PTR32_ARATH
ID PTR32_ARATH Reviewed; 591 AA.
AC Q8LPL2; Q9LW69;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Protein NRT1/ PTR FAMILY 1.1;
DE Short=AtNPF1.1;
DE AltName: Full=Nitrate transporter 1.12;
GN Name=NPF1.1; Synonyms=NTR1.12; OrderedLocusNames=At3g16180;
GN ORFNames=MSL1.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-579.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [5]
RP GENE FAMILY.
RX PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA Gong J.M.;
RT "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT from the xylem sap and mediates cadmium tolerance.";
RL Plant Cell 22:1633-1646(2010).
RN [6]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, SUBCELLULAR
RP LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=24006285; DOI=10.1104/pp.113.226563;
RA Hsu P.K., Tsay Y.F.;
RT "Two phloem nitrate transporters, NRT1.11 and NRT1.12, are important for
RT redistributing xylem-borne nitrate to enhance plant growth.";
RL Plant Physiol. 163:844-856(2013).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- FUNCTION: Low-affinity nitrate transporter involved in xylem-to-phloem
CC transfer for redistributing nitrate into developing leaves. Not
CC involved in dipeptides transport. {ECO:0000269|PubMed:24006285}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=9.2 mM for nitrate {ECO:0000269|PubMed:24006285};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:24006285};
CC Multi-pass membrane protein {ECO:0000269|PubMed:24006285}.
CC -!- TISSUE SPECIFICITY: Expressed in siliques, shoots and roots. Mainly
CC detected in larger expanded leaves, in the companion cells of major
CC veins. {ECO:0000269|PubMed:17481610, ECO:0000269|PubMed:24006285}.
CC -!- DISRUPTION PHENOTYPE: Nrt1.11 and nrt1.12 double mutant is defective in
CC high-nitrate-enhanced growth. {ECO:0000269|PubMed:24006285}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB02684.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB012247; BAB02684.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE75781.1; -; Genomic_DNA.
DR EMBL; AY099590; AAM20441.1; -; mRNA.
DR RefSeq; NP_188239.1; NM_112489.3.
DR AlphaFoldDB; Q8LPL2; -.
DR SMR; Q8LPL2; -.
DR BioGRID; 6198; 11.
DR IntAct; Q8LPL2; 11.
DR STRING; 3702.AT3G16180.1; -.
DR PaxDb; Q8LPL2; -.
DR PRIDE; Q8LPL2; -.
DR ProteomicsDB; 226420; -.
DR EnsemblPlants; AT3G16180.1; AT3G16180.1; AT3G16180.
DR GeneID; 820864; -.
DR Gramene; AT3G16180.1; AT3G16180.1; AT3G16180.
DR KEGG; ath:AT3G16180; -.
DR Araport; AT3G16180; -.
DR TAIR; locus:2093442; AT3G16180.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_2_1; -.
DR InParanoid; Q8LPL2; -.
DR OMA; IDSYYWV; -.
DR OrthoDB; 365203at2759; -.
DR PhylomeDB; Q8LPL2; -.
DR SABIO-RK; Q8LPL2; -.
DR PRO; PR:Q8LPL2; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8LPL2; baseline and differential.
DR Genevisible; Q8LPL2; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0080054; F:low-affinity nitrate transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015706; P:nitrate transmembrane transport; IDA:TAIR.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR000109; POT_fam.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..591
FT /note="Protein NRT1/ PTR FAMILY 1.1"
FT /id="PRO_0000399966"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 460..480
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 496..516
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 543..563
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 591 AA; 65333 MW; 7C43618429800A68 CRC64;
MENPPDQTES KETLQQPITR RRTKGGLLTM PFIIANEGFE KVASYGLLQN MILYLMSDYR
LGLVKGQTVL FMWVAATNFM PLVGAFLSDS YLGRFLTIVI ASLSSLLGMV VLWLTAMLPQ
VKPSPCVATA GTNCSSATSS QLALLYTAFA LISIGSGGIR PCSLAFGADQ LDNKENPKNE
RVLESFFGWY YASSSVAVLI AFTVIVYIQD HLGWKIGFGI PAILMLLAGF LFVFASPLYV
KRDVSKSLFT GLAQVVAAAY VKRNLTLPDH HDSRDCYYRL KDSELKAPSD KLRFLNKACA
ISNRDEDLGS DGLALNQWRL CTTDQVEKLK ALVKVIPVWS TGIMMSINVS QNSFQLLQAK
SMDRRLSSNS TFQIPAGSFG MFTIIALISW VVLYDRAILP LASKIRGRPV RVNVKIRMGL
GLFISFLAMA VSATVEHYRR KTAISQGLAN DANSTVSISA MWLVPQYVLH GLAEALTGIG
QTEFFYTEFP KSMSSIAASL FGLGMAVANI LASVILNAVK NSSKQGNVSW IEDNINKGHY
DYYYWVLAIL SFVNVIYYVV CSWSYGPTVD QVRNDKVNGM RKEEEEVIKL N
