PTR34_ARATH
ID PTR34_ARATH Reviewed; 515 AA.
AC Q9LSF0; Q0WT12;
DT 02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Protein NRT1/ PTR FAMILY 4.1;
DE Short=AtNPF4.1;
DE AltName: Full=Protein ABA-IMPORTING TRANSPORTER 3;
GN Name=NPF4.1; Synonyms=AIT3; OrderedLocusNames=At3g25260; ORFNames=MJL12.27;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP TISSUE SPECIFICITY, AND GENE FAMILY.
RX PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT "Nitrate transporters and peptide transporters.";
RL FEBS Lett. 581:2290-2300(2007).
RN [5]
RP GENE FAMILY.
RX PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA Gong J.M.;
RT "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT from the xylem sap and mediates cadmium tolerance.";
RL Plant Cell 22:1633-1646(2010).
RN [6]
RP FUNCTION.
RX PubMed=22645333; DOI=10.1073/pnas.1203567109;
RA Kanno Y., Hanada A., Chiba Y., Ichikawa T., Nakazawa M., Matsui M.,
RA Koshiba T., Kamiya Y., Seo M.;
RT "Identification of an abscisic acid transporter by functional screening
RT using the receptor complex as a sensor.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:9653-9658(2012).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA Coruzzi G., Lacombe B.;
RT "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT members in plants.";
RL Trends Plant Sci. 19:5-9(2014).
CC -!- FUNCTION: Involved in (+) and (-)-abscisic acid transport (ABA) and in
CC gibberellin import. {ECO:0000269|PubMed:22645333}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in siliques and flowers.
CC {ECO:0000269|PubMed:17481610}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC {ECO:0000305}.
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DR EMBL; AB026647; BAB02085.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77001.1; -; Genomic_DNA.
DR EMBL; AK227752; BAE99736.1; -; mRNA.
DR RefSeq; NP_189163.1; NM_113432.2.
DR AlphaFoldDB; Q9LSF0; -.
DR SMR; Q9LSF0; -.
DR STRING; 3702.AT3G25260.1; -.
DR PaxDb; Q9LSF0; -.
DR PRIDE; Q9LSF0; -.
DR ProteomicsDB; 226236; -.
DR EnsemblPlants; AT3G25260.1; AT3G25260.1; AT3G25260.
DR GeneID; 822120; -.
DR Gramene; AT3G25260.1; AT3G25260.1; AT3G25260.
DR KEGG; ath:AT3G25260; -.
DR Araport; AT3G25260; -.
DR TAIR; locus:2090325; AT3G25260.
DR eggNOG; KOG1237; Eukaryota.
DR HOGENOM; CLU_009313_4_0_1; -.
DR InParanoid; Q9LSF0; -.
DR OMA; SWCSTSM; -.
DR OrthoDB; 365203at2759; -.
DR PhylomeDB; Q9LSF0; -.
DR PRO; PR:Q9LSF0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LSF0; baseline and differential.
DR Genevisible; Q9LSF0; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR000109; POT_fam.
DR PANTHER; PTHR11654; PTHR11654; 1.
DR Pfam; PF00854; PTR2; 1.
DR SUPFAM; SSF103473; SSF103473; 2.
PE 2: Evidence at transcript level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..515
FT /note="Protein NRT1/ PTR FAMILY 4.1"
FT /id="PRO_0000399968"
FT TRANSMEM 24..44
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 298..318
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..359
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 381..401
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 413..433
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 461..481
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 492..512
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 97
FT /note="V -> F (in Ref. 3; BAE99736)"
FT /evidence="ECO:0000305"
FT CONFLICT 151
FT /note="G -> R (in Ref. 3; BAE99736)"
FT /evidence="ECO:0000305"
FT CONFLICT 312
FT /note="N -> Y (in Ref. 3; BAE99736)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 515 AA; 57161 MW; B8B4CF08A7528771 CRC64;
MQIEMEEKFE DWRGKEAISG KHGGIKAAFI ACVVETMENM VFLACSTNFM MYFTKSMNYS
TPKAATMVTN FVGTSFLLTI FGGFVADSFL TRFAAFVLFG SIELLGLIML TLQAHITKLQ
PQGGKKPSTP QSTVLFTGLY AIAIGVGGVK GSLPAHGGDQ IGTRNQRLIS GFFNWYFFSV
CLGGFLAVTL MVWIEENIGW SSSFTISTAV LASAIFVFVA GCPMYRFKRP AGSPLTRIVN
VFVSAARNRN RFVTDAEVVT QNHNSTDKSI HHNKFKFLNK AKLNNKISAT QVEETRTFLA
LLPIFGSTII MNCCVAQMGT FSVQQGMVTN RKLSRSFEIP VASLNAIPLL CMLSSLALYE
LFGKRILSNS ERSSSFNLKR IGYGLALTSI SMAVAAIVEV KRKHEAVHNN IKISVFWLEL
QFVMLSLSDM LTVGGMLEFF FRESPASMRS MSTALGWCST AMGFFLSSVL VEVVNGITGW
LRDDLNESRL ELFYLVLCVL NTLNLFNYIF WAKRY
