman D.P., Berg R.H., Nielsen E., Koch W.,
RA McIntyre L.M., Taylor C.G.;
RT "Nematode-induced changes of transporter gene expression in Arabidopsis
RT roots.";
RL Mol. Plant Microbe Interact. 18:1247-1257(2005).
RN [5]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [6]
RP GENE FAMILY.
RX PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA Gong J.M.;
RT "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT from the xylem sap and mediates cadmium tolerance.";
RL Plant Cell 22:1633-1646(2010).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in flowers. Detected in stems, leaves and
CC siliques. {ECO:0000269|PubMed:17481610}.
CC -!- INDUCTION: Down-regulated upon nematode infection.
CC {ECO:0000269|PubMed:16478044}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
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DR EMBL; AC002336; AAB87590.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09833.1; -; Genomic_DNA.
DR EMBL; AY063897; AAL36253.1; -; mRNA.
DR EMBL; AY094400; AAM19779.1; -; mRNA.
DR EMBL; AY150513; AAN13029.1; -; mRNA.
DR EMBL; BT002242; AAN72253.1; -; mRNA.
DR PIR; G84829; G84829.
DR RefSeq; NP_181578.1; NM_129607.4.
DR AlphaFoldDB; Q8VZR7; -.
DR SMR; Q8VZR7; -.
DR BioGRID; 3978; 5.
DR IntAct; Q8VZR7; 5.
DR STRING; 3702.AT2G40460.1; -.
DR TCDB; 2.A.17.3.23; the proton-dependent oligopeptide transporter (pot/ptr) family.
DR iPTMnet; Q8VZR7; -.
DR PaxDb; Q8VZR7; -.
DR PRIDE; Q8VZR7; -.
DR ProteomicsDB; 226436; -.
DR EnsemblPlants; AT2G40460.1; AT2G40460.1; AT2G40460.
DR GeneID; 818640; -.
DR Gramene; AT2G40460.1; AT2G40460.1; AT2G40460.
DR KEGG; ath:AT2G40460; -.
DR Araport; AT2G40460; -.
DR TAIR; locus:2061843; AT2G40460.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_1_1; -.
DR InParanoid; Q8VZR7; -.
DR OMA; EANNDYT; -.
DR OrthoDB; 365203at2759; -.
DR PhylomeDB; Q8VZR7; -.
DR PRO; PR:Q8VZR7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8VZR7; baseline and differential.
DR Genevisible; Q8VZR7; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0071916; F:dipeptide transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0042937; F:tripeptide transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0009624; P:response to nematode; HEP:TAIR.
DR CDD; cd17417; MFS_NPF5; 1.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044739; NRT1/PTR.
DR InterPro; IPR000109; POT_fam.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 2: Evidence at transcript level;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..583
FT /note="Protein NRT1/ PTR FAMILY 5.1"
FT /id="PRO_0000399964"
FT TRANSMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 405..425
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 446..466
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 485..505
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 529..549
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 98
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q05085"
FT CONFLICT 477
FT /note="E -> G (in Ref. 3; AAL36253)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 583 AA; 64824 MW; EC9CCE6BA71EA146 CRC64;
MEAAKVYTQD GTVDLQGRPV LASKTGRWRA CSFLLGYEAF ERMAFYGIAS NLVNYLTKRL
HEDTISSVRN VNNWSGAVWI TPIAGAYIAD SYIGRFWTFT ASSLIYVLGM ILLTMAVTVK
SLRPTCENGV CNKASSLQVT FFYISLYTIA IGAGGTKPNI STFGADQFDS YSIEEKKQKV
SFFNWWMFSS FLGALFATLG LVYIQENLGW GLGYGIPTVG LLVSLVVFYI GTPFYRHKVI
KTDNLAKDLV QVPIAAFKNR KLQCPDDHLE LYELDSHYYK SNGKHQVHHT PVFRFLDKAA
IKTSSRVPCT VTKVEVAKRV LGLIFIWLVT LIPSTLWAQV NTLFVKQGTT LDRKIGSNFQ
IPAASLGSFV TLSMLLSVPM YDQSFVPFMR KKTGNPRGIT LLQRLGVGFA IQIVAIAIAS
AVEVKRMRVI KEFHITSPTQ VVPMSIFWLL PQYSLLGIGD VFNAIGLLEF FYDQSPEEMQ
SLGTTFFTSG IGLGNFLNSF LVTMIDKITS KGGGKSWIGN NLNDSRLDYY YGFLVVISIV
NMGLFVWAAS KYVYKSDDDT KEFSGGGCVQ MEAKALDTSP LSI
