PTR33_ARATH
ID PTR33_ARATH Reviewed; 590 AA.
AC Q9LVE0; Q8LG02; Q9ZPE3;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 102.
DE RecName: Full=Protein NRT1/ PTR FAMILY 6.4;
DE Short=AtNPF6.4;
DE AltName: Full=Nitrate transporter 1.3;
GN Name=NPF6.4; Synonyms=NRT1.3, NTP3; OrderedLocusNames=At3g21670;
GN ORFNames=MIL23.23;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 24-590.
RA Hatzfeld Y., Saito K.;
RT "Identification of two putative nitrate transporters highly homologous to
RT CHL 1 from Arabidopsis thaliana.";
RL (er) Plant Gene Register PGR99-018(1999).
RN [6]
RP INDUCTION BY NITRATE.
RX PubMed=12668777; DOI=10.1093/pcp/pcg036;
RA Okamoto M., Vidmar J.J., Glass A.D.;
RT "Regulation of NRT1 and NRT2 gene families of Arabidopsis thaliana:
RT responses to nitrate provision.";
RL Plant Cell Physiol. 44:304-317(2003).
RN [7]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [9]
RP GENE FAMILY.
RX PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA Gong J.M.;
RT "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT from the xylem sap and mediates cadmium tolerance.";
RL Plant Cell 22:1633-1646(2010).
RN [10]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- FUNCTION: Low-affinity nitrate transporter. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves, flowers and siliques. Detected
CC in leaves. {ECO:0000269|PubMed:17481610}.
CC -!- INDUCTION: Up-regulated in the shoots by nitrate, but down-regulated in
CC the roots. {ECO:0000269|PubMed:12668777}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
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DR EMBL; AB019232; BAB02362.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76536.1; -; Genomic_DNA.
DR EMBL; AF372959; AAK50097.1; -; mRNA.
DR EMBL; AY143946; AAN28885.1; -; mRNA.
DR EMBL; AY084539; AAM61107.1; -; mRNA.
DR EMBL; AJ131464; CAB38706.1; -; mRNA.
DR PIR; T52585; T52585.
DR RefSeq; NP_188804.1; NM_113062.3.
DR AlphaFoldDB; Q9LVE0; -.
DR SMR; Q9LVE0; -.
DR STRING; 3702.AT3G21670.1; -.
DR iPTMnet; Q9LVE0; -.
DR PaxDb; Q9LVE0; -.
DR PRIDE; Q9LVE0; -.
DR ProteomicsDB; 226235; -.
DR EnsemblPlants; AT3G21670.1; AT3G21670.1; AT3G21670.
DR GeneID; 821721; -.
DR Gramene; AT3G21670.1; AT3G21670.1; AT3G21670.
DR KEGG; ath:AT3G21670; -.
DR Araport; AT3G21670; -.
DR TAIR; locus:2089935; AT3G21670.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_1_1; -.
DR InParanoid; Q9LVE0; -.
DR OMA; WNYGFVA; -.
DR OrthoDB; 365203at2759; -.
DR PhylomeDB; Q9LVE0; -.
DR PRO; PR:Q9LVE0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LVE0; baseline and differential.
DR Genevisible; Q9LVE0; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015112; F:nitrate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0010167; P:response to nitrate; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR000109; POT_fam.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 1: Evidence at protein level;
KW Membrane; Nitrate assimilation; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..590
FT /note="Protein NRT1/ PTR FAMILY 6.4"
FT /id="PRO_0000399967"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 371..391
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 419..439
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 453..473
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 492..512
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 533..553
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..23
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 17..18
FT /note="AY -> SF (in Ref. 4; AAM61107)"
FT /evidence="ECO:0000305"
FT CONFLICT 299
FT /note="D -> N (in Ref. 4; AAM61107)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 590 AA; 65246 MW; A34AEEEA3D0129F1 CRC64;
MVHVSSSHGA KDGSEEAYDY RGNPPDKSKT GGWLGAGLIL GSELSERICV MGISMNLVTY
LVGDLHISSA KSATIVTNFM GTLNLLGLLG GFLADAKLGR YKMVAISASV TALGVLLLTV
ATTISSMRPP ICDDFRRLHH QCIEANGHQL ALLYVALYTI ALGGGGIKSN VSGFGSDQFD
TSDPKEEKQM IFFFNRFYFS ISVGSLFAVI ALVYVQDNVG RGWGYGISAA TMVVAAIVLL
CGTKRYRFKK PKGSPFTTIW RVGFLAWKKR KESYPAHPSL LNGYDNTTVP HTEMLKCLDK
AAISKNESSP SSKDFEEKDP WIVSTVTQVE EVKLVMKLVP IWATNILFWT IYSQMTTFTV
EQATFMDRKL GSFTVPAGSY SAFLILTILL FTSLNERVFV PLTRRLTKKP QGITSLQRIG
VGLVFSMAAM AVAAVIENAR REAAVNNDKK ISAFWLVPQY FLVGAGEAFA YVGQLEFFIR
EAPERMKSMS TGLFLSTISM GFFVSSLLVS LVDRVTDKSW LRSNLNKARL NYFYWLLVVL
GALNFLIFIV FAMKHQYKAD VITVVVTDDD SVEKEVTKKE SSEFELKDIP
