ID   PTR35_ARATH             Reviewed;         521 AA.
AC   Q9LSE8;
DT   02-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Protein NRT1/ PTR FAMILY 4.2;
DE            Short=AtNPF4.2;
DE   AltName: Full=Protein ABA-IMPORTING TRANSPORTER 4;
GN   Name=NPF4.2; Synonyms=AIT4; OrderedLocusNames=At3g25280; ORFNames=MJL12.24;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   TISSUE SPECIFICITY, AND GENE FAMILY.
RX   PubMed=17481610; DOI=10.1016/j.febslet.2007.04.047;
RA   Tsay Y.F., Chiu C.C., Tsai C.B., Ho C.H., Hsu P.K.;
RT   "Nitrate transporters and peptide transporters.";
RL   FEBS Lett. 581:2290-2300(2007).
RN   [4]
RP   GENE FAMILY.
RX   PubMed=20501909; DOI=10.1105/tpc.110.075242;
RA   Li J.Y., Fu Y.L., Pike S.M., Bao J., Tian W., Zhang Y., Chen C.Z.,
RA   Zhang Y., Li H.M., Huang J., Li L.G., Schroeder J.I., Gassmann W.,
RA   Gong J.M.;
RT   "The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal
RT   from the xylem sap and mediates cadmium tolerance.";
RL   Plant Cell 22:1633-1646(2010).
RN   [5]
RP   FUNCTION.
RX   PubMed=22645333; DOI=10.1073/pnas.1203567109;
RA   Kanno Y., Hanada A., Chiba Y., Ichikawa T., Nakazawa M., Matsui M.,
RA   Koshiba T., Kamiya Y., Seo M.;
RT   "Identification of an abscisic acid transporter by functional screening
RT   using the receptor complex as a sensor.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:9653-9658(2012).
RN   [6]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=24055139; DOI=10.1016/j.tplants.2013.08.008;
RA   Leran S., Varala K., Boyer J.C., Chiurazzi M., Crawford N.,
RA   Daniel-Vedele F., David L., Dickstein R., Fernandez E., Forde B.,
RA   Gassmann W., Geiger D., Gojon A., Gong J.M., Halkier B.A., Harris J.M.,
RA   Hedrich R., Limami A.M., Rentsch D., Seo M., Tsay Y.F., Zhang M.,
RA   Coruzzi G., Lacombe B.;
RT   "A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family
RT   members in plants.";
RL   Trends Plant Sci. 19:5-9(2014).
CC   -!- FUNCTION: Involved in abscisic acid transport.
CC       {ECO:0000269|PubMed:22645333}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC       protein {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in siliques.
CC       {ECO:0000269|PubMed:17481610}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. Proton-
CC       dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB026647; BAB02087.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE77004.1; -; Genomic_DNA.
DR   RefSeq; NP_189165.1; NM_113434.1.
DR   AlphaFoldDB; Q9LSE8; -.
DR   SMR; Q9LSE8; -.
DR   TCDB; 2.A.17.3.12; the proton-dependent oligopeptide transporter (pot/ptr) family.
DR   PaxDb; Q9LSE8; -.
DR   PRIDE; Q9LSE8; -.
DR   ProteomicsDB; 226421; -.
DR   EnsemblPlants; AT3G25280.1; AT3G25280.1; AT3G25280.
DR   GeneID; 822122; -.
DR   Gramene; AT3G25280.1; AT3G25280.1; AT3G25280.
DR   KEGG; ath:AT3G25280; -.
DR   Araport; AT3G25280; -.
DR   TAIR; locus:2090230; AT3G25280.
DR   eggNOG; KOG1237; Eukaryota.
DR   HOGENOM; CLU_009313_4_0_1; -.
DR   InParanoid; Q9LSE8; -.
DR   OMA; LNYNFWM; -.
DR   OrthoDB; 365203at2759; -.
DR   PhylomeDB; Q9LSE8; -.
DR   PRO; PR:Q9LSE8; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LSE8; baseline and differential.
DR   Genevisible; Q9LSE8; AT.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR000109; POT_fam.
DR   PANTHER; PTHR11654; PTHR11654; 1.
DR   Pfam; PF00854; PTR2; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..521
FT                   /note="Protein NRT1/ PTR FAMILY 4.2"
FT                   /id="PRO_0000399969"
FT   TRANSMEM        30..50
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        65..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        133..153
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..192
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        204..224
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        297..317
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        338..358
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        381..401
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        413..433
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        451..471
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        498..518
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   521 AA;  58353 MW;  750C7125E993BEA1 CRC64;
     MENDMEEKFE DWKGKEAIPG KHGGIRAASI VCVVVMMENI VFIANGFNFV KYFMGSMHYT
     PATAANMVTN FMGTSFLLTL FGGFIADSFV THFTTFIVFC CIELMGLILL TFQAHNPKLL
     PEKDKTPSTL QSAILFTGLY AMAIGTGGLK ASLPSHGGDQ IDRRNPRLIS RFFDWLYFSI
     CSGCLLAVTV VLWIEEKKGW IWSFNISVGI LATALCIFTV GLPFYRFKRP NGSPLKKIAI
     VIISAARNRN KSDLDEEMMR GLISIYKNNS HNKLKWIDKA TLNKNISETE VEETRTFLGL
     LPIFGSTIVM SCCVAQLSTF SAQQGMLMNK KLFHSFEIPV PSLTAIPLIF MLLSIPLYEF
     FGKKISSGNN NRSSSFNLKR IGLGLALSSV SMAVSAIVEA KRKHEVVHNN FRISVLWLVF
     QYLMLSVSDM LTLGGMLEFF YREAPSNMKS ISTALGWCST ALGFFLSTTL VEVTNAVTGR
     LGHQWLGGED LNKTRLELFY VLLCVLNTLN LLNYIFWAKR Y